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Antioxidative Activities in Rice Leaves Exposed to Ozone  

Lee, Su-Yeon (Department of Plant Resources Science, Hankyong National University)
Sol Yun (Department of Plant Resources Science, Hankyong National University)
Lim, Hyo-Jin (Department of Plant Resources Science, Hankyong National University)
Sung, Jwa-Kyung (Department of Agronomy, Chungbuk National University)
Hwang, Seon-Woong (Division of Plant Nutrition, NIAST, RD)
Huh, Kwang-Woon (Institute of Ecological Phytochemistry, HanKyong National University)
Kim, Tae-Wan (Department of Plant Resources Science, Hankyong National University)
Publication Information
KOREAN JOURNAL OF CROP SCIENCE / v.49, no.2, 2004 , pp. 110-115 More about this Journal
Abstract
Rice (Oryza sativa L.) plants were cultivated to examine changes in antioxidative defence mechanism induced by elevated ozone levels. Catalase activities in tolerant Jinpumbyeo and susceptible Chucheongbyeo under ozone fumigation were reduced at 5 hrs and 3 hrs after ozone fumigation, respectively. With the increased ozone supply, peroxidase activity in Jinpumbyeo was steadily enhanced whereas in Chucheongbyeo it was not changed. Four SOD-isozymes were detected by NBT staining of native-PAGE. Two isozymes of them were obviously induced by ozone supply, particularly in Jinpumbyeo. The continuous ozone fumigation increased remarkably putrescine levels in leaves whereas it did not affect the levels of spermidine and spermine. In this study, it was implied that ozone in cell inhibits strongly diamine oxidase and thus promotes ethylene biosynthesis which will cause the senescence in rice plants.
Keywords
rice; superoxide dismutase; catalase; peroxidase; polyamine;
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  • Reference
1 Aebi, H. 1974. Catalases, in: H. U. Bergmeyer(Ed.)Methods of enzymatic analysis, Academic Press, New York, Vol. 2, pp 673-684
2 Beauchamp, C. O. and I. Fridovich. 1971. Superoxide dismutase: improved assays and an assay applicable to acrylamide gels, Anal. Biochem. 44 : 276-287   DOI   ScienceOn
3 Bowler C, W. Van Camp, M. Van Montagu and D. Inze. 1994. Superoxide dismutase in plants. CRC Crit. Rev. Plant. Sci. 13 : 199-218   DOI   ScienceOn
4 Bradford, M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Anal. Biochem. 72: 248-254   DOI   PUBMED   ScienceOn
5 Feurstein, B. G. and L. G. Marton. 1989. Specificity and binding in polyamine: nucleic acid interactions, in: U. Bachrach, Y.M. Heimer (Eds.). The Physiology of Polyamines. vol. 1. CRC Press. Boca Raton. FL. pp. 109-120
6 Van Camp, w., H. Willekens, C. Bowler, M. Van Montague, and K. Inze. 1994. Elevated levels of superoxide dismutase protect transgenic plants against ozone damage. Bio/Technology 12: 165-168   DOI   ScienceOn
7 Bowler, C., M. Van Montagu, and D. Inze. 1992 Superoxide dismutase and stress tolerance. Annu. Rev. Plant Physiol. Plant Mol. BioI. 43 : 83-116   DOI   ScienceOn
8 Winston, G. W. 1990. Physiochemical basis for free radical formation in cells: production and defenses. In Alscher, R. G., Cumming, J. R (Eds.). Stress Responses in Plants: Adaption and Acclimation Mechanisms. Wiley-Liss. Inc. New York. pp. 57-86
9 Carley, E., R. A. Wolosiuk, and C. M. Hertig. 1983. Regulation of the activation of chloroplast fructose-l,6-bis phosphatase (E.C.3.1.3.11). Inhibition by spermidine and spermine. Biochern. Biophys. Res. Commun. 115 : 707-710   DOI   ScienceOn
10 Perl-Treves, R. and E. Galun. 1991. The tomato Cu/Zn superoxide dismutase genes are developmentally regulated and respond to light and stress. Plant Mol. BioI. 17 : 745-760   DOI   ScienceOn
11 Dwivedi, S., M. Kar, and D. Misra. 1979. Biochemical changes in excised leaves of Oryza sativa subjected to water stress. Physiol. Plant. 45 : 35-40.   DOI
12 Peter, J. L., F. J. Castillo, and R. L. Heath. 1989. Alteration of extracellular enzymes in Pinto bean leaves upon exposure to air pollutants, ozone and sulfur dioxide. Plant Physiol. 89 : 159-164
13 Gaspar, T. H., C. Penel, D. Hagega, and H. Greppin. 1991. Peroxidases in plant growth, differentiation and development processes. In: J. Lobarzewski, H. Greppin, C. Penel, & T. H. Gaspar, eds, Biochemical Molecular and physiological Aspects of Plant Peroxidases. University de Geneve, Switzerland, pp. 249-280
14 Putter, J. Peroxidase, in: H. U. Bergmeyer (Ed.). 1974. Methods of enzymatic analysis. vol. 2. Academic Press. NY. pp. 685-690
15 Jacob, S. T. and D.A. Stetler. 1989. Polyamines, and RNA synthesis, in: U. achrach, Q.M. Heimer (Eds.). The Physiology of Polyamines. vol. 1.CRC Press. Boca Raton, FL. pp. 133-140
16 Takeda, Y., K. Samejina, K. Nagano, M. Watanabe, H. Sugeta, and Y. Kyogoku. 1983. Determination of protonation sites in thermospermine and in other polyamines by $^{15}N\;and\;^{13}C$ nuclearmagnetic resonance spectroscopy. Eur. J. Biochem. 130 : 383-386   DOI   ScienceOn
17 Elstner, E. F. 1982. Oxygen activation and oxygen toxicity. Ann. Rev. Plant Physiol. 33 : 73-96   DOI   ScienceOn
18 Tadolini, B., L. Cabrini, E. Varani, and A. M. Sechi. 1985. Spermine binding and aggregation of vesicles of different lipid composition. BioI. Amines 3 : 87-92
19 Malhotra, S. S. and A. A. Khan. 1984. Biochemical and physiological impact ofmajor pollutants. In : Treshow M., (Ed), Air Pollution and Plant Life. John Wiley & Sons, Now York, pp. 113-157
20 Bender, J., H. J. Wegner, U. Wegner, and H. J. J ger. 1994. Response of cellular antioxidants to ozone in wheat flag leaves at different stages of lant development. Environ. 84 : 15-21
21 Castillo, F. J. and H. Greppin. 1988. Extracelllular ascorbic acid and enzyme activities related to ascorbic acid metabolism in Sedum album L. leaves after ozone exposure. Environ. Exp. Botany 28: 231-238   DOI   ScienceOn
22 Brunsch$\ddot{o}$n Harti, S., A. Fangmeier, and H. J. Jager. 1995. Effects of ethylenediurea and ozone on the antioxidative systems in beans (Phaseolus vulgaris L.). Environ. Pollution 90 : 95-103   DOI   ScienceOn
23 Hill, A. C., M. R. Pack, M. Treshow, R. J. Downs, and L. G. Thranstrum. 1961. Plant injury induced by ozone. Phytopathology 51 : 356-363
24 Lee, E. H., J. A. Jersey, C. Gifford, and J. Bennett. 1984. Differential ozone tolerance in soubean and snapbeans: Analysis of ascorbic acid in $O_3$-susceptible and $O_3$-resistant cultivars by high-performance liquid chromatography. Environ. EXp. Botany 24: 331-335   DOI   ScienceOn
25 Heby, O. 1981. Role of polyamines in the control of cell proliferation and differentiation. Differentiation 19 : 1-12   DOI   PUBMED
26 Willekens, H, W.Van Camp, M. Van Montagu, D. Inze, C. Langerbelts, and H. Jr. Sandermann. 1994. Ozone, sulfur dioxide and UV-B radiation have similar effects on mRNA accumulation of antioxidant genes in Nicotiana plumbaginifolia L. Plant Physiol. 106 : 1007-1014   DOI
27 Marton, L. and D. Morris. 1987. Molecular and cellular functions of the polyamines, in: P.P. McCann, A. Pegg, A. Sjoerdsma (Eds.). Inhibition of Polyamine Metabolism. Academic Press. San Diego. CA. pp. 79-105
28 Fridovich, I. 1986. Superoxide dismutases, in: Advances in Enzymology and Related Areas of Molecular Biology, Wiley, New York, pp. 5861-5897
29 Oeer, T. and A. Polle. 1994. The influence of apoplastic ascorbate on the activities of cell-wall associated peroxidases and NADH-oxidases in needles of Norway spruce (Picea abies L.). Plant Cell Physiol. 35 : 1231-1238   DOI
30 Redmond, J. W. and A. Tseng. 1979. High pressure liquid chromatographic determination of putrescine, cadaverine, spermidine and spermine. J. hromatogr. 170 : 479-481   DOI   ScienceOn