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Analysis of Chemical Composition, Microstructure and Hydroxyapatite Structure for Mouse Teeth  

Kim, Eun-Kyung (Graduate School of Analytical Science and Technology, Chungnam National University)
Jeon, Tae-Hoon (Graduate School of Analytical Science and Technology, Chungnam National University)
Kim, Chang-Yeon (Graduate School of Analytical Science and Technology, Chungnam National University)
Nam, Seung-Won (Department of Bioscience and Biotechnology, Chungnam National University)
Song, Kyung (Division of Electron Microscopic Research, Korea Basic Science Institute)
Lee, Sang-Gil (Division of Electron Microscopic Research, Korea Basic Science Institute)
Kim, Youn-Joong (Graduate School of Analytical Science and Technology, Chungnam National University)
Publication Information
Applied Microscopy / v.40, no.3, 2010 , pp. 147-154 More about this Journal
Abstract
The aim of this study is to determine microstructure, chemical composition and crystal structure of hydroxyapatite for mouth teeth using optical microscopy and electron microscopy as well as electron probe micro-analysis (EPMA). Enamel, a protective cover to the teeth, consisted of rods oriented in regular and had relatively higher crystallinity and Ca component. In contrast, dentin showed a sponge-like microstructure with circular holes which were passages of dentinal tubules, and had higher Mg component than the enamel region due to its higher organic content. Hydroxyapatite crystals appeared as large rods in enamel, but as small needles in dentin. Their electron diffraction patterns were different by their crystallinity as well as by the organic content of the matrix.
Keywords
Chemical composition; Dentin; Electron diffraction pattern; Enamel; Hydroxyapatite;
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1 Tjaderhane L, Hietala EL, Larmas M: Mineral element analysis of carious and sound rat dentin by electron probe microanalyzer combined with back-scattered electron image. Journal Dental Research 74 : 1770-1774, 1995.   DOI   ScienceOn
2 White SN, Luo W, Paine ML, Fong H, Sarikaya M, Snead ML: Biological organization of hydroxyapatite crystallites into a fibrous continuum toughens and controls anisotropy in human enamel. Journal Dental Research 80 : 321-326, 2001.   DOI   ScienceOn
3 Zou XD, Sukharev Y, Hovmöller S: ELD-a computer program system for extracting intensities from electron diffraction patterns. Ultramicroscopy 49 : 147-158, 1993.   DOI
4 Nalbandian J, Gonzales F, Sognnaes RF: Sclerotic age change in root dentin of human teeth as observed by optical, electron, and X-ray microscopy. Journal of Dental Research 39 : 598, 1960.   DOI   ScienceOn
5 Oleynikov, P. eMap, a program for generating 3D Fourier maps and determining peak positions. AnaliTEX www.analitex.com/ eMap.html; Oleynikov, P. http://www.analitex.com.
6 Palmer LC, Newcomb CJ, Kaltz SR, Spoerke ED, Stupp SI: Biomimetic systems for hydroxyapatite mineralization inspired by bone and enamel. Chemical Review 108 : 4754-4783, 2008.   DOI   ScienceOn
7 Nalla RK, Porter AE, Daraio C, Minor AM, Radmilovic V, Stach EA, Tomsia AP, Ritchie RO: Ultrastructural examination of dentin using focused ion-beam cross-sectioning and transmission electron microscopy. Micron 36 : 672-680, 2005.   DOI   ScienceOn
8 Nishizawa Y, Morii H, Durlach J: New perspectives in magnesium research. Springer, Japan, pp. 359, 2006.
9 Olszta MJ, Cheng X, Jee SS, Kumar R, Kim YY, Kaufman MJ, Douglas EP, Gower LB: Bone structure and formation: A new perspective. Materials Science and Engineering R58 : 77-116, 2007.
10 Porter AE, Nalla RK, Minor A, Jinschek JR, Kisielowski C, Radmilovic V, Kinney JH, Tomsia AP, Ritchie RO: A transmission electron microscopy study of mineralization in age-induced transparent dentin. Biomaterials 26 : 7650-7660, 2005.   DOI   ScienceOn
11 Suk DH, Kim CY: Determination of age in humans from root dentin transparency. Korean Journal of Oral Medicine 7 : 59-65, 1982. (Korean)
12 Tamerler C, Sarikaya M: Molecular biomimetics: genetic synthesis, assembly and formation of materials using peptides. MRS Bulletin 5 : 504-512, 2008.
13 Glauert AM: Practical methods in electron microscopy. American Elsevier, New York, pp. 1-198, 1974.
14 Boyde A, Lester KS: An electron microscope study of fractures dentinal surfaces. Calcified Tissue Research 1 : 122-136, 1967.   DOI   ScienceOn
15 Elliott JC: Calcium phosphate biominerals. In: Matthew JK, ed, Reviews in Mineralogy and Geochemistry. Phosphates, pp. 427- 453, Mineralogical Society of America, Chantilly, 2002.
16 Fong S, White SN, Paine ML, Luo W, Snead ML, Sarikaya M: Enamel structure properties controlled by engineered proteins in transgenic mice. Journal of Bone and Mineral Research 18 : 2052-2059, 2003.   DOI   ScienceOn
17 Glimcher MJ: Bone: Nature of the calcium phosphate crystals and cellular, structural and physical chemical mechanism in their formation. In: Nita S, ed, Reviews in Mineralogy and Geochemistry. Medical Mineralogy and Geochemistry, pp. 223-282, Mineralogical Society of America, Chantilly, 2006.
18 Habelitz S, Zartoshtimanesh S, Balooch M, Marshall SJ, Marshall GW, Denbesten PK: Structure and properties of murine and human dentin. Materials Research Society 874 : L5.18.1, 2005.
19 Kinney JH, Nalla RK, Pople JA, Breunug TM, Ritchie RO: Agerelated transparent root dentin: mineral concentration, crystallite size and mechanical properties. Biomaterials 26 : 3363-3376, 2005.   DOI   ScienceOn
20 Leventouri T, Antonakos A, Kyriacou A, Venturelli R, Liarokapis E, Perdikatsis V: Crystal structure studies of human dental apatite as a function of age. International Journal of Biomaterials 698547, 2009.
21 Mann S: Molecular recognition in biomineralization. Nature 332 : 119-124, 1988.   DOI