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http://dx.doi.org/10.9729/AM.2012.42.1.009

Quantitative Analysis of Neurotransmitters in the Endings Presynaptic to Vibrissa Afferent Terminals in the Cat Trigeminal Caudal Nucleus  

Kim, Yun-Sook (Department of Oralanatomy and Neurobiology, Kyungpook National University)
Mun, Cheol-Ju (Department of Oralanatomy and Neurobiology, Kyungpook National University)
Cho, Jin-Hyun (Department of Prosthetics, School of Dentistry, Kyungpook National University)
Bae, Jin-Young (Department of Oralanatomy and Neurobiology, Kyungpook National University)
Na, Yeon-Kyung (Division of Basic Nursing Science, College of Nursing, Kyungpook National University)
Bok, Hye-Jeong (Department of Dental Hyegine, Busan Women's College)
Bae, Yong-Chul (Department of Oralanatomy and Neurobiology, Kyungpook National University)
Paik, Sang-Kyoo (Department of Oralanatomy and Neurobiology, Kyungpook National University)
Publication Information
Applied Microscopy / v.42, no.1, 2012 , pp. 9-16 More about this Journal
Abstract
The goal of this study was to identify neurotransmitters in endings (p-endings) presynaptic to low-threshold mechanoreceptive vibrissa afferents in the laminae III/IV of cat trigeminal caudal nucleus (Vc). Rapidly-adapting vibrissa afferents were intra-axonally labeled after electrophysiological identification, and postembedding immunogold staining with antisera against ${\gamma}$-aminobutyric acid (GABA) and glycine was performed, followed by quantitative ultrastructural analysis of p-endings presynaptic to the labeled vibrissa afferent terminals. Sixteen p-endings, which are presynaptic to the HRP-labeled vibrissa afferent terminals, were analyzed in this study: Eight p-endings (50%, 8/16) were immunopositive to GABA but immunonegative to glycine (GABA+ p-ending), and remaining 8 p-endings (50%, 8/16) exhibited immunoreactivity to both GABA and glycine. Bouton volume of the p-endings was not significantly different between the two groups. However, the p-endings differed from each other in relative content of GABA and glycine. These findings suggest that low-threshold mechanoreceptive information conveyed through vibrissa afferent at Vc is presynaptically modulated by GABA and/or glycine, and that degree of presynaptic modulation may differ among each vibrissa afferent terminal.
Keywords
Presynaptic endings; Vibrissa afferent; Immunogold; GABA; Glycine;
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