Browse > Article

Microstructure Analysis of Rabbit and Chicken Femurs by Light Microscopy and Transmission Electron Microscopy  

Kim, Chang-Yeon (Graduate School of Analytical Science and Technology, Chungnam National University)
Kim, Eun-Kyung (Graduate School of Analytical Science and Technology, Chungnam National University)
Jeon, Tae-Hoon (Graduate School of Analytical Science and Technology, Chungnam National University)
Nam, Seung-Won (Department of Bioscience and Biotechnology, Chungnam National University)
Kim, Youn-Joong (Graduate School of Analytical Science and Technology, Chungnam National University)
Publication Information
Applied Microscopy / v.40, no.3, 2010 , pp. 155-162 More about this Journal
Abstract
Bone is a hierarchically structured composite material which has been well studied by the materials engineering community because of its unique structure and mechanical properties. Bone is a laminated organic-inorganic composite composed of primarily hydroxyapatite, collagen and water. The main mineral that gives bone's hardness is calcium phosphate, which is also known as hydroxyapatite. Light microscopy (LM) and transmission electron microscopy (TEM) were used to study the structure of femurs from chicken and rabbit. The elemental analysis was used to search variation in the distribution of calcium, potassium and oxygen in the femur. Current investigation focused on two structural scales: micro scale (arrangement of compact bone) and nano scale (collagen fibril and apatite crystals). At micro scale, distinct difference was found in microstructures of chicken femur and rabbit femur. At nano scale, we analyzed the shape and size of apatite crystals and the arrangement of collagen fibril. Consequently, femurs of chicken and rabbit had very similar chemical property and structures at nano scale despite of their different species.
Keywords
Chicken femur; Rabbit femur; Microstructure; Electron diffraction pattern; Hydroxyapatite;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Liu ZQ, Austin T, Thomas CDL, Clement G: Bone feature analysis using image processing techniques. Computers in Biology and Medicine 26 : 65-76, 1996.   DOI   ScienceOn
2 Carlisle EM: Silicon: a possible factor in bone calcification. Science 167 : 279, 1970.   DOI   ScienceOn
3 Rho JY, Kuhn-spearing L, Zioupos P: Mechnical properties and the hierarchical structure of bone. Medical Engineering & Physics 20 : 92-102, 1998.   DOI   ScienceOn
4 Robinson RA: An electron microscopy study of the crystalline inorganic components of bone and its relationship to the organic matrix. Journal of Bone and Joint Surgery 34A : 389-434, 1952.
5 Rubin MA, Rubine J, Jasiuk I: SEM and TEM study of the hierarchical structure of C57BL/6J and C3H/HeJ mice trabecular bone. Bone 35 : 11-20, 2004.   DOI   ScienceOn
6 Olszta MJ, Cheng X, Jee SS, Kumar R, Kim YY, Kaufman MJ, Douglas EP, Gower LB: Bone structure and formation: A new perspective. Material Science & Engineering R58 : 77-166, 2007.
7 Glimcher MJ: Bone: Nature of the calcium phosphate crystals and cellular, structural, and physical chemical mechanisms in their formation. In: Nita S, ed, Review in Mineralogy & Geochemistry 64 : 223-282, 2006.   DOI   ScienceOn
8 Gupta HS, Zioupos P: Fracture of bone tissue: The 'hows' and 'whys'. Medical Engineering & Physics 30 : 1209-1226, 2008.   DOI   ScienceOn
9 Landis WJ, Song MJ, Leith A, McEwen L, McEwen BF: Mineral and organic matrix interaction in normally calcifying tendon visualized in three dimensions by high-voltage electron microscopic tomography and graphic image reconstruction. Journal of Structural Biology 110 : 39-54, 1993.   DOI   ScienceOn
10 Rensberger JM, Watabe M: Fine structure of bone in dinosaurs, birds and mammals. Nature 406 : 619-622, 2000.   DOI   ScienceOn