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Synergistic Effects of 5-Fluorouracil (FU) and Curcumin on Human Cervical Cancer Cells  

Ahn, Seong-Ho (Department of Genetic Engineering, Cheongju University)
Kim, Dong-Heui (Department of Environmental Medical Biology, Wonju College of Medicine, Yonsei University)
Kang, Jung-Hoon (Department of Genetic Engineering, Cheongju University)
Lee, Myeong-Seon (Department of Genetic Engineering, Cheongju University)
Publication Information
Applied Microscopy / v.40, no.4, 2010 , pp. 229-235 More about this Journal
Abstract
Cervical cancer is associated with low antioxidant status. It has a high prevalence especially amongst woman in Asia and is a leading cause of cancer death. Cancer chemotherapy in vivo improved in cases with high p53 expression in the tumor tissue. The restoration of p53 levels could be a potential strategy to increase chemoresponsiveness. Under circumstances where damage is extensive, p53 plays a direct role in trigering apoptosis. To investigate the effect of curcumin (CMN) as an antioxidant agent on anticancer agent 5-fluorouracil (5FU) induced apoptosis and p53 expression, HPV-18 positive HeLa cells were treated with noncytotoxic amounts of antioxidant. Curcumin induced apoptosis in cervical cancer cells. Morphological hallmarks of apoptosis such as nuclear fragmentation and internucleosomal fragmentation of DNA were observed. CMN caused upregulation of p53 expression, evident from Western blotting data and also increased the susceptibility/apoptosis induced by 5FU. These results show that increasing drug sensitivity of cervical cancer cells by upregulation of p53 using CMN is novel approach and could have a possible therapeutic potential in cervical cancer.
Keywords
Cervical cancer; 5-Fluorouracil; Curcumin;
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