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The Effect of LhGH on Hair Regeneration in C57BL/6CrN Mouse  

Kim, Yong-Ju (Department of Life Science, College of Natural Science, Hallym University)
Kim, Tae-Keun (Department of Life Science, College of Natural Science, Hallym University)
Min, Byoung-Hoon (Department of Parasitology, Korea University College of Medicine and Institute of Travel Medicine Korea University)
Kim, Soo-Jin (Department of Life Science, College of Natural Science, Hallym University)
Publication Information
Applied Microscopy / v.41, no.1, 2011 , pp. 47-53 More about this Journal
Abstract
Hair is an appendage of skin which protects the body from outer physical and chemical stimuli. Hair is generated from the hair follicle lying on a sunken basal layer of epidermis. Hair cycling, which regenerates hair follicles throughout the life time of the organism. Numerous kinds of factors which exist at the hair follicle have been reported to regulate hair cycling, Human growth hormone secreted from pituitary gland, initially demonstrated to accelerate organ's growth, has been reported to play a role in the biology of organ size determination. We investigated the effect of 6-histidines residues tagged at amino-terminus of human growth hormone using light and electronmicroscopic methods. Human growth hormone encapsulated in nano-liposome (LhGH) was used to find how LhGH affects hair follicle cycling of mouse (C57BL6/CrN). Distilled water as a negative control, 3% Minoxidil as a positive control, and LhGH were applied to mouse for weeks. LhGH increased the number of exposed hairs per given areas ($1mm^2$). This result was also confirmed using a different breed of mice which show natural hair loss in an old age (about 17 months after birth). When LhGH was applied for 3 weeks after natural hair loss, natural hair loss on these mice was prevented, However, the control group mice on which LhGH was not applied showed further hair loss. This result indicates that LhGH may stimulate hair cycling of mouse. In clusion, it is cleat that the LhGH increased the number of hair on mice and help the depilated skin to grow new hair follicles again.
Keywords
hair; liposome; LhGH; human growth hormone; C57BL6/CrN mouse;
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Times Cited By KSCI : 1  (Citation Analysis)
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1 Seven MR, Bori H, Carina VDV, Stefan E, Kerstin F, Ian AM, Kurt SS, Ralf P: A comprehensive guide for the accurate classification of murine hair follicles in distinct hair cycle stages. J Invest Dermatol 117(1) : 3-15, 2001.   DOI   ScienceOn
2 Tsuboi R: Growth factors and hair growth. Korean Journal of Investigative Dermatology 4(2) : 103-108, 1997.
3 Yarosh DB: Liposomes in investigative dermatology. Photodermatol Photoimmunol Photomed 17(5) : 203-12, 2001.   DOI   ScienceOn
4 Kim YI, Lee, MW, Kwon J, Song CH, Lee CH: Experimental studies on the factors related to hair loss in spontaneous hair loss C57BL/6N mice models. The Korean J Anat 38(2) : 133-143, 2005.
5 Kuag HS: Science of hair, pp. 36-140, Sumoonsa, Seoul, 2001. (Korean)
6 Li LF, Guo J, Gao ZF: Overexpression of skin protein kinase Calpha in anagen hair follicles during induced growth of mouse hair. Clin Exp Dermatol 28(4) : 429-433, 2003.   DOI   ScienceOn
7 Messenger AG: The control of hair growth and pigmentation, In : Elise A Olsen, Disorders of Hair Growth, McGraw-Hill, pp. 39- 58, New York, 1994.
8 Messenger AG, Rundegren J: Minoxidil: mechanisms of action on hair growth. Br J Dermatol 150(2) : 186-194, 2004.   DOI   ScienceOn
9 Muller HK, Rook AJ, Kubba R: Immunohistology and autoantibody studies in alopecia areata. Br J Dermatol 102: 609-610, 1980.   DOI   ScienceOn
10 Mou C, Jackson B, Schneider P, Overbeek PA, Headon DJ: Generation of the primary hair follicle pattern. Proc Natl Acad Sci 103(24) : 9075-9080, 2006.   DOI   ScienceOn
11 Nystrom HC, Klintland N, Caidahl K, Bergstrom G, Wickman A: Short-term administration of growth hormone (GH) lowers blood pressure by activating eNOS/nitric oxide (NO)-pathway in male hypophysectomized (Hx) rats. BMC Physiol 5 : 4-17, 2005.   DOI
12 Selmanowilz VJ, Victor S, Warburton D, Orentreich N: Fingerprints arches in alopecia areata. Arch Dermatol 110(4) : 570-571, 1974.   DOI   ScienceOn
13 Orland GF: Structure of the skin In: Goldsmith LA, ed, Biochemistry and the Physiology of the Skin 1, pp. 3-63, Oxford University Press, Oxford, 1983.
14 Piao YJ, Kim YD, Seo EY, Song KY, Suhr KB, Lee JH, Park JK: Expression of the proteins associated with epidermal differentiation during human hair growth cycle. Korean Journal of Investigative Dermatology 9(1) : 21-28, 2002.
15 Roberts MS, Walters KA: The relationship between structure and barrier function in skin. In: Roberts MS, Walters KA, eds, Dermal Absorption and Toxicity Assessment, pp. 1-42, Marcel Dekker Inc, New York, 2008.
16 Friedman PS: Alopecia areata and auto-immunity. Br J Dermatol 105 : 153-157, 1981.   DOI   ScienceOn
17 Hansen LA, Alexander N, Hogan ME, Sundberg JP, Dlugosz A, Threadgill DW, Magnuson T, Yuspa SH: Genetically null mice reveal a central role for epidermal growth factor receptor in the differentiation of the hair follicle and normal hair development. Am J Pathol 150(6) : 1959-1975, 1997.
18 Herndon DN, Hawkins HK, Nguyen TT, Pierre E, Cox R, Barrow RE: Characterization of growth hormone enhanced donor site healing in patients with large cutaneous burns. Ann Surg 221(6) : 649-659, 1995.   DOI   ScienceOn
19 Jankovic SM, Jankovic SV: The control of hair growth. Dermatol Online J 4(1) : 2, 1998.
20 Alonso L, Okada H, Pasolli HA, Wakeham A, You-Ten AI, Mak TW, Fuchs E: Sgk3 links growth factor signaling to maintenance of progenitor cells in the hair follicle. J Cell Biol 170(4) : 559- 570, 2005.   DOI   ScienceOn
21 Beak SJ, Lee GY, Oh DK: Compounds for anti hairloss or promote of hair growth to contained Human Growth Hormone. An applicant for a patent Regeron Inc., 2005. (Korean)
22 Bergfeld WF: Diffuse hair loss in women. Cutis 22(1) : 190-195, 1978.
23 Botchkareva NV, Khlgatian M, Longley BJ, Botchkarev V, Gilchrest BA: SCF/c-kit signaling is required for cyclingregeneration of the hair pigmentation unit. FASEB 15 : 645-658, 2001.   DOI
24 Elias PM: Epidermal lipids, membranes and keratinization. Int J Dermatol 20(1) : 1-19, 1981.   DOI
25 Elias PM: Epidermal lipids, barrier function and desquamation. J. Invest Dermatol 80(Suppl 1) : 44s-49s, 1983.
26 Fenske NA., Lober CW: Structural and functional changes of normal aging skin. J. Am Acad Dermatol 15(4 Pt 1) : 571-585, 1986.   DOI