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Antioxidant Activity and Cytotoxicity on Human Cancer Cells of Anthocyanin Extracted from Black Soybean  

Kim, Yong-Ho (College of Medical Sciences, Soonchunhyang Univ.)
Kim, Dong-Seon (Unigen, INC.)
Woo, Sung-Sick (Unigen, INC.)
Kim, Hyun-Hee (College of Natural Sciences, Soonchunhyang Univ.)
Lee, Young-Sang (College of Medical Sciences, Soonchunhyang Univ.)
Kim, Hee-Seon (College of Natural Sciences, Soonchunhyang Univ.)
Ko, Kwang-Oh (College of Natural Sciences, Soonchunhyang Univ.)
Lee, Seuk-Ki (National Institute of Crop Science, RDA)
Publication Information
KOREAN JOURNAL OF CROP SCIENCE / v.53, no.4, 2008 , pp. 407-412 More about this Journal
Abstract
Anthocyanin pigments in soybean seed coat were D3G (Delphinidin-3-glucoside), C3G (Cyanidin-3- glucoside) and Pt3G (Petunidin-3-glucoside), which have been known potential roles in the prevention and treatment of chronic diseases. Anthocyanin contents in seed coat of blck soybean were significantly different according to soybean variety, C3G content showed the highest value in all materials and its variation was also wide. Antioxidant activity of each pigment was analyzed by DPPH and TEAC methods in which D3G and C3G showed high activity. And this study was carried out to investigate the effects of anthocyanin to human cancer cells. Cytotoxity were analyzed by MTT assay after anthocyanin pigments treated on leukemia (Jurkat T) and adenocarsinoma (MCF-7) cells. It showed decrement of cell numbers as anthocyanin concentration is increasing. ${EC}_50$ range of anthocyanin concentrations were $100{\sim}250\;ug/mL$ and $100{\sim}250\;ug/mL$ in Jurkat T and MCF-7 cell, respectively. D3G showed higher cytotoxicity than other pigments in Jurkat T cell whereas activity of C3G was high in MCF-7 cell. It is believed that supplementation of human diets with soybean anthocyanin markedly reduces human cancer mortality rates.
Keywords
soybean; anthocyanin; antioxidant activity; cytotoxity; cancer;
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