Browse > Article

Natural Photodynamic Activity of 5-Aminolevulinic Acid Produced by E. coli Overexpressing ALA Synthase from Bradyrhizobium japonicum  

Chon Sang-Uk (Callus Co. Ltd., TBI Center, Gwangju Institute of Science and Technology)
Jung Sun-Yo (School of Biological Sciences and Biotechnology, Kyungbook National University)
Boo Hee-Ock (Phyto M&F, BIC, Dongshin University)
Han Seung-Kwan (Center for EM Research and Development, Jeonju University)
Publication Information
KOREAN JOURNAL OF CROP SCIENCE / v.51, no.4, 2006 , pp. 356-361 More about this Journal
Abstract
The present study was conducted to determine plant growth and physiological responses of corn, barnyardgrass, and soybean to ALA (5-aminolevulinic acid). ALA effect on early seedling growth of test plants was greatly concentration dependant, suggesting that it inhibits at higher concentrations. No significant difference in herbicidal activity of two types of ALA on plant height and weight of test plants was observed. Barnyardgrass was the most sensitive to ALA and followed by corn and soybean, indicating that both crop plants were less affected by ALA concentration as well as different growth stages than barnyardgrass. Greatly reduced chlorophyll contents from leaves of three plant species were observed with increasing of ALA concentration. Compared with untreated controls, higher amounts of three tetrapyrroles were detected from three crop plants, indicating more accumulation in ALA-treated plants. The differential selectivity among plant species would be explained with the differences in tetrapyrrole accumulating capabilities, the susceptibility of various greening groups of plant species to the accumulation of various tetrapyrroles, and their metabolism in various plant tissues. The results indicate that negative biological potential of ALA exhibited differently on plant species, and that the photodynamic herbicidal activity against susceptible plants highly correlated with the extent of tetrapyrrole accumulation by the species.
Keywords
5-aminolevulinic acid; herbicidal potential; tetrapyrrole accumulation; eco-friendly weed management;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Papenbrock, J. and B. Grimm. 2001. Regulatory network of tetrapyrrole biosynthesis-studies of intracellular signaling involvedin metabolicand developmental control of plastids. Planta 213 : 667-681   DOI
2 Weinstein, J. D. and S. I. Beale. 1983. Separate physiological roles and sub-cellular compartments for two tetrapyrrole biosynthetic pathways in Euglena gracilis. J. Biol. Chem. 258 : 6799-6807
3 Rebeiz, C. A., A. Motazer-Zouhoor, J. M. Mayasich, B. C. Tripathy, S. M. Wu, and C. C. Bebiz. 1988a. Photodynamic herbicides. Recent developments and molecular basis of selectivity. Crit. Rev. Plant Sci. 6 : 385-486   DOI
4 Duke, S. O., J. Lydon, J. M. Becerril, T.D. Sherman, L. P. Lehnen, and H. Matsumoto. 1991. Protoporphyrinogen oxidase-inhibiting herbicides. Weed Sci. 39 : 465-473
5 Beale, S. I. and P. A. Castelfranco. 1974. The biosynthesis of ${\delta}-aminolevulinic$ acid in higher plants. II. Formation of $^{14}C-{\delta}-aminolevulinic$ acid from labeled precursors in greening plant tissues. Plant Physiol. 53 : 297-303   DOI   ScienceOn
6 Rebeiz, C. A., K. N. Reddy, and U. B. Nandilhalli, 1990. Tetrapyrrole-dependent photodynamic herbicide. Photochem. Photobiol. 52 : 1099-1117   DOI
7 Askira, Y., B. Rubin, and H. D. Rabinowitch. 1991. Differential response to the herbicidal activity of ${\delta}-aminolevulinic$ acid in plants with high and low SOD activity. Free Rad. Res. Comms. 12-13 : 837-843
8 Chon, S. U., Y. I. Kuk, and J. O. Guh. 2004. Microbiological production and herbicidal mechanism of 5-aminolevulinic acid as tetrapyrrole-dependent photodynamic herbicide. Korean J. Weed Science 24 : 161-172
9 Choi, C., B. S. Hong, H. C. Sung, H. S. Lee, and J. H. Kim. 1999. Optimization of extracellular 5-aminolevulinic acid production from Escherichia coli transformed with ALA synthase gene for Bradyrhizobium japonicum. Biotech. Letters 21 : 551-554   DOI   ScienceOn
10 Lichtenthaler, H. K. 1987. Chlorophylls and carotenoids: pigments of photosynthetic biomembranes. Methods in Enzymology 148 : 350-382   DOI
11 Boger, P. and K. Wakabayashi. 1999. Peroxidizing herbicides. Springer, Berlin, Heidelberg
12 Beale, S. I. 1990. Biosynthesis of the tetrapyrrole pigment precursor, ${\delta}-aminolevulinic$ acid, from glutamate. Plant Physiol. 93 : 1273-1279   DOI   ScienceOn
13 Chon S. U. 2003. Herbicidal activity of ${\delta}-aminolevulinic$ acid on several plants as affected by application methods. Korean J. Crop Sci. 48 : 50-55
14 Kuk, Y. I., G. S. Lim, S. U. Chon, T. E. Hwang, and J. O. Guh. 2003. Effect of 5-Aminolevulinic acid on growth and Inhibition of Various Plant Species. Kor. J. Crop Sci. 48 : 127-133
15 Wettstein, D. von, S. Gough, and C.G. Kannangara. 1995. Chlorophyll biosynthesis. Plant Cell 7 : 1039-1057   DOI   ScienceOn
16 Sasaki, K., T. Tanaka, Y. Nishizawa, and M. Hayashi. 1990. Production of a herbicide, 5-aminolevulinic acid, by Rhodobacter sphaeroides using the effluent waste from an anaerobic digestor. Appl. Microbiol. Biotechnol. 32 : 727-731   DOI
17 Scalla, R. and M. Matringe. 1994. Inhibitors of protoporphyrinogen oxidase as herbicides: Diphenyl ethers and related photobleaching molecules. Rev. Weed Sci. 6 : 103-132
18 Schuimaker, J. J., P. Baas, L. M. van Leengoed, F.W. van der Meulen, W. M. Star, and N. van Zandwijk. 1999. Photodynamic therapy: a promising new modality for treatment of cancer. J. Photochem. Photobiol. 34 : 3-12
19 Mock, H. P., U. Keetman, and B. Grimm. 2002. Photosensitising tetrapyrroles induce antioxidative and pathogen defense responses in plants, in: D. Inze, M. van Montagu (Eds.). Oxidative Stress in Plants. Taylor and Francis, London, NY. pp.155-170
20 Rebeiz, C. A., A. Montazer-Zouhoor, H. J. Jopen, and S. M. Wu. 1984. Photodynamic herbicides: Concept and phenomenology. Enzyme Microb. Technol. 6 : 390-401   DOI   ScienceOn
21 SAS (Statistical Analysis System). 2000. SAS/STAT user's guide. Version 7. Cary, NC: Statistical Analysis Systems Institute. Electronic Version
22 Menon, I. A., S. D. Persad, and H. F. Haberman. 1989. A comparison of the phytotoxicity of protoporphyrin, coproporphyrin, and uroporphyrin using a cellular system in vitro. Clin. Biochem. 22 : 197-200   DOI   ScienceOn
23 Tripathy, B. C. and N. Chakraborty. 1991. 5-aminolevulinic acid induced photodynamic damage of the photosynthetic electron transport chain of cucumber (Cucumis sativus L.) cotyledons. Plant Physiol. 96 : 761-767   DOI   ScienceOn
24 Avissar, Y. J., J. G. Ormerod, and S. I. Beale. 1989. Distribution of 5-aminolevulinic acid acid biosynthetic pathways among phototrophic bacterial groups. Arch. Microbiol. 151 : 513-519   DOI
25 Beale, S. I. and J. D. Weinstein. 1990. Tetrapyrrole metabolism in photosynthetic organisms. In Biosynthesis of Heme and Chlorophylls (Ed.), Dailey, H.A. pp. 287-391. McGraw-hill, New York
26 Rebeiz, C. A., J. A. Juvik, and C. C. Rebeiz. 1988b. Photodynamic insecticides I. Concept and phenomenology. Pesticide Biochem. Physiol. 30: 11-27   DOI