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http://dx.doi.org/10.7740/kjcs.2020.65.2.104

Analysis of Growth Response and Gene Expression by Waterlogging Stress on B73 Maize  

Go, Young Sam (Department of Central Area Crop Science, National Institute of Crop Science, Rural Development Administration)
Kim, Jung-Tae (Planning and Coordination Division, National Institute of Crop Science, Rural Development Administration)
Bae, Hwan Hee (Department of Central Area Crop Science, National Institute of Crop Science, Rural Development Administration)
Son, Beom-Young (Department of Central Area Crop Science, National Institute of Crop Science, Rural Development Administration)
Yi, Gibum (Department of Central Area Crop Science, National Institute of Crop Science, Rural Development Administration)
Ha, Jun Young (Department of Central Area Crop Science, National Institute of Crop Science, Rural Development Administration)
Kim, Sun-Lim (Department of Central Area Crop Science, National Institute of Crop Science, Rural Development Administration)
Baek, Seong-Bum (Department of Central Area Crop Science, National Institute of Crop Science, Rural Development Administration)
Publication Information
KOREAN JOURNAL OF CROP SCIENCE / v.65, no.2, 2020 , pp. 104-112 More about this Journal
Abstract
Maize is thought to be an alternative crop to rice in paddy fields for efficient field management and maintenance of rice production at appropriate levels in Korea. Thus efforts to breed waterlogging-tolerant maize cultivars have been ongoing. However, molecular studies related to waterlogging tolerance are limited for developing molecular markers to select waterlogging tolerant maize varieties. In this study, we examined molecular biological changes of B73 in the V3 stage after immersion treatment for 7 days. Overall growth of maize was lower in treated samples compared to non-immersed control samples. The length of leaf and root decreased by 21.3% and 50.6%, respectively and the weight of root reduced by 21.6%. Soil plant analysis development (SPAD) value and chlorophyll content of leaf also decreased by 55.7% and 35.3%, respectively. Reactive oxygen species (ROS) content of root increased by 46.5% at 2 hours in immersion treatment. In addition, immersed roots were 2.5-fold thickened with additional aerenchyma formation in the cortex. In order to identify the causes of these changes, we performed a microarray and found increased expression of genes, such as WIP1, PMIP2, EXPA1, TPS1, and MAS1, in immersed samples. These differentially expressed genes and expression of previously reported genes, including ALDH2, Wusl1032, UP-1, UP-2, and CAT2 were further confirmed with qRT-PCR. Here, we report 7 differentially expressed genes after immersing treatment, which may be utilized as useful resources for breeding waterlogging- tolerant maize.
Keywords
B73 maize; gene expression; growth response; waterlogging;
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1 Alamgir, H. and S. N. Uddin. 2011. Mechanisms of waterlogging tolerance in wheat: morphological and metabolic adaptations under hypoxia or anoxia. Aust. J. Crop Sci. 5(9) : 940-1110.
2 Arora, K., K. K. Panda, S. Mittal, M. G. Mallikarjuna, R. R. Atmakuri, K. D. Prasanta, and T. Nepolean. 2017. RNAseq revealed the important gene pathways controlling adaptive mechanisms under waterlogged stress in maize. Sci. Rep. 7 : 10950.   DOI
3 Ashraf, M. A., M. S. Ahmad, M. Ashraf, F. Al-Qurainy, and M. Y. Ashraf. 2011. Alleviation of waterlogging stress in upland cotton (Gossypium hirsutum L.) by exogenous application of potassium in soil and as a foliar spray. Crop Pasture Sci. 62 : 25-38.   DOI
4 Boru, G., T. Vantoai, J. Alves, D. Hua, and M. Knee. 2003. Response of soybean to oxygen deficiency and elevated root-zone carbon dioxide concentration. Ann. Bot. 91(4) : 447-453.   DOI
5 Chalivendra, C. S. and M. S. Martin. 2003. Molecular and cellular adaptations of maize to flooding stress. Ann. Bot. 91(2) : 119-127.   DOI
6 Christianson, J. A., D. J. Llewellyn, E. S. Dennis, and I. W. Wilson. 2010. Global gene expression responses to waterlogging in roots and leaves of cotton (Gossypium hirsutum L.). Plant Cell Physiol. 51(1) : 21-37.   DOI
7 Gill, M. B., F. Zeng, L. Shabala, G. Zhang, M. Yu, V. Demidchik, S. Shabala, and M. Zhou. 2019. Identification of QTL related to ROS formation under hypoxia and their association with waterlogging and salt tolerance in barley. Int. J. Mol. Sci. 20(3) : 699-714.   DOI
8 Komatsu, S., C. Han, Y. Nanjo, M. Altaf-Un-Nahar, K. Wang, D. He, and P. Yang. 2013. Label-free quantitative proteomic analysis of abscisic acid effect in early-stage soybean under flooding. J. Proteome Res. 12(11) : 4769-4784.   DOI
9 Kong, F., A. Oyanagi, and S. Komatsu. 2010. Cell wall proteome of wheat roots under flooding stress using gel-based and LC MS/MS based proteomics approaches. Biochim. Biophys. Acta 1804(1) : 124-136.   DOI
10 Koo, S. C, H. T. Kim, B. K. Kang, Y. H. Lww, K. W. Oh, H. Y. Kim, I. Y. Back, H. T. Yun, and M. S. Choi. 2014. Screening of Flooding Tolerance in Soybean Germplasm Collection. Korean J. Breed. Sci. 46(2) : 129-135   DOI
11 Liu, F., T. Vantoai, G. Bock, L.D. Linford, and J. Quackenbush. 2005. Global transcription profiling reveals novel insights into hypoxic response in Arabidopsis. Plant Physiol. 137 : 1115-1129.   DOI
12 Liu, Z., K. Sunita, L. Zhang, and W. Doreen. 2012. Characterization of miRNAs in response to short-term waterlogging in three inbred lines of Zea mays. PLoS ONE 7(6) : e39786.   DOI
13 Ren, B. Z., J. W. Zhang, X. Li, X. Fan, S. T. Dong, B. Zhao, and P. Liu. 2014. Effect of waterlogging on leaf senescence characteristics of summer maize in the field. J. Appl. Ecol. 25(4) : 1022-1028.
14 Moon, J., S. Shin, H. C. Kim, K. Song, J. Y. Kim, K. Kim, and B. Lee. 2018. Assessment of the candidate genes of expression markers associated with drought stress in maize seedlings. Korean J. Breed. Sci. 50(3) : 224-235.   DOI
15 Qiu, F. Z., Y. L. Zheng, Z. L. Zhang, and S. Z. Xu. 2007. Mapping of QTL associated with waterlogging tolerance during the seedling stage in maize. Ann. Bot. 99(6) : 1067-1081.   DOI
16 Ren, B., J. Zhang, S. Dong, P. Liu, and B. Zhao. 2016. Effects of waterlogging on leaf mesophyll cell ultrastructure and photosynthetic characteristics of summer maize. PLoS ONE 11(9) : e0161424.   DOI
17 Salah, A., J. Li, J. Ge, C. Cao, H. Li, Y. Wang, Z. Liu, M. Zhan, and M. Zhao. 2019. Morphological and physiological responses of maize seedlings under drought and waterlogging. J. Agr. Sci. Tech. 21(5) : 1199-1214.
18 Sallam, A. and H. D. Scott. 1987. Effects of prolonged flooding on soybeans during early vegetative growth. Soil Sci. 144(1) : 61-66.   DOI
19 Tang, B., S. Xu, X. Zou, Y. Zheng, and F. Qiu. 2010. Changes of antioxidative enzymes and lipid peroxidation in leaves and roots of waterlogging-tolerant and waterlogging-sensitive maize genotypes at seedling stage. Agric. Sci. China. 9 : 651-661.   DOI
20 Yamauchi, T., I. Rajhi, and M. Nakazono. 2011. Lysigenous aerenchyma formation in maize root is confined to cortical cells by regulation of genes related to generation and scavenging of reactive oxygen species. Plant Signal. Behav. 6(5) : 759-761.   DOI
21 Yordanova, R. Y, K. G Zheng, Z. G. Stoinova, and L. P. Popova. 2004. Changes in the leaf polypeptide patterns of barley plants exposed to soil flooding. Biologia Plantarum 48(2) : 301-304.   DOI