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http://dx.doi.org/10.22156/CS4SMB.2019.9.8.274

Suppression of IgE and GATA-3-dependent Th2 Cell Regulation by PM-E and PM-70M Isolated Polygonum Multiflorum  

Choi, Eun-Young (RXAP KOREA)
Publication Information
Journal of Convergence for Information Technology / v.9, no.8, 2019 , pp. 274-281 More about this Journal
Abstract
This study examined the effects of GATA-3-dependent Th2 cells and IgE inhibition by using PM-E and PM-70M isolated from Sasao. It was analyzed that the 70 % methanol layer contained adsorbed chromatograms of the fraction of sodium hypochlorite that inhibited GATA-3 transcription factor activity. As a result, PM-70 %MFL fraction seems to have an antiallergic effect by inhibiting GATA-3 which regulates Th2 cytokine. PM-30A, PM-70A, and PM-30A layers by inhibiting IgE secretion in B cells by co-culturing the fractions with anti-CD40/rmIL-6 and TNF-${\alpha}$ mRNA and IgE were not different from control. However, expression of IL-6 and TNF-${\alpha}$ mRNA was $0.69{\pm}0.058$ (p<0.001) and $0.72{\pm}0.58$ (p<0.05), respectively. The amount of IgE secretion was $94.6{\pm}16.0$, which was significantly decreased by 45.6% (p<0.01) compared with the control group. This study suggests that the PM-70% MFL layer in the fraction of Sasao contains the components that inhibit the differentiation and activity of B cells.
Keywords
Polygonum multiflorum; Atopic dermatitis PM-70 %MFL; IL-6; TNF-${\alpha}$ mRNA; IgE;
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