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Three Dimensional Reconstruction of Cellular Structure in Drosophila Retina Using High Voltage Electron Microscopy  

Mun, Ji-Young (School of Life Sciences and Biotechnology, Korea University)
Lee, Kyung-Eun (School of Life Sciences and Biotechnology, Korea University)
Han, Sung-Sik (School of Life Sciences and Biotechnology, Korea University)
Publication Information
Applied Microscopy / v.39, no.2, 2009 , pp. 185-189 More about this Journal
Abstract
Studies about the structure of Drosophila melanogaster retinal cell using electron microscopy have been carried out in details since 1960s. However, these results can have limitations in functional research because of two-dimensional structure. In this study, the adult retina of Drosophila melanogaster was investigated by employing high pressure freezing method, serial sections, high voltage electron microscopy, and 3-dimensional reconstruction method. From there results, mitochondria, microtubules, and nuclei were reconstructed as 3-dimensional structure using IMOD program. The 3D structure of these organelles showed that mitochondira mainly located in distal region near lens, and microtubule mainly located in distal and basal region. The 3D reconstruction of these organelles can be used for a critical evaluation in the dynamic change of cellular organelles caused by functional abnormality like retinal degeneration.
Keywords
HVEM; 3D structure; Retina; Drosophila melanogaster;
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1 Eckmiller MS: Defective cone photoreceptor cytoskeleton, alignment, feedback, and energetics can lead to energy depletion in macular degeneration. Prog Retin Eye Res 23(5) : 495-522, 2004   DOI   ScienceOn
2 Farquhar MG, Palade GE: Junctional complexes in various epithelia. J Cell Biol 17 : 375-412, 1963   DOI   ScienceOn
3 Hama K, Arii T, Kosaka T: Three-dimensional organization of neuronal and glial processes: high voltage electron microscopy. Microsc Res Tech 29(5) : 357-367, 1994   DOI   ScienceOn
4 Knabe W, Kuhn HJ: The role of microtubules and microtubuleorganising centres during the migration of mitochondria. J Anat 189(Pt 2) : 383-391, 1996
5 Perkins GA, Ellisman MH, Fox DA: The structure-function correlates of mammalian rod and cone photoreceptor mitochondria: observations and unanswered questions. Mitochondrion 4(5-6) : 695-703, 2004   DOI   ScienceOn
6 Perry MM: Further studies on the development of the eye of Drosophila melanogaster. I. The ommatidia. J Morphol 124 (2) : 227-248, 1968   DOI
7 Sandberg K: Methods for image segmentation in cellular tomography. Methods Cell Biol 79 : 769-798, 2007   DOI
8 Studer D, Graber W, Al-Amoudi A, Eggli P: A new approach for cryofixation by high-pressure freezing. J Microsc 203 (Pt 3) : 285-294, 2001   DOI   ScienceOn
9 Perkins GA, Ellisman MH, Fox DA: Three-dimensional analysis of mouse rod and cone mitochondrial cristae architecture: bioenergetic and functional implications. Mol Vis 9 : 60-73, 2003   ScienceOn
10 Muller-Reichert T, Hohenberg H, O'Toole ET, McDonald K: Cryoimmobilization and three-dimensional visualization of C. elegans ultrastructure. J Microsc 212(Pt 1) : 71-80, 2003   DOI   ScienceOn
11 Hess MW, Pfaller K, Hampolz B, Longato S, Teis D, Florl A, Gutleben K, Huber LA: Microscopy of the Drosophila facet eye: vademecum for standardized fixation, embedding, and sectioning. Microsc Res Tech 69(2) : 93-98, 2006   DOI   ScienceOn
12 Bravo-Nuevo A, Williams N, Geller S, Stone J: Mitochondrial deletions in normal and degenerating rat retina. Adv Exp Med Biol 533 : 241-248, 2003   ScienceOn
13 Monaghan P, Perusinghe N, Muller M: High-pressure freezing for immunocytochemistry. J Microsc 192(Pt 3) : 248-258, 1998   DOI   ScienceOn
14 Perkins GA, Frey TG: Recent structural insight into mitochon-dria gained by microscopy. Micron 31(1) : 97-111, 2000   DOI   ScienceOn
15 Gorska-Andrzejak J, Stowers RS, Borycz J, Kostyleva R, Schwarz TL, Meinertzhagen IA: Mitochondria are redistributed in Drosophila photoreceptors lacking milton, a kinesinassociated protein. J Comp Neurol 463(4) : 372-388, 2003   DOI   ScienceOn
16 Grayson C, Bartolini F, Chapple JP, Willison KR, Bhamidipati A, Lewis SA, Luthert PJ, Hardcastle AJ, Cowan NJ, Cheetham ME: Localization in the human retina of the X-linked retinitis pigmentosa protein RP2, its homologue cofactor C and the RP2 interacting protein Arl3. Hum Mol Genet 11(24) : 3065-3074, 2002   DOI   ScienceOn