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Plant Regeneration from Sliced Mature Embryo Fragments of Wheat Cultivars  

Kim Kyung-Hee (Department of Plant Biotechnology, Dongguk University)
Kang Moon-Seok (National Institute of Crop Science, RDA)
Kwon Young-Up (National Institute of Crop Science, RDA)
Lee Sang-Kyu (Department of Plant Biotechnology, Dongguk University)
Moon Jung-Hun (Department of Plant Biotechnology, Dongguk University)
Han Sinae (Department of Plant Biotechnology, Dongguk University)
Oh Poo-Reum (Department of Plant Biotechnology, Dongguk University)
Lee Byung-Moo (Department of Plant Biotechnology, Dongguk University)
Publication Information
KOREAN JOURNAL OF CROP SCIENCE / v.50, no.5, 2005 , pp. 356-360 More about this Journal
Abstract
Mature embryos were aseptically excised with a scalpel and sliced in fragments measuring 0.5 mm in diameter (sliced mature embryo fragment; 4 ${\~}$ 5 fragments/one embryo). Sliced mature embryo fragments of six wheat cultivars were cultured to develop an efficient method of callus induction and plant regeneration. Callus derived from sliced mature embryo fragments showed a good capacity to embryogenesis and regeneration. Furthermore sliced mature embryo fragments decreased contamination from fungi and bacteria. The high efficiency of callus induction were obtained Keumkangmil and Bob­white. For plant regeneration, selected embryogenic calli were transferred to two types regeneration media. An average number of green spots per callus was 4 to 5 in regeneration media after about one week. Percentage of plant regeneration showed high in regeneration medium containing 0.1 mg/l 2,4-D and 5 mg/l zeatin. Especially, Keumkangmil ($27.5\%$) and Bobwhite ($33.3\%$) showed high regeneration efficiency. This regeneration system from sliced mature embryo fragments may provide an effective and convenient explant for plant transformation studies.
Keywords
wheat; sliced mature embryo fragments; callus induction; plant regeneration;
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1 Bhaskaran, S. and R. H. Smith. 1990. Regeneration in cereal tissue culture: a review. Crop Sci. 30 : 1328-1336   DOI
2 Becker, D., R. Brettschneider, and H. Lorz, 1994. Fertile transgenic wheat from microprojectile bombardment of scutellar tissue. Plant J. 5 : 299-307   DOI   ScienceOn
3 Fennell, S., N. Bohorova, M. Van Ginkel, J. Crossa, and D. Hoisington. 1996. plant regeneration from immature embryos of 48 elite bread wheats. Theor. Appl. Genet. 92: 163-169   DOI   ScienceOn
4 Mc Kinnon, C., G. Gunderson, and M. W. Nabors. 1987. High efficiency plant regeneration by somatic embryogenesis from callus of mature embryo explants of bread wheat (Triticum aestivum) and grain sorghum (Sorghum bicolor). In Vitro Cell. Dev. Biol. 23 : 443-448   DOI
5 Mendoza, M. G. and H. F. Kaeppler. 2002. Auxin and sugar effects on callus induction and plant regeneration frequencies from mature embryos of wheat (Triticum aestivum L.). In Vitro Cell. Dev. Biol. 38 : 39-45   DOI   ScienceOn
6 Moon, J. H., S. K. Lee, K. H. Kim, M.S. Kang, H.Y Heo, Y. U. Kwon, J. H. Nam, Y. W. Seo, and B. M. Lee. 2003. Efficient callus induction and plant regeneration from immature and mature embryo culture of Korean wheat genotypes. Korean J. Crop Sci. 48(1): 38-43
7 Ozias-Akins, P. and I. K. Vasil. 1982. Plant regeneration from cultured immature embryos and inflorescences of Triticum aestivum (L. wheat) : evidence for somatic embryogenesis, Protoplasma. 110 : 95-105   DOI
8 Redway, F. A., V. Vasil, D. Lu, and I. K. Vasil. 1990. Identification of callus types for long-term maintenance and regeneration from commercial cultivars of wheat (Triticum aestivum L.). Theor. Appl. Genet. 79 : 609-617
9 Sharma, V. K., A. Rhao, A. Varshney, and S. L. Kothari. 1995. Comparison of developmental stage of inflorescence for high frequency plant regeneration in Triticum aestivum L. and Triticum durum L. Desf. Plant Cell Rep. 15 : 227-231   DOI
10 Varshney, A., T. Kant, K. V. Sharma, A. Rao, and S. C. Kothari. 1996. High frequency plant regeneration from immature embryo cultures of Triticum aestivum and T. durum. Cereal Res. Commum. 24 : 409-413
11 Vasil, V., F. Redway, and I. K. Vasil. 1990. Regeneration of plants from embryogenic suspension culture protoplasts of wheat (Triticum aestivum L.). Bio/Tcchnology, 8 : 429-434   DOI
12 Viertel, K. and D. Hess. 1996. Shoot tips of wheat an alternative source for regenerable embryogenic callus cultures. Plant Cell Tiss. Org. Cult. 44 : 183-188   DOI   ScienceOn
13 Altpeter, F., V. Vasil, V. Srivastava, E. Stager, and I. K. Vasil. 1996. Accelerated production of transgenic wheat (Triticum aestivum L.) plants. Plant Cell Rep. 16: 12-17   DOI   ScienceOn
14 Abdrabou, R. T. and R. A. K. Moustafa. 1993. Effect of 2,4-D concentrations and levels of sucrose on callus induction and plantlet formation in two wheat cultivars. Annuals of Agricultural Science Cario. No.1 (special issue). 41-46
15 Ben Amer, I. M. and A. Bomer. 1997. The relationship between green spot initiation and plantlet regeneration of wheat (Triticum aestivum L.) callus grown under short-term conditions. Plant Cell Tiss. Organ Cult. 50 : 67-69   DOI   ScienceOn
16 Elena, E. B. and H. D. Ginzo. 1988. Effect of auxin levels on shoot formation with different embryo tissues from a cultivar an a commercial hybrid of wheat (Triticum aestivum L.). J. Plant Physiol. 132 : 600-603   DOI
17 La Rue, C. D. 1949. Cultures on the endosperm of maize. Am. J. Botany. 34 : 585-586
18 Liu, W., M. Y. Zheng, and C. F. Konzak. 2002. Improving green plant production via isolated microspore culture in bread wheat (Triticum aestivum L.). Plant Cell Reports. 20 : 821-824   DOI   ScienceOn
19 Delporte, F., O. Mostade, and J. M. Jacquemin. 2001. Plant regeneration through callus initiation from thin mature embryo fragments of wheat. Plant Cell Tiss. Organ Cult. 67 : 73-80   DOI   ScienceOn
20 Shimada, T., T. Sasakuma, and K. Tsunewaki. 1969. In vitro culture of wheat tissues. I. callus formation, organ redifferentiation and single cell culture. Can. J. Genet. Cytol. 11 : 294-304   DOI
21 Ozias-Akins, P. and I. K. Vasil. 1983. Improved efficiency and normalization of somatic embryogenesis in Triticum aestivum (wheat). Protoplasma. 117: 40-4   DOI
22 He, D. G, Y. M. Yang, and K. J. Scott. 1992. Plant regeneration from protoplasts of wheat (Triticum aestivum cv Hartog). Plant Cell Rep. 11 : 16-19   DOI   ScienceOn
23 Marsolais, A. A. and K. J. Kasha. 1985. Callus induction from barley microspores: the role of sucrose and auxin in a barley anther culture medium. Can J Bot. 63 : 2209-2212   DOI
24 Li, Z. Y., G. M. Xia, and H. M. Chen. 1992a. Somatic embryogenesis and plant regeneration from protoplast isolated from embryogenic cell suspension of wheat (Triticum aestivum L.). Plant Cell Tiss. Org. Cult. 28 : 79-85   DOI
25 Chawala, H. S. and G. Wenzel. 1987. Regeneration potential of callus from wheat and barley. Archiv fur Zuchtungsforschung. Bockhom, 8059 Grunbach, German Federal Republic. 17(6) : 337-343
26 Eapen, S. and Rao. 1985. Factors controlling callus induction, growth and plant regeneration in bread wheat (Triticum aestivum L.). Proc. Indian Acad. Sci. Plant Sci 94 : 33-40
27 Gamborg, O. L. and D. E. Eveleigh. 1968. Culture methods and detection of glucanases in suspension cultures of wheat and barley. Can. J. Biochem. 46: 417-421   DOI
28 Mohammad, I. S., J. Mussarat, and I. Ihsan. 2003. In vitro callus induction, its proliferation and regeneration in seed explants of wheat (Triticum aestivum L.) Var. Lu-26S. Par. J. Bot. 35(2) : 209-217
29 Machii, H., H. Hizuno, T. Hirabayashi, H. Li, and T. Hagio. 1998. Screening wheat genotypes for high callus induction and regeneration capability from anther and immature embryo cultures. Plant Cell Tiss. Organ Cult. 53 : 67-74   DOI   ScienceOn
30 Mejza, S. J., V. Morgant, D. E. Di Bona, and J. R. Wong. 1993. Plant regeneration from isolated microspores of Triticum aestivum. Plant Cell Rep. 12 : 149-153
31 Maddock, S. E., V. A. Lancaster, R. Risiott, and J. Franklin. 1983. Plant regeneration from cultured immature embryos and inflorescences 01'25 cultivars of wheat (Triticum aestivum). J. Exp. Bot. 34 : 915-926   DOI
32 Nehra, N. S., R. N. Chibbar, N. Leung, K. Caswell, C. Mallard, L. Steinhauer, M. Baga, and K. Kartha. 1994. Self-fertile transgenic wheat plant regenerated from isolated scutellar tissues following microsprojectile bombardment with two distinct gene construct. Plant J. 5 : 285-297   DOI   ScienceOn
33 Liang, G. H., A. Xu, and H. Tang. 1987. Direct regeneration of wheat haploids via anther culture. Crop Sci. 27 : 336-339   DOI
34 Chu, C. C. and R. D. Gill. 1988. An improved anther culture method to obtain higher frequencies of pollen embryos in wheat (Triticum aestivum L.). Plant Sci. 55 : 175-181   DOI   ScienceOn
35 Zhou, H., Y. Zheng, and C. F. Konsak. 1991. Osmotic potential of media affecting green plant percentage in wheat anther culture. Plant Cell Rep. 10 : 63-66   DOI   ScienceOn
36 Hu, T. and K. J. Kasha. 1997. Improvement of isolated microspore culture of wheat (Triticum aestivum L.) through ovary co-culture. Plant Cell Reports. 16: 520-525   DOI
37 Ball, S. T., H. Zhou, and C. F. Konzak. 1993. Influence of 2,4-D, IAA, and duration of callus induction in anther culture of spring wheat. Plant Sci. 90 : 195-200   DOI   ScienceOn
38 Barro, F., A. Martin, P.A. Lazzeri, and P. Barcelo. 1999. Medium optimization for efficient somatic embryogenesis and plant regeneration from immature inflorescences and immature scutella of elite cultivars of wheat, barley, and tritodeum, Eupytica. 108: 161-167   DOI
39 Orshinsky, B. R. and R. S. Sadasivaiah. 1997. Effect of plant growth conditions, plating density, and genotype on the anther culture response of soft white spring wheat hybrids. Plant Cell Reports. 16 : 758-762   DOI   ScienceOn
40 Zheng, M. Y. and C. F. Konzak. 1999. Effect of 2,4-dichlorophe-noxyacetic acid on callus induction and plant regeneration in anther culture of wheat (Triticum aestivum L.). Plant Cell Reports. 19 : 69-73   DOI   ScienceOn
41 Murashige, T. and F. Skoog. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15 : 473-497   DOI
42 Brisibe, E. A., A. Gajdosova, A. Olesen, and S. B. Andersen. 2000. Cytodifferentitation and transformation of embryogenic callus lines derived from anther culture of wheat. Journal of Experimental Botany. 51(343) : 187-196   DOI   ScienceOn
43 Hess, J. R. and J. G. Carman. 1988. Embryogenic competence of immature embryos: genotype, donor plant environment, and endogenous hormone levels. Crop Sci. 38 : 249-253   DOI   ScienceOn
44 Li, Z. Y., G. M. Xia, H. M. Chen, and G. Q. Guo. 1992b. Plant regeneration from protoplast derived from embryogenic suspension culture of wheat (Triticum aestivum L.). J Plant Physiol. 139: 714-718   DOI
45 Last, D. and R. I. Brettell. 1990. Embryo yield in anther culture is influenced by the choice of sugar in the culture medium. Plant Cell Rep. 9 : 14-16