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Extraction Efficiency and Stability of Anthocyanin Pigments in Black Soybean Seed Coat  

Kim, Sun-Young (College of Natural Sciences, Soonchunhyang Univ.)
Ko, Kwang-Oh (College of Natural Sciences, Soonchunhyang Univ.)
Lee, Young-Sang (College of Medical Sciences, Soonchunhyang Univ.)
Kim, Hee-Seon (College of Natural Sciences, Soonchunhyang Univ.)
Kim, Yong-Ho (College of Medical Sciences, Soonchunhyang Univ.)
Publication Information
KOREAN JOURNAL OF CROP SCIENCE / v.53, no.spc, 2008 , pp. 84-88 More about this Journal
Abstract
This study was carried out to develop the methods for efficient extracting and stabilization of anthocyanin pigments in black colored soybean. The optimum solvent concentration for the highest pigment extraction from seed coat of black colored soybean was 60% MeOH with 0.1% HCl in laboratory test. The higher temperature of the solvent until $60^{\circ}C$ led to better extraction efficiency of anthocyanin. The anthocyanin extracts maintained almost its stability in strong acidity area whereas it destroyed markedly over pH 4. Also anthocyanin extracts was stable for a while under weak light condition, but it showed rapid degradation of pigment color and markedly decreased HPLC value for anthocyanin content after one month storage days. In case storage temperature and instrument, glass vessel on low temperature ($4^{\circ}C$) was good storage condition among some treatments. And C3G was more stable than other pigments.
Keywords
soybean; anthocyanin; extraction efficiency; stability;
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1 Eskin, N. A. M. 1979. Plant pigments, flavors and textures. in The chemistry and biochemistry of selected compounds. Academic Press. New York. p. 228
2 Francis, F. J. 1989. Food colorants: Anthocyanins. Crit. Rev. Food Sci. Nutr. 28 : 273-314
3 Havsteen, B. 1983. Flavonoids : a class of natural products of high pharmacological potency. Biochem. Pharmacol. 32 : 1141-1145
4 Kim, S. L., J. J. Hwang, J. Song, J. C. Song, and K. H. Jung. 2000. Extraction, purification and quantification of anthocyanins in colored rice, black soybean, and black waxy corn. Korean J. Breed. 32(2) : 146-152
5 Kim, Y. H., H. T. Yun, K. Y. Park, and S. D. Kim. 1997. Extraction and separation of anthocyanins in black soybean. RDA J. Crop. Sci. 39 : 35-38
6 Martin, W. and G. Harzdina. 1978. High-pressure liquid chromatographic seperation of 3-glucoside, 3,5-diglucosides, 3-(6-O-p- coumaryl) glucosides and 3-(6-O-p-coumarylglucoside)-5-glucosides of anthocyanidins. J. Chromatography 155 : 389-398
7 Choung, M. G., I. Y Baek, and S. T Kang. 2001. Isolation and determination of anthocyanins in seed coats of black soybean. J. Agr. Food Chem. 49 : 5848-5851
8 Hayes, R. E., G. N. Bookwalter, and E. B. Bagley. 1997. Antioxidant activity of soybean flour and derivatives - A review. J. Food Sci. 42(6) : 1527-1531
9 Jackman, R. L., R. Y. Yada, M. A. Tung, and R. A. Speers. 1987. Anthocyanins as food colorants - a review. J. Food Biochem. 11 : 201-274
10 Kenedy, A. R. 1995. The evidence for soybean products as cancer preventive agents. J. Nutr. 125 : 733S-743S
11 Palamidis, N. M. 1975. Stability of grape anthocyanin in a carbonated beverage. J. Food Sci. 40 : 1047-1049
12 Yoshikura, K. and Y. Hamaguchi. 1969. Anthocyanins of the black soybean. Eiyo To Shokuryo. 22 : 367
13 Aleksandrova, V. G. and O. G. Aleksandrova. 1943. The distribution of pigments in the testa of some varieties of soybean. Bull. Appl. Bot. Genet. Plant Breed. U.S.S.R. 3, 4 (CA 29, 5149)
14 Markakis, P. 1974. Anthocyanins and their stability in foods. Crit. Rev. Food Technol. 4 : 437-456
15 Messina, M., C. Gardner, and S. Barnes. 2002. Gaining insight into the health effects of soy but a long way still to go: Commentary on the fourth international symposium on the role of soy in preventing and treating chronic disease. J. Nutrition 132 : 547S-551S
16 Choi, H. C. 1996. Develolment and industrial utilization of natural pigments from colored rices. G7 final report. Ministry of Science & Technology
17 Kim, Y. H., H. T. Yun, and K. Y. Park. 2004. 12. Biological effects of black colored soybean. Plant Resources 7(3) : 195-199