Browse > Article

Comparison of Agronomic Characteristics and Activity Variation of ADP-Glucose Pyrophosphorylase at Different Growth Stages in Soybean Cultivars  

Kim, Young-Jin (Div. of Winter Cereal and Forage Crop Research, National Institute of Crop Science, RDA)
Lee, Si-Myeong (Div. of Agricultural Bio-resources, National Academy of Agricultural Science, RDA)
Cho, Sang-Kyun (Div. of Winter Cereal and Forage Crop Research, National Institute of Crop Science, RDA)
Oh, Young-Jin (Div. of Winter Cereal and Forage Crop Research, National Institute of Crop Science, RDA)
Kim, Hag-Sin (Div. of Winter Cereal and Forage Crop Research, National Institute of Crop Science, RDA)
Publication Information
KOREAN JOURNAL OF CROP SCIENCE / v.55, no.2, 2010 , pp. 139-143 More about this Journal
Abstract
The relationship between ADP-glucose pyrophosphorylase (AGP) activity and the characteristics of related pod setting in developing seed of soybean cv. Pungsannamulkong, Iksannamulkong, Geumjeongkong #1 and Danpaheuk was studied. AGP activity during the accumulate of the majority of dry matter in all cultivars suggested that this enzyme might be associated with this process. At the Vn and R1 stages, AGP activity of full-grown leaves of Pungsannamulkong, Iksannamulkong, Geumjeongkong #1 was the highest and then decreased progressively. However AGP activity of Danpaheuk was the lowest and also had lower seed weight. So regulation of matter accumulation in developing soybean seeds may also depend on AGP activity. AGP capacities as expressed by AGP activity seem to have a good predicting value for the dry matter of leaf and seed at R1 to R5 stages in our series of R3 stage genotypes. Western blots probed with antibody specific to the subunit of potato AGP revealed a single 60KD immunoreactive band that changed in intensity during the growth cycle in association with changes in total AGP activity.
Keywords
Soybean; AGP activity; pod setting; developing seed; seed weight; Western blot;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Mian, M. A. R., R. Wells, T. E. Carter Jr., D. A. Ashley, and H. R. Boerma. 1998. RFLP tagging of QTLs conditioning specific leaf weight and leaf size in soybean. Theo. and Appl. Gen. 96:354-360.   DOI   ScienceOn
2 Muller-Rober B., U. L. Cognata, U. Sonnewald and L. Willmitzer. 1994. A truncated version of an ADP-glucose pyrophosphorylase promoter from potato specifies guard cell-selective expression in transgenic plants. The Plant Cell 6(5):601-612.   DOI
3 Park, SW. and Chung, WI. 1998. Molecular cloning and organspecific expression of three isoforms of tomato ADP-glucosepyrophosphorylase gene. Gene 206:215–221.
4 Preiss J. 1991. Biology and molecular biology of starch synthesis and its regulation. Oxford Surv Plant Mol Cell Biol. 7:59-114.
5 山口淳一, 河內和, 田中明. 1975. 作物 生長效率に 關する 硏究 (第5報). 日本土肥雜誌 46(4):120-125.
6 Zhangying W., C. Xiaoping , W. Jianhua, L. Tingsong, L. Yan, Z. Li and W. Guoying. 2007. Increasing maize seed weight by enhancing the cytoplasmic ADP-glucose pyrophosphorylase activity in transgenic maize plants. Plant Cell, Tissue and Organ Culture 88(1):83-92.   DOI   ScienceOn
7 김동헌, 이시명, 황영수. 1998. 식물의 전분합성 관련효소 특성 구명 연구. 농업과학원 시험연구사업보고서(생물자원부편) pp. 488-495.
8 Shibles, R. J. Secor, and D. M. Ford. 1987. Carbon assimilation and metabolism. In JR Wilcox, ed, Soybeans: Improvement production, and uses. Amer. Soc. of Agron., Madison, WI, pp. 535-588.
9 Huber SC. 1983. Role of sucrose-phosphate synthase in partitioning of carbon in leaves. Plant Physiol. 71:818-821.   DOI   ScienceOn
10 Sowokinos JR. 1976. Pyrophospholylase in Solanum tuberosum I. Changes in ADP-glucose and UDP-glucose pyrophosphorylase activities associated with starch biosynthesis during tuberization, maturation and storage of potatoes. Plant Physiol. 57:63-68.   DOI   ScienceOn
11 Crafts-Brandner SJ. 1992. Phosphorus nutrition influence on starch and sucrose accumulation, and activities of ADPglucose pyrophosphorylase and sucrose-phosphate synthase during the grain filling period in soybean. Plant Physiol. 98:1133-1138.   DOI   ScienceOn
12 Keeling PL, Wood JR, Tyson RH and Bridges IG. 1988. Starch biosynthesis in developing wheat grain. Plant Physiol. 87: 311-319.   DOI   ScienceOn
13 Kerr PS, Huber SC, and Israel DW. 1984. Effect of source on soybean leaf sucrose phosphate synthase starch formation and whole plant growth. Plant Physiol. 75:483-488.   DOI   ScienceOn
14 Choi SB, Kim KH, Kavakli IH, Lee SK and Okita TW. 2001. Transcriptional expression characteristics and subcellular localization of ADP-glucose pyrophosphorylase in the oil plant Perilla frutescens. Plant and Cell Physiol. 42(2):146-153   DOI
15 김영진, 이강세, 천상욱, 오영진, 김경호, 최재성, 이문희. 2003. 엽형에 따른 콩 품종의 광합성 능력과 잎의 해부형태 비교. 한국작물학회지 48(3):248-251.   과학기술학회마을