Browse > Article

Effect of Drought Stress on Carbohydrate Composition and Concentration in White Clover  

Kim, Tae-Hwan (Department of Animal Science & Institute of Agriculture Science and Technology, College of Agriculture, Chonnam National University)
Lee, Bok-Rye (Department of Animal Science & Institute of Agriculture Science and Technology, College of Agriculture, Chonnam National University)
Jung, Woo-Jin (Department of Animal Science & Institute of Agriculture Science and Technology, College of Agriculture, Chonnam National University)
Kim, Dae-Hyun (Department of Animal Science & Institute of Agriculture Science and Technology, College of Agriculture, Chonnam National University)
Kim, Kil-Yong (Department of Biological & Environmental Chemistry, College of Agriculture, Chonnam National University)
Publication Information
KOREAN JOURNAL OF CROP SCIENCE / v.47, no.1, 2002 , pp. 48-53 More about this Journal
Abstract
To investigate the changes in the composition and pool size of carbohydrates under drought stress, white clover (Triforium repens L.) were exposed to -0.04 Mpa(well-watered, control) or to -0.12 Mpa (drought-stressed) of soil water potential during 28 days. Dry weight of leaves in drought-stressed plants was remarkably decreased by 45% within 14 days and 74% within 28 days compared to those of the control. Glucose concentration in drought-stressed plants was increased, while that of control was slightly decreased or remained at same level throughout experimental period. Fructose and sucrose concentrations in leaves were not significantly changed for drought-stressed plants, but those of the control were significantly decreased on plant after 14 days. Fructose and sucrose concentrations in stolon of control plants were sharply decreased, while that of drought-stressed plants was less varied. Those concentrations in roots were generally increased in drought-stressed plants. The concentration of total soluble sugars at 28 day was 438.0 and 632.6 mg $g^{-l}$ dwt. in control and drought stressed plants, respectively. Starch concentration of stolon and roots of control plants was significantly increased to 2.0 and 1.4 times of initial level, respectively, whereas those of drought stressed plants was nearly same level or slightly decreased compared to initial level.l.
Keywords
Glucose; Fructose; Sucrose; Starch; Drought stress; White clover;
Citations & Related Records
연도 인용수 순위
  • Reference
1 D$\ddot{u}$ring, H. 1985. Osmotic adustment in grapevines. Acta Horticul. 171 : 315-322
2 Rodrigues, M. L., M. M. Chaves, R. Wendler, M. M. David, W. P. Quick, R. C. Leegood, M. Stitt, and J. S. Pereira. 1993. Osmotic adjustment in water stressed grapevine leaves in relation to carbon assimilation. Aust. J. Plant Physiol. 20 : 309-321   DOI
3 Gunasekera, D. and G. A. Berkowitz. 1993. Use of transgenic plants with ribu1ose-1,5-bisphosphate carboxylase/oxygenase antisence DNA to evaluate the rate limitation of photosynthesis under water stress. Plant Physiol. 103 : 629-635   DOI
4 Jupp, A. P. and E. I. Newman. 1987. Morphological and anatomical effects of severe drought on the roots of Lolium perenne L. New Phytol. 105:393-402   DOI   ScienceOn
5 Kann, S. C., J. D. Everard, and W. H. Loescher. 1993. Growth, salt tolerance and mannitol accumulation in celery. HortSci. 28 :486
6 Vassey, T. L. and T. D. Sharkey. 1989. Mild water stress of phaselous vulgaris plants leads to reduced starch synthesis and extractable sucrose phosphate synthase activity. Plant Physiol 89: 1066-1070   DOI   ScienceOn
7 Lucero, D. W., P. Grieu, and A. Guckert. 2000. Water deficit and plant competition effects on growth and water-use efficiency of white clover (Trifolium repens, L.) and ryegreass (Lolium perenne, L.). Plant and Soil. 227 : 1-15   DOI
8 Munns, R. and R. Weir. 1981. Contribution of sugars to osmoticadjustment in elongating and expanding zones of wheat leaves during moderate water deficits at two light levels. Aust. J. Plant Physiot. 8 : 93-105   DOI
9 Peterson, C. A. 1987. The exodermal caspahan band of onion roots blocks the apoplastic movement of sulphate ions. J. Exp. Bot. 38: 2068-2081   DOI
10 Wang, Z. B. Quebedeaux, and G. W. Stutte. 1995. Osmotic Adjustment: Effect of Water Stress on Carbohydrates in Leaves, Stems and Roots ofApple. Aust. J. Plant Physiol. 22 : 747-754   DOI   ScienceOn
11 Geigenberger, P., R. Reimholz, U. Deiting, U. Sonnewald, and M. Stitt. 1999. Decreased expression of sucrose phosphate synthase strongly inhibits the water stress-induced synthesis of sucrose in growing potato tubers. Plant J. 19(2): 119-129   DOI   ScienceOn
12 Volaire, R, H. Thomas, N. Bertagne, E. Bourgeoils, M-F. Gautier, and F. Lelievre. 1998. Survival and recovery of perennial forage grasses under prolonged Mediterranean drought. II. Water status, solute accumulations, abscisic acid concentration and accumulation of dehydrin transcripts in bases of immature leaves. New Phytol. 140 : 451-460   DOI   ScienceOn
13 Quick, R, G. Siegl, H. E. Neuhaus, R. Fei, and M. Stitt. 1989. Short term water stress leads to a stimulation of sucrose synthesis by activating sucrose phosphate-synthase. Planta. 177 :536-546
14 Zhang, B. and D. D. Archbold. 1993. Solute accumulation in leaves of a Fragaria chiloensis and a F. virginiana selection responds to water deficit stress. J. Ame. Soci. Horticul. Sci. 118 : 208-285
15 Volaire, F. 1995. Growth, carbohydrate reserves and drought survival strategies of contrasting Dactylis glomerata populations in a Mediterranean environment. J. Applied Ecol. 32 : 56-66   DOI   ScienceOn
16 Wang, Z. B. Quebedeaux, and G. W. Stutte. 1995. Osmotic Adjustment: Effect of Water Stress on Carbohydrates in Leaves, Stems and Roots of Apple. Aust. J. Plant Physiol. 22 : 747-754   DOI   ScienceOn
17 Nabors, M. W. 1990. Environmental stress resistance procedure and applications, in: J.D. Philip (Ed.), Plant Cell Line Selec-tion, VCH, Weinheim, pp. 167-185
18 Thomas, H. 1997. Drought resistance in plants, In: S. D. Amarjit, K. B. Ranjit (Eds.), Mechanisms of Environmental Stress Resistance in plants, Harwood. pp. 1-42
19 Moorby, J., R. Munns, and J. Walcott. 1975. Effect of water deficit on photosynthesis and tuber metabolism in potatoes. Aust. J. Plant Physiol. 2 ..323-333   DOI
20 Watt, M., C. M. van der Weele, M. E. McCully, and M. J. Canny. 1996. Effects of local variations in soil moisture on hydrophobic deposits and dye diffusion in corn roots. Bot. Acta. 109 :492-501   DOI
21 Van Handel, E. 1968. Direct microdetermination of sucrose. Anal Biochem. 22 : 1341-1346
22 Frensch, J. 1997. Primary responses of root and leaf elongation towater deficits in the atmosphere and soil solution. J. Exp. Bot. 48 : 985-999
23 Cornic, G. 1994. Drought stress and high light effects on leaf photosynthesis. In: N.R.Baker, ed. Photoinhibition of photosynthesis : From Molecular Mechanisms to the Field. Oxford, UK:BI0S. pp.297-313
24 Dancer, J., M. David, and M. Stitt. 1990. Water stress leads to a change of partitioning in favor of sucrose in heterotrophic cell suspension culture of Chenopodium rubrum. Plant Cell and Environ.13 : 957-963   DOI
25 Sacks, M. M., W. K. Silk, and P. Burman. 1997. Effect of water stress on cortical cell division rates within the apical meristem of primary roots of maize. Plant Physiol. 114 : 519-527   DOI
26 Gimenez, C., V. J. Mitchell, and D. W. Lawlor. 1992. Regulation of photosynthetic rate of two sunflower hybrids under water stress. Plant Physiol. 98 : 516-524   DOI   ScienceOn
27 Zrenner, R. and M. Stitt. 1991. Comparison of effect of rapidly and gradually developing water-stress on carbohydrate metabolism in spinach leaves. Plant Cell Environ. 14 : 939-946   DOI
28 Seiffert, S., J. Kaselowsky, A. Jungk, and N. Claassen. 1995. Observed and calculated potassium uptake by maize as affected by soil water content and bulk density. Agron. J. 87 :1070-1077   DOI
29 Premachandra, G. S., H. Saneoka, K. Fujita, and S. Ogata. 1992. Leaf water relations, osmotic adjustment, cell membrane stability, epicuticular wax load and growth as affected by increasing water deficits in sorghum. J. Exp. Bot. 43 : 1569-1576   DOI
30 Boyer, J. S. 1982. Plant productivity and environment. Sci. 218 :443-448   DOI   ScienceOn
31 Wang, Z. and G. W. Stutte. 1992. The role of carbohydrates in active osmotic adjustment in apple under water stress. J. Ame. Soci. Horticul. Sci. 117 : 816-823
32 Bloem, J., P. C. De Ruiter, G. J. Koopman, G. Lebbink, and L.Brussaard. 1992. Microbial numbers and activity in dried andrewetted arable soil under integrated and conventional management. Soil Biol. Biochem. 24 : 655-665   DOI   ScienceOn
33 Meyer, R. F. and J. S. Boyer. 1981. Osmoregulation, solute distribution and growth in soybean seedlings having low water potentials. Planta. 151 : 482-489   DOI
34 Morgan, J. M. 1984. Osmoregulation and water stress in higher plants. Annu. Rev. PIant Physiot. 35 : 299-213   DOI   ScienceOn
35 Chaves, M. M. 1991. Effects of water deficits on carbon assimilation.J. Exp.Bot.42: 1-16   DOI
36 Walwoth, J. L. 1992. Soil drying and rewetting, or freezing and thawing, affects soil solution composition. Soil Sci. Soc. Am. J. 56: 433-437   DOI
37 Davis, J. S. and J. E. Gander. 1967. A re-evaluation of the Roe procedure for the determination of fructose. Anal. Biochem. 19 :72-79   DOI   ScienceOn
38 Geigenberger, P, R. Reimholz, M. Geiger, L. Merlo, V. Canale, and M. Stitt. 1997. Regulation of sucrose and starch metabolism in potato tubers in response to short-term water deficit. Planta. 201 : 502-518   DOI   ScienceOn
39 Bray, E. A. 1997. Plant response to water deficit, Trends Plant Sci 2 : 48-54   DOI   ScienceOn
40 Bohnert, H. J., D. E. Nelson, and R. G. Jensen 1995. Adaptations to environmental stresses. Plant Cell. 7 : 1099-1111   DOI   ScienceOn