Browse > Article

Radical Scavenging Activity and Cytotoxicity of Maysin(C-glycosylflavone) isolated from Silks of Zea mays L.  

Kim, Sun-Lim (National Crop Experiment Station, R.D.A.,)
Snook, Maurice-E (Phytochemical Research Unit, Russell Research Center, ARS-USDA)
Lee, Jong-Ock (Korea Research Institute of Chemical Technology)
Publication Information
KOREAN JOURNAL OF CROP SCIENCE / v.48, no.5, 2003 , pp. 392-396 More about this Journal
Abstract
Maysin, a C-glycosylflavone, was isolated from the silks of maize, Zea mays L. The ESI mass spectrum indicates that molecular weight of maysin is $577\textrm{M}^+$m/z, and the ether-linked sugar is rhamnose, $431\textrm{M}^+$m/z (MW$^{+}$-146). The DPPH (1,1-Diphenyl-2-picrylhydrazyl) radical scavenging activity of maysin was higher than that of rutin. However, as compared with its aglycon luteolin, maysin showed the relatively moderate DPPH scavenging activity mainly due to the glycosylation of two sugars moieties, keto-fucose and rhamnose. In the in vitro cytotoxicity test against the five human tumor cell lines such as lung (A549), ovarian (SK-OV-3), melanoma (SK-MEL-2), central nerve system (XF-489), and colon (HCT-15), maysin exhibited the relatively weaker activities than cisplatin. The $\textrm{ED}_{50}$ values of maysin were 62.24, 43.18, 16.83, 37.22, and 32.09/$m\ell$, respectively. Result suggests that maysin is a potential cytotoxicity compound, particularly for human colon, central nerve system, and melanoma tumors.s.
Keywords
corn silk; maysin; DPPH radical scavenging activity; cytotoxicity;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Blois, M.S. 1958. Antioxidant detennination by the use of a stable free radical. Nature 26 : 1199-1200
2 Byrne, P.E., L.L. Darrah, M.E. Snook, B.R. Wiseman, N.W. Widstrom, D.J. Moellenbeck, and B.D. Barry, 1996. Maize silkbrowning, maysin content, and antibiosis to the corn earwonn, Helicoverpa zea (Boddie). Maydica 41: 13-18
3 Cai, Q., R.O. Rahn, and R. Zhang. 1997. Dietary flavonoids, quercetin, luteolin and genistein, reduce oxidative DNA damage and lipid peroxidation and quench free radicals. Cancer Letters 119: 99-107   DOI   ScienceOn
4 Doan, D.D., N.H. Nguyen, H.K. Doan,T.L. Nguyen,T.S. Phan, van D. Nguyyen, G. Magnus, J. Rolf, L. Gerd, and E.S. Nils. 1992. Studies on the individual and combined diuretic effects of four Vietnamese traditional herbal remedies (Zea mays, Imperata cylindrical, Plantago major and Orthosiphon stamineus). Journal of Ethnopharmacology 36: 225-231   DOI   ScienceOn
5 Martin, N., C. Pantoja, L. Chiang, C. Bardisa, C. Araya, and R. Roman. 1991. Hemodynamic effects of a boiling water dialysate of maize silk in normotensive anaesthetized dogs. Journal of Ethnopharmacology 31 : 259-262   DOI   ScienceOn
6 Barry, M.A., C.A. Behnke, and A. Eastman. 1990. Activation of programmed cell death (apoptosis) by cisplatin, other anticancer drugs, toxins and hyperthermia. Biochem. Pharmcol. 40: 2353-2362   DOI   ScienceOn
7 Kim, S.L, B.H. Choi, S.U. Park, and H.G. Moon. 1996. Functional ingredients and their variation. Korean J. Crop Sci. 41(S) : 46-68
8 Monks, A, D.A. R. Scudiero, R.H. Skehan, R.H. Shoemaker, K.D. Paull, D.T. Vistica, C. Hose, J. Langley, P. Cronise, A .VaigroWoiff, M. Gray-Goodrich, H. Campbell, J. Mayo, and M.R. Boyd. 1991. Feasibility of a high-flux anticancer tumor cell lines. J. Natl' Cancer Inst. 83 : 757-766   DOI
9 Recio, M.C., R.M. Giner, S. Manez, A.Talens, L. Cubells, J. Gueho, H.R. Julien, K. Hostettmann, and J.R. Rios. 1995. Antiflammary activity of flavonol glycosides from Erythrospermum monticolum depending on single or repeated local TPA adminstration. Planta Medica 61 : 502-504   DOI   ScienceOn
10 Joseph, T., C. Josiane, and C. Pierre. 1986. Antioxidant activity of flavonoids and reactivity with peroxy radical. Phytochemistry 25 : 383-385   DOI   ScienceOn
11 Plumb, G.W., S. De Pascual Teresa, C. Santos Buelga, V. Cheynier, and G. Williamson. 1998. Antioxidant properties of catechins and proanthocyanidins: effect of polymerisation, galloylation and glycosylation. Free Radical Research 29 : 351-358   DOI   ScienceOn
12 Snook, M.E., R.C. Gueldner, N.W. Widstrom, B.R. Wiseman, D.S. Himmelsbach, J.S. Harwood, and C.E. Costello. 1993. Levels of maysin and maysin analogues in silk of maize germplasm. J. Agri. Food Chem. 41 : 1481-1485   DOI   ScienceOn
13 Zamble, D.B. and S.J. Lippard. 1995. Cisplatin and DNA repair in cancer chemistry. TIBS 20 : 435-439
14 Masafumi, O., K. Junei, N. Toshihiro, and O. Matsateru. 2001. DPPH (l, I-Diphenyl-2-picrylhydrazyl) radical scavenging activity of flavonoids obtained from some medicinal plants. Bioi. Pharm. Bull. 24 : 1202-1205   DOI   ScienceOn
15 Monks, L.V., R.H. Shoemaker, K.D. Paull, R.M. Simon, S. Tosini, P. Skehan, D.A. Scudiero, A Monks, and M.R. Boyd. 1990. Comparison of in vitro anticancer drug screening data generated with a tetrazolium assay versus a protein assay against a diverse panel of human tumor cell lines. J. Natl' Cancer Inst. 82 : 1113-1118   DOI
16 Snook, M.E., N.W. Widstrom, B.R. Wiseman, P.E. Byrne, J.S. Harwood, and C.E. Costello. 1995. New C-4-hydroxy derivatives of maysin and 3 -methoxymaysin isolated from com silks (Zea mays). J. Agri. Food Chem. 43 : 2740-2745   DOI   ScienceOn
17 Widstrom, N.W. and M.E. Snook. 1998. Genetic variation for maysin and its analogues in crosses among corn inbreds. Crop Sci. 38 : 372-375   DOI   ScienceOn
18 Richard, C.G., M.E. Snook, N.W. Widstrom, and B.R. Wiseman. 1992. TLC screen for maysin, chlorogenic acid, and other possible resistance factors to the fall armyworm and the corn earworm in Zea mays. J. Agri. Food Chem. 40 : 1211-1213   DOI
19 Senba, Y., T. Nishishita, K. Saito, H. Yoshioka, and H. Yoshioka. 1999. Stopped-flow and spectrophotometric study on radical scavenging by tea catechins and the model compounds. Chem. Pharm. Bull. 47 : 1369-1374   DOI
20 Brown, J.E., and C.A. Rice-Evans. 1998. Luteolin-rich artichoke extract protects low density lipoprotein from oxidation in vitro. Free Radical Research 29 : 247-255   DOI   ScienceOn