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Microscopic Study of Decomposition-Inhibition Effect in Grapefruit-seed Extract in Rat Kidney  

Hwang, Kyu-Sung (Department of Funeral Science, College of Health Industry, Eulji University)
Choi, Ki-Ju (Electron Microscopy Laboratory, Eulji University)
Nam, Yong-Seok (Department of Anatomy Catholic Institute for Applied Anatomy, College of Medicine, Catholic University)
Lim, Do-Seon (Department of Dental Hygiene, College of Health Science, Eulji University)
Publication Information
Applied Microscopy / v.39, no.4, 2009 , pp. 325-332 More about this Journal
Abstract
It has been well known that grapefruit seed extract (GSE) have effects on the deodorization and a wide range disinfectant. But anybody do not research about the decomposition-inhibition effect of GSE. The present study was performed to investigate the decomposition-inhibition effect of GSE by measuring the microscope and electroscope observation with passage of time. A total of 30 healthy Sprague-Dawley (SD) rats weighting from 230 gm to 250 gm were used as experimental animals. One group that do not treated by GSE named control group and the other group that treated by 55% GSE named Experimental group. Under ether anesthesia, right kidney was obtained. Put each sample in $37^{\circ}C$ and humidity $80{\pm}5%$ incubator, we obtained each sample after 0 day, 1 day, 2 days, 3 days, 4 days and 5 days. 4 ${\mu}m$ of paraffin sections were obtained, stained H-E stain and observed by use of a microscope. Morphological change in tissue was similar to control 1 day group and experimental 3 days group. Therefore, decomposition-inhibition effect of GSE continued about 2 days and it protected necrosis. According to above results, the author suggest that 55% GSE is an effective decomposition inhibitor until 2 days on $37^{\circ}C$ and humidity $80{\pm}5%$ conditions.
Keywords
Decomposition prevention; Decomposition-inhibition effect; GSE (Grapefruit Seed Extract); Morphological change; Necrosis;
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