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http://dx.doi.org/10.7740/kjcs.2021.66.4.326

Discrimination and Hordein Polypeptide Patterns of Malting Barley Varieties Using UPLC  

Yoon, Young-Mi (National Institute of Crop Science, RDA)
Kim, Yang-Kil (National Institute of Crop Science, RDA)
Kang, Chon-Sik (National Institute of Crop Science, RDA)
Park, Jin-Cheon (National Institute of Crop Science, RDA)
Park, Tae-Il (National Institute of Crop Science, RDA)
Publication Information
KOREAN JOURNAL OF CROP SCIENCE / v.66, no.4, 2021 , pp. 326-338 More about this Journal
Abstract
Ultra-performance liquid chromatography (UPLC) was used to assess the hordein protein fraction of malting barley. C-hordeins (barley prolamins) were extracted with 70% ethanol (EtOH) and 55% isopropyl alcohol (IPA, 2-propanol), and B-hordeins were extracted with the same alcohols in 1.0% dithiothreitol (DTT). High molecular weight (HMW) prolamins (D-hordeins) were extracted with 50% IPA with 1M Tris-HCl (pH 8.0). The same protein patterns were observed in both the experimental extraction solutions (EtOH and IPA). However, the patterns of hordein, extracted with EtOH and IPA containing 1.0% DTT, differed slightly. C- and B-hordeins extracted from those solutions were analyzed. Twenty-six malting barley varieties developed in Korea were analyzed using UPLC. The varieties were divided into seven groups according to hordein patterns of retention time 16 min to 18 min, and 20 varieties showed unique patterns.
Keywords
hordein; malting barley; UPLC; variety;
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