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In vitro Biological Activities of Anthocyanin Crude Extracts from Black Soybean  

Lee, Hye-Jeong (Dept. of Food and Nutrition, Gachon University of Medicine and Science)
Do, Wan-Nyeo (Dept. of Food and Nutrition, Kangneung University)
Kim, Yong-Ho (Dept. of Medical Biotechnology, Soonchunhyang University)
Publication Information
KOREAN JOURNAL OF CROP SCIENCE / v.55, no.1, 2010 , pp. 65-69 More about this Journal
Abstract
This study was carried out to investigate the antioxidative and anti-inflammatory activity of crude anthocyanin compounds extracted from black soybean. The crude anthocyanin compounds were extracted with 80% methanol and concentrated to powder. The most abundant compound isolated from the extract was C3G(cyanidin-3-glucoside). The superoxide dismutase (SOD) assay was conducted to assess the antioxidative activity of the crude extract. SOD, which catalyzes the dismutation of the superoxide anion into hydrogen peroxide and molecular oxygen, is one of the most important antioxidative enzymes. The black soybean anthocyanin extracts inhibited more than 90% of the superoxide radical at a concentration of 0.1% and 100% at a concentration of 0.5%, indicating that this extract displayed excellent antioxidative activity. To assess the anti-inflammatory activity of the extract, a NO(Nitric oxide) production assay in RAW 264.7 cells was performed. NO is an important physiological messenger and effector molecule in many biological systems, including immunological, neuronal and cardiovascular tissues. In this assay, the anthocyanin extracts showed a high anti-inflammatory potential, where the inhibitory potency for NO production was similar to the positive control, particularly for EGCG(epigallocatechin-3-gallate), which is known to have excellent anti-inflammatory activity. Thus, it can be concluded that the anthocyanin extracts from black soybean have distinctive pharmaceutical activities and may be used as an excellent source materials to supplement the health benefits of various food products.
Keywords
black soybean; anthocyanin; C3G; SOD activity; NO production;
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