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Influence of Antibiotics on Shoot Regeneration and Agrobacteium Suppression Using Cotyledonary Node in Korean Soybean Cultivars  

Kim, Kyung-Hee (Department of Plant Biotechnology, Dongguk University)
Lee, Jae-Eun (National Institute of Crop Science, RDA)
Kwon, Young-Up (National Institute of Crop Science, RDA)
Lee, Byung-Moo (Department of Plant Biotechnology, Dongguk University)
Publication Information
KOREAN JOURNAL OF CROP SCIENCE / v.54, no.3, 2009 , pp. 307-313 More about this Journal
Abstract
Mature dry seeds of Korean cultivars, Daepungkong, Muhankong, Myeongjunamulkong, Somyeongkong, Sowonkong, Jinpumkong, and Pungsannamulkong were used. The influence of antibiotics on elimination of Agrobacterium growth and shoot regeneration was estimated with cotyledonary node. Cefotaxime and timentin at the concentration of 250 and 500 mg/l suppressed Agrobacterium, especially cefotaxime was an efficient antibiotic to suppress Agrobacterium in all cultivars. While carbenicillin and timentin at the concentration of 50 and 100 mg/l were not sufficient to control the development of Agrobacterium, respectively. Cefotaxime and timentin represented high shoot formation rates compared with carbenicillin. Carbenicillin at low concentrations did not effectively suppress Agrobacterium and also had no effect on shoot development. Cefotaxime at the concentration of 250 mg/l showed maximum frequency of shoot regeneration in cvs. Somyeongkong and Sowonkong. Furthermore, on medium containing cefotaxime, shoot was more quickly formed than the other antibiotics. The use of cefotaxime was very useful for elimination of Agrobacterium growth with cotyledonary node of Korean soybean cultivars.
Keywords
antibiotics; soybean; cotyledonary node; regeneration; Agrobacterium suppression;
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1 Borrelli, G. M., N. Di Fonzo., and E. Lupotto. 1992. Effect of cefotaxime on callus culture and plant regeneration in durum wheat. J. Plant Physiol. 140 : 372-374   DOI
2 Cheng, T. Y., H. Saka., and T. H. Voqui-Dinh. 1980. Plant regeneration from soybean cotyledonary node segments in culture. Plant Sci. Lett. 19 : 91-99   DOI   ScienceOn
3 Gamborg, O. L., R. A. Miller, and K. Ojiama. 1968. Nutrient requirements of suspension cultures of soybean root cells. Exp. Cell Res. 50 : 151-158   DOI   ScienceOn
4 Han, S. N., P. R. Oh, H. S. Kim, H. Y. Heo, J. C. Moon, S. K. Lee, K. H. Kim, Y. W. Seo, and B. M. Lee. 2007. Effect of antibiotics on suppression of Agrobacterium tumefaciens and plant regeneration from wheat embryo. J. Crop Sci. Biotech 10(2): 92-97
5 Nauerby, B., K. Billing, and R. Wyndaele. 1997. Influence of the antibiotic timentin on plant regeneration compared to carbenicillin and cefotaxime in concentration suitable for elimination of Agrobacterium tumefaciens. Plant Sci. 123 : 169-177   DOI   ScienceOn
6 Santarem, E. R., H. N. Trick, J. S. Essig, and J. J. Finer. 1998. Sonication-assisted Agrobacterium-mediated transformation of soybean immature cotyledons: optimization of transient expression. Plant Cell Rep. 17 : 752-759   DOI   ScienceOn
7 McCabe, D. E., W. F. Swain, B. J. Martinell, and P. Christou. 1988. Stable transformation of soybean (Glycine max) by particle acceleration. Biotechnology 6 : 923-926   DOI
8 Kartha, K. K., K. Pahl, N. L. Leung, and L. A. Mroginski. 1981. Plant regeneration from meristems of grain legumes: soybean, cowpea, peanut, chickpea, and bean. Can J. Bot. 59 : 1671-1679   DOI
9 Nakano, M. and M. Mii. 1993. Antibiotics stimulate somatic embryogenesis without plant growth regulators in several Dianthus cultivars. Plant Physiology 141 : 721-725   DOI   ScienceOn
10 Mathias, R. J. and C. Mukasa. 1987. The effect of cefotaxime on the growth and regeneration of callus from four varieties of barley (Hordeum vulgare L.). Plant Cell Rep. 6 : 454-457   DOI   ScienceOn
11 Ling, H. Q., D. Kriseleit, and M. W. Ganal. 1998. Effect of ticarcillin/potassium calvulanate on callus growth and shoot regeneration in Agrobacterium-mediated transformation of tomato (Lycopersicon esculentum Mill.). Plant Cell Rep. 17 : 843-847   DOI   ScienceOn
12 Droste, A., G. Pasquali, and M. H. Bodanese-Zanettini. 2002. Transgenic fertile plants of soybean [Glycine max (L). Merrill] obtained from bombarded embryogenic tissue. Euphytica 127 : 367-376   DOI   ScienceOn
13 Hinchee, M. A. W., D. V. Connor-Ward, C. A. Newell, R. E. McDonnell, S. J. Sato, C. S. Gasser, D. A. Fischhoff, D. B. Re, R. T. Fraley, and R. B. Horsch. 1998. Production of transgenic soybean plants using Agrobacterium-mediated DNA transfer. Biotechnology 6 : 915-922   DOI
14 Tang, W., H. Luo, and R. J. Newton. 2004. Effects of antibiotics on the elimination of Agrobacterium tumefaciens from loblolly pine (Pinus taeda) zygotic embryo explants and on transgenic plant regeneration. Plant Cell, Tiss. Organ Cult. 70 : 71-81   DOI
15 Cheng, Z. M., J. A. Schnurr., and J. A. Kapaun. 1998. Timentin as an alternative antibiotic for suppression of Agrobacterium tumefaciens in genetic transformation. Plant Cell Rep. 17 : 646-649   DOI   ScienceOn
16 Wiebke, B., F. Ferreira, G. Pasquali, M. H. Bodanese-Zanettini, and A. Droste. 2006. Influence of antibiotics on embryogenic tissue and Agrobacterium tumefaciens suppression in soybean genetic transformation. Bragantia 65(4) : 543-551   DOI
17 Yepes, L. M. and H. S. Aldwinckle. 1994. Factors that affect leaf regeneration efficiency in apple, and effect of antibiotics in morphogenesis. Plant Cell Tissue Organ Cult. 37 : 257-269
18 Trick, H. N. and J. J. Finer. 1998. Sonication-assisted Agrobacterium- mediated transformation of soybean [Glycine max (L.) Merrill] embryogenic suspension cultures. Plant Cell Rep. 17 : 482-488   DOI   ScienceOn
19 Ma, X. H. and T. L. Wu. 2008. Rapid and efficient regeneration in soybean [Glycine max (L.) Merrill] from whole cotyledonary node explants. Acta. Physiol. plant 30 : 209-216   DOI   ScienceOn
20 Mayolo, G. A., S. N. Maximova, S. Piskak, and M. J. Guiltinan. 2003. Moxalactam as a counter-selection antibiotic for Agrobacterium-mediated transformation and its positive effects on Theobroma cacao somatic embryogenesis. Plant Science 164 : 607-615   DOI   ScienceOn
21 Sato, S., C. Newell, K. Kolacz, L. Tredo, J. Finer, and M. Hinchee. 1993. Stable transformation via particle bombardment in two different soybean regeneration system. Plant Cell Rep. 12 : 408-413   DOI   ScienceOn
22 Sarma, K. S., N. E. Evans, and C. Selby. 1995. Effect of carbenicillin and cefotaxime on somatic embryogenesis of Skita spruce [Picea sitchensis (Bong.) Carr.]. J. Exp. Bot. 45 :1779-1781
23 Wright, M. S., D. V. Ward, M. A. Hinchee, M. G. Carnes, and R. J. Kaufman. 1987. Regeneration of soybean (Glycine max L. Merr.) from cultured primary leaf tissue. Plant Cell Rep. 6 : 83-90
24 Danilova, S. A., and Yu. I. Dolgikh. 2004. The stimulatory effect of the antibiotic cefotaxime on plant regeneration in maize tissue culture. Russ. J. Plant Physiol. 51(4) : 559-562
25 Pollock, K., D. G. Barfield, and R. Shield. 1983. The toxicity of antibiotics to plant cell cultures. Plant Cell Rep. 2 : 36-39   DOI   ScienceOn
26 Margie, M. P., H. Shou, Z. Guo, Z. Zhang, A. K. Banerjee, and K. Wang. 2004. Assessment of conditions affecting Agrobacterium-mediated soybean transformation using the cotyledonary node explants. Euphytica 136 : 167-179   DOI   ScienceOn
27 Sato, S., A. Xing, X. Ye, B. Schweiger, A. Kinney, G. Graef, and T. Clement. 2004. Production of linoleic acid and stearidonic acid in seeds of marker-free transgenic soybean. Crop Sci. 44 : 646-652   DOI   ScienceOn
28 Margie, M. P., J. C. Martinez, A. B. Kalvig, T. M. Fonger, and K. Wang. 2006. Improved cotyledonary node method using an alternative explants derived from mature seed for efficient Agrobacterium-mediated soybean transformation. Plant Cell Rep. 25 : 206-213   DOI   ScienceOn
29 Olhoft, P. M., L. E. Flagel, C. M. Donovan, and D. A. Somers. 2003. Efficient soybean transformation using hygromycin B selection in the cotyledonary-node method. Planta 216 : 723-735   DOI   PUBMED
30 Olhoft, P. M. and D. A. Somers. 2001. L-Cysteine increases Agrobacterium-mediated T-DNA delivery into soybean cotyledonary-node cells. Plant Cell Rep. 20 : 706-711   DOI   ScienceOn
31 Wright, M. S., S. M. Koehler, M. A. Hinchee, and M. G. Carnes. 1986. Plant regeneration by organogenesis in Glycine max. Plant Cell Rep. 5 : 150-154   DOI   ScienceOn
32 Liu, H. K., C. Yang, and Z. M. Wei. 2004. Efficient Agrobacterium tumefaciens-mediated transformation of soybeans using an embryonic tip regeneration system. Planta 219 : 1042-1049   DOI   ScienceOn
33 Agrawal, D. C., A. K. Banerjee, P. H. Kedari, S. Jacob, S. Hazra, and K. V. Krishnamurthy. 1998. Effect of cefotaxime on the growth of excised embryo-axes of 6 cultivars of cotton (Gossypium hirsutum L.). J. Plant Physiol. 152 : 580-582   DOI   ScienceOn
34 Franklin, G., L. Carpenter, E. Davis, C. S. Reddy, D. Al-Abed, W. Abou Alaiwi, M. Parani, B. Smith, S. L. Goldman, and R. V. Sairam. 2004. Factors influencing regeneration of soybean from mature and immature cotyledons. Plant Growth Regul. 43 : 73-79   DOI   ScienceOn
35 Shackelford, N. J. and C. A. Chlan. 1996. Identification of antibiotics that are effective in eliminating Agrobacterium tumefaciens. Plant Molecular Biology Reporter 14 : 50-57   DOI
36 Barwale, U. B., H. R. Kerns, and J. M. Widholm. 1986. Plant regeneration from callus cultures of several soybean via embryogenesis and organogenesis. Planta 167 : 473-481   DOI   ScienceOn
37 Mathias, R. J. and Boyd L. A. 1986. Cefotaxime stimulates callus growth, embryogenesis ad regeneration in hexaploid bread wheat (Triticum aestivum L. Em. Thell). Plant Sci. 46 : 217-223   DOI   ScienceOn
38 Murashige, T. and F. Skoog. 1962. A revised medium for rapid growth and bioassay with tobacco tissue culture. Physiol. Plant 15 : 473-497   DOI