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http://dx.doi.org/10.4490/ALGAE.2006.21.1.125

Long-term Preservation of Bloom-forming Cyanobacteria by Cryopreservation  

Park, Hae-Kyung (Han River Environment Research Center, National Institute of Environmental Research)
Publication Information
ALGAE / v.21, no.1, 2006 , pp. 125-131 More about this Journal
Abstract
Long-term preservation of bloom-forming cyanobacteria was evaluated using cryopreservation and freeze-drying of nine strains belonging to four genera and seven species. All test strains, except Aphanizomenon flos-aquae NIER- 10028, showed partial or complete survival following cryopreservation and freeze-drying. Frozen and freeze-dried strains were preserved for more than two years and were revived monthly. Most strains showed higher post-thaw viability after cryopreservation, especially without cryoprotectant compared to freeze-drying. Microcystis aeruginosa NIER-10010, M. viridis NIER-10020, M. ichthyoblabe NIER-10023, M. novacekii NIER-10029 and Oscillatoria sancta NIER-10027 were revived after 2.5 years of cryopreservation. These results suggest that cryopreservation may be an easy and timesaving long-term preservation method for bloom-forming cyanobacteria.
Keywords
cryopreservation; cyanobacteria; freeze-drying; Microcystis; Oscillatoria;
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