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http://dx.doi.org/10.9729/AM.2013.43.1.1

Improvement of Alignment Accuracy in Electron Tomography  

Jou, Hyeong-Tae (Maritime Security Center, Korea Institute of Ocean Science & Technology)
Lee, Sujeong (Mineral Resources Research Division, Korea Institute of Geoscience and Mineral Resources)
Kim, Han-Joon (Maritime Security Center, Korea Institute of Ocean Science & Technology)
Publication Information
Applied Microscopy / v.43, no.1, 2013 , pp. 1-8 More about this Journal
Abstract
We developed an improved method for tilt series alignment with fiducial markers in electron tomography. Based on previous works regarding alignment, we adapted the Levenberg-Marquardt method to solve the nonlinear least squares problem by incorporating a new formula for the alignment model. We also suggested a new method to estimate the initial value for inversion with higher accuracy. The proposed approach was applied to geopolymers. A better alignment of the tilt series was achieved than that by IMOD S/W. The initial value estimation provided both stability and a good rate of convergence since the new method uses all marker positions, including those partly covering the tilt images.
Keywords
Electron tomography; Geopolymer; Alignment; Fiducial marker;
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Times Cited By KSCI : 1  (Citation Analysis)
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