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http://dx.doi.org/10.4313/JKEM.2007.20.1.092

Characteristics of Low-level Light Source for Animal Cell Proliferation  

Cheon, Min-Woo (조선대학교 의과대학)
Kim, Seong-Hwan (조선대학교 의과대학)
Song, Chang-Hun (조선대학교 의과대학)
Mun, Seong-Pyo (조선대학교 의과대학)
Kim, Tae-Gon (동신대학교 전기전자공학과)
Park, Yong-Pil (동신대학교 전기전자공학과)
Kim, Dae-Young ((주)바이오아테코)
Kim, Young-Su ((주)바이오아테코)
Publication Information
Journal of the Korean Institute of Electrical and Electronic Material Engineers / v.20, no.1, 2007 , pp. 92-97 More about this Journal
Abstract
This paper performed the basic study for developing the Photodynamic Therapy Equipment for medical treatment. We developed the equipment palpating cell proliferation using a high brightness LED. This equipment was fabricated using a micro-controller and a high brightness LED, and designed to enable us to control light irradiation time, intensity, frequency and so on. Especially, to control the light irradiation frequency, FPGA was used, and to control the change of output value, TLC5941 was used. Control stage is divided into 30 levels by program. Consequently, the current value could be controlled by the change of level in Continue Wave(CW) and Pulse Width Modulation(PWM), and the output of a high brightness LED could be controlled stage by stage. And then, each experiment was performed to irradiation group and non-irradiation group for both Rat bone marrow and Rat tissue cells. MTT assay method was chosen to verify the cell increase of two groups and the effect of irradiation on cell proliferation was examined by measuring 590 nm transmittance of ELISA reader. As a result, the cell increase of Rat bone marrow and tissue cells was verified in irradiation group as compared to non-irradiation group. The fact that specific wavelength irradiation has an effect on cell vitality and proliferation is known through this study.
Keywords
Low-level light source; Optical power; Animal cell; MTT assay;
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