Browse > Article

QTL Analysis of Protein Content in Double-haploid Lines of Rice  

Qin, Yang (Department of Agronomy, Kyungpook National University)
Kim, Suk-Man (Department of Agronomy, Kyungpook National University)
Sohn, Jae-Keun (Department of Agronomy, Kyungpook National University)
Publication Information
KOREAN JOURNAL OF CROP SCIENCE / v.54, no.2, 2009 , pp. 165-171 More about this Journal
Abstract
The objective of this study was to characterize the main-effect QTLs, epistatic QTLs and QTL-by-environment interactions (QE), which are involved in the control of protein content. A population of 120 doubled haploid (DH) lines derived from a cross between 'Samgang' and 'Nagdong', was planted and determined for protein content over three years. Based on the population and a genetic linkage map of 172 markers, QTL analysis was conducted by WinQTLcart 2.5 and QTLMAPPER. Three main-effect QTLs affecting protein content of brown rice were detected from 2004 to 2006 on chromosomes 1 and 11. The qPC11.2 was repeatedly detected across two years. Seven pairs of epistatic loci were identified on eight chromosomes for protein content and collectively explained 39.15% of phenotype variation. These results suggest that epistatic effects might be an even more important component of the genetic basis for protein content and that the segregation of the DH lines for protein content could be largely explained by a few main-effect QTLs and many epistatic loci.
Keywords
protein content; main-effect QTLs; epistasis; QE interactions;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Cho, Y G., M. Y Eun, S. R. McCouch, and Y. A Chae. 1994. The semidwarf gene, sd-l, of rice (Oryza sativa L.). II. Molecular mapping and marker-assisted selection. Theor. Appl. Genet. 89 : 54-59   DOI   ScienceOn
2 Juliano, B. O. 2003. Rice chemistry and quality, 3rd ed., pp. 25-39, Philippine rice research institute, Manila
3 Nakamura, A, S. Komatsu, B. S. Xia, and H. Hirano. 1995. Linkage analysis of the gene loci for seed glutelin (Glu-l), semidwarfism (sd-l) and shattering habit (sh-2) in rice (Oryza sativa L.). Breeding Sci. 45 : 185-188
4 Hu, Z. L., P. Li, M. Q. Zhou, Z. H. Zhang, L. X. Wang, L. H. Zhu, and Y. G. Zhu. 2004. Mapping of quantitative trait loci (QTLs) for rice protein and fat content using doubled haploid lines. Euphytica. 135 : 47-54   DOI   ScienceOn
5 Xing, Y. Z., Y. F. Tan, J. P. Hua, X. L. Sun, C. G. Xu, and Q. F. Zhang. 2002. Characterization of the main effects, epistatic effects and their environmental interactions of QTLs on the genetic basis of yield traits in rice. Thcor. Appl. Genet. 105 : 248-257   DOI
6 Mohan, M., S. Nair, A. Bhagwat, T. G. Krishna, M. Yano, C. R. Bhatia, and T. Sasaki. 1997. Genome mapping, molecular markers and marker-assisted selection in crop plants. Mol. Breed. 3 : 87-103   DOI   ScienceOn
7 Kim, S. M. 2007. Molecular mapping and DNA markerassisted selection of resistance genes to brown planthopper in rice. Ph.D Thesis. Kyungpook National University, Korea
8 Li, Z. K., R. M. Pinson, D. William, A H. Paterson, and J. W. Stansel. 1997. Epistasis for three grain yield components in rice. Genetics. 145 : 453-465   PUBMED
9 Wang, D. L., J. Zhu, Z. K. Li, and A Paterson. 1999. Mapping QTLs with epistatic and QTL x environmental interactions by mixed linear model approaches. Theor. Appl. Genet. 99: 1255-1264   DOI   ScienceOn
10 Qin, Y, S. M. Kim, and J. K. Sohn. 2008. Detection of Main-effect QTLs, Epistatic QTLs and QE Interactions for Grain Appearance of Brown Rice (Oryza sativa L.), J. Crop Sci. Biotech. 11(2) : 151-156
11 Fan, C. C., X. Q. Yu, Y Z. Xing, C. G. Xu, L. J. Luo, and Q. F. Zhang. 2005. The main effects, epistatic effects and environmental interactions of QTLs on the cooking and eating quality of rice in a doubled-haploid line population. Theor. Appl. Genet. 110 : 1445-1452   DOI   ScienceOn
12 Yano, M. and T. Sasaki. 1997. Genetic and molecular dissection of quantitative traits in rice. Plant Mol. Biol. 35 : 145-153   DOI   ScienceOn
13 Yoshida, S., M. Ikegami, J. Kuze, K. Sawada, Z. Hashimoto, T. Ishii, C. Nakamura, and O.Karnihima. 2002. QTL analysis for plant and grain characters of sake-brewing rice using a doubled haploid population. Breeding Sci. 52 : 309-317   DOI   ScienceOn
14 Aluko, G., C. Martinez, J. Tohme, C. Castano, C. Bergman, and J. H. Oard. 2004. QTL mapping of grain quality traits from the interspecific cross Oryza sativa ${\times}$ O. glaberrima. Theor. Appl. Genet. 109 : 630-639   PUBMED
15 Cho, Y. G., H. J. Kang, Y T. Lee, S. R. McCouch, and M. Y Eun. 2006. QTL analysis of rice quality using a recombinant inbred population. J. Agr. Sci, Chungbuk Nat'l Univ. 23 : 147-164