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http://dx.doi.org/10.22156/CS4SMB.2020.10.10.143

The Convergence Effect of Phloretin Existent in Plants on Vascular Contractility  

Bang, Joon Seok (College of Pharmacy, Sookmyung Women's University)
Je, Hyun Dong (Dept. of Pharmacology, College of Pharmacy, Daegu Catholic University)
Min, Young Sil (Dept. of Pharmaceutical Science, Jungwon University)
Publication Information
Journal of Convergence for Information Technology / v.10, no.10, 2020 , pp. 143-149 More about this Journal
Abstract
This study tried to observe the ability to inhibit vasocontriction in phloretin - the primary ingredient of apple tree leaves and the Manchurian apricot - through ROCK(Rho-associdated, coiled-coil containing protein kinase) inactivation in rat aortae. A piece of artery that was separated from Sprague-Dawley male rats and retained or damaged the endothelium was suspended in myograph tank with two metal rings, the lower ring fixed to the bottom of the tank, and the upper ring connected to the isotonic force transducer. Interestingly, phloretin inhibited fluoride- or phorbol ester-provoked contraction implying that additional pathways dissimilar from endothelial nitric oxide synthesis such as ROCK or MEK (mitogen activated protein kinase kinase) inactivation might be involved in the vasorelaxation. Therefore, this study provides that phloretin participates in the reduction of ROCK or MEK activity in smooth muscle in addition to the endothelial-dependent action of the endotheliuim in complete blood vessels, and consequently inhibits actin-myosin interaction in smooth muscle. Furthermore, phloretin inhibited thromboxane A2-induced contraction suggesting the mechanism including inhibition of ROCK and MEK.
Keywords
Endothelium; Convergence; Phloretin; Vasoconstriction; ROCK(Rho-associdated, coiled-coil containing protein kinase);
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1 M. Uehata et a.l (1997). Calcium sensitization of smooth muscle mediated by a Rho-associated protein kinase in hypertension. Nature, 389(6654), 990-994. DOI : 10.1038/40187   DOI
2 S. Sakurada et al. (2003). $Ca^{2+}$-dependent activation of Rho and Rho-kinase in membrane depolarization-induced and receptor stimulation-induced vascular smooth muscle contraction. Circ Res, 93(6), 548-556. DOI : 10.1161/01.RES.0000090998.08629.60   DOI
3 T. Kitazawa, M. Masuo & A.P. Somlyo. (1991). G protein-mediated inhibition of myosin light-chain phosphatase in vascular smooth muscle. Proc Natl Acad Sci USA, 88(20), 9307-9310.   DOI
4 A. Gohla, G. Schultz & S. Offermanns. (2000). Roles for G(12)/G(13) in agonist-induced vascular smooth muscle cell contraction. Circ Res, 87(3), 221-227. DOI : 10.1161/01.res.87.3.221   DOI
5 T. Leung et al. (1995), A novel serine/threonine kinase binding the Ras- related RhoA GTPase which translocates the kinase to peripheral membranes. J Biol Chem, 270(49), 29051-29054. DOI : 10.1074/jbc.270.49.29051   DOI
6 T. Matsui et al. (1996). Rho-associated kinase, a novel serine/threonine kinase, as a putative target for small GTP binding protein Rho. EMBO J, 15(9), 2208-2216   DOI
7 W. G. Wier & K. G. Morgan. (2003). Alpha1-adrenergic signaling mechanisms in contraction of resistance arteries. Rev Physiol Biochem Pharmacol, 150, 91-139. DOI : 10.1007/s10254-003-0019-8   DOI
8 S. B. Jeon et al. (2006). A role for Rho kinase in vascular contraction evoked by sodium fluoride. Biochem Biophys Res Commun, 343(1), 27-33. DOI : 10.1016/j.bbrc.2006.02.120   DOI
9 D. P. Wilson et al. (2005). Thromboxane $A_2$-induced contraction of rat caudal arterial smooth muscle involves activation of $Ca^{2+}$ entry and $Ca^{2+}$ sensitization: Rho-associated kinase-mediated phosphorylation of MYPT1 at Thr-855, but not Thr-697. Biochem J , 389(Pt3), 763-774. DOI : 10.1042/BJ20050237   DOI
10 B. Bhattacharya & R. E. Roberts. (2003). Enhancement of alpha2-adrenoceptor-mediated vasoconstriction by the thromboxane-mimetic U46619 in the porcine isolated ear artery: role of the ERK-MAP kinase signal transduction cascade. Br J Pharmacol, 139(1), 156-162. DOI : 10.1038/sj.bjp.0705208   DOI
11 W. Zheng et al (2018). The protective effect of phloretin in osteoarthritis: an in vitro and in vivo study. Food Funct, 9(1), 263-278. DOI : 10.1039/c7fo01199g   DOI
12 H. Duan et al (2017). Phloretin induces apoptosis of human esophageal cancer via a mitochondria-dependent pathway. Oncol Lett, 14(6), 6763-6768. DOI : 10.3892/ol.2017.7037
13 R. S. Garcha, P. S. Sever, & A. D. Hughes. (2001). Mechanism of action of angiotensin II in human isolated subcutaneous resistance arteries. Br J Pharmacol, 134(1), 188-196. DOI : 10.1038/sj.bjp.0704222   DOI
14 Y. S. Min et al. (2018). Endothelium Independent Effect of Pelargonidin on Vasoconstriction in Rat Aorta. Biomol Ther(Seoul), 26(4), 374-379. DOI : 10.4062/biomolther.2017.197   DOI
15 H. J. Choi & S. I. Lee. (1989). Alpiniae katsumadaii Semen, In Korean Herbal Pharmacopoeia, Korean Medical Index Co., Seoul, Korea, p. 373. (Oniline). http://www.law.go.kr/LSW//admRulInfoP.do?adm RulSeq=4329#
16 M. Grann et al. (2016). Mechanisms Involved in Thromboxane A 2 -induced Vasoconstriction of Rat Intracavernous Small Penile Arteries. Basdic Clin Pharmaciol Toxicol, 119(3), 86-95. DOI : 10.1111/bcpt.12544   DOI
17 H. H. Chung et al. (2010). The xanthine derivative KMUP-1 inhibits models of pulmonary artery hypertension via increased NO and cGMP-dependent inhibition of RhoA/Rho kinase. Br J Pharmacol, 160(4), 971-986. DOI : 10.1111/j.1476-5381.2010.00740.x   DOI
18 L. J. Appel et al. (2003). Effects of comprehensive lifestyle modification on blood pressure control: main results of the PREMIER clinical trial. JAMA, 289(16), 2083-2093. DOI : 10.1001/jama.289.16.2083   DOI
19 M. Kuroyanagi, T. Noro & S. Fukushima. (1983). Studies on the constituents of the seeds of Alpinia katsumadai Hayata. Chemical and Pharmaceutical Buletin, 31(5), 1544-1550. DOI : 10.1248/cpb.31.1544   DOI
20 Y. Yang et al. (1999). Two novel anti-emetic principles of Alpinia katsumadai. J Natl Prod, 62(12), 1672-1674. DOI : 10.1021/np990096e   DOI
21 H. Duan et al. (2017). Phloretin induces apoptosis of human esophageal cancer via a mitochondria-dependent pathway. Oncol Lett, 14(6), 6763-6768. DOI : 10.3892/ol.2017.7037
22 A. P. Somlyo & A. V. Somlyo. (1994). Signal transduction and regulation in smooth muscle. Nature, 372(6503), 231-236. DOI : 10.1038/372231a0   DOI
23 A. P. Somlyo & A. V. Somlyo. (1998). From pharmacomechanical coupling to G-proteins and myosin phosphatase. Acta Physiol Scand., 164(4), 437-448. DOI : 10.1046/j.1365-201X.1998.00454.x   DOI