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Cellular Imaging of Gold Nanoparticles Using a Compact Soft X-Ray Microscope  

Kwon, Young-Man (Department of Radiology and Institute for Radiological Imaging Science, Wonkwang University, School of Medcine)
Kim, Han-Kyong (Department of Radiology and Institute for Radiological Imaging Science, Wonkwang University, School of Medcine)
Kim, Kyong-Woo (Department of Radiology and Institute for Radiological Imaging Science, Wonkwang University, School of Medcine)
Kim, Sun-Hee (Department of Radiology and Institute for Radiological Imaging Science, Wonkwang University, School of Medcine)
Yin, Hong-Hua (Department of Radiology and Institute for Radiological Imaging Science, Wonkwang University, School of Medcine)
Chon, Kwon-Su (Department of Radiology and Institute for Radiological Imaging Science, Wonkwang University, School of Medcine)
Kang, Sung-Hoon (Department of Radiology and Institute for Radiological Imaging Science, Wonkwang University, School of Medcine)
Park, Seong-Hoon (Department of Radiology and Institute for Radiological Imaging Science, Wonkwang University, School of Medcine)
Juhng, Seon-Kwan (Department of Radiology and Institute for Radiological Imaging Science, Wonkwang University, School of Medcine)
Yoon, Kwon-Ha (Department of Radiology and Institute for Radiological Imaging Science, Wonkwang University, School of Medcine)
Publication Information
Applied Microscopy / v.38, no.3, 2008 , pp. 235-243 More about this Journal
Abstract
A compact soft x-ray microscope operated in the 'water window' wavelength region ($2.3{\sim}4.4nm$) was used for observing cells with nano-scale spatial resolution. To obtain cellular imaging captured with colloidal gold nanoparticles using a compact soft x-ray microscope. The colloidal gold nanoparticles showed higher contrast and lower transmission more than 7 times than that of cellular protein on the soft x-ray wavelength region. The structure and thickness of the cell membrane of the Coscinodiscus oculoides (diatome) and red blood cells were seen clearly. The gold nanoparticles within the HT1080 and MDA-MB 231 cells were seen clearly on the soft x-ray microscopy. The gold nanoparticles were aggregated within vesicles by endocytosis.
Keywords
Cellular imaging; Gold nanoparticles; Soft x-ray; X-ray microscopy;
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1 Attwood DT: Soft x-rays and extreme ultraviolet radiation. Cambridge University Press, Cambridge, p. 2, 1999
2 Chao W, Harteneck BD, Liddle JA, Anderson EH, Attwood DT: Soft x-ray microscopy at a spatial resolution better than 15 nm. Nature 435 : 1210-1213, 2005   DOI   ScienceOn
3 Faulk WP, Taylor GM: An immunocolloid method for the electron microscope. Immunochem 8 : 1081-1083, 1971   DOI   ScienceOn
4 Hoshino M, Aoki S: Laser plasma soft x-ray microscope with Wolter mirrors for observation of biological specimens in air. Jpn J Appl Phys 45 : 989-994, 2006   DOI
5 Larabell CA, Le Gros MA: X-ray tomography generates 3-D reconstructions of the yeast, Saccharomyces cerevisiae, at 60-nm resolution. Mol Biol Cell 15 : 957-962, 2004   DOI   ScienceOn
6 Son JS, Cho M, Kim DH: Prepulse effect on laser-induced water-window radiation from a liquid nitrogen jet. Appl Phys Lett 90 : 261520, 2007
7 Stollberg H, Guttmann P, Takman PAC, Hertz HM: Size-selective colloidal-gold localization in transmission x-ray microscopy. J Microscopy 225 : 80-87, 2007   DOI   ScienceOn
8 Wilhein T, Burkhard K, Fabrizio ED, Romanato F: Differential interference contrast x-ray microscopy with submicron resolution. Appl Phys Lett 78 : 2082-2084, 2001   DOI   ScienceOn
9 Wei$\beta$ D, Schneider G, Vogt S, Guttmann P, Niemann B, Rudolph D, Schmahl G: Tomographic imaging of biological specimens with the cryo transmission x-ray microscope. Nuclear Instruments and Methods in Physics Research A 467-468 : 1301-1311, 2001   DOI   ScienceOn
10 Berglund M, Rymell L, Peuker M, Wilhein T, Hertz HM: Compact water-window transmission x-ray microscopy. J Micros 197 : 268-273, 2000   DOI   ScienceOn
11 Fabrizio ED, Cojoc D, Cabrini S, Kaulich B, Susini J, Facci P, Wilhein T: Diffractive optical elements for differential interference contrast x-ray microscopy. Opt Exp 11 : 2278-2288, 2003   DOI
12 Kim KW, Kwon YM, Nam KY, Lim JH, Kim KG, Chon KS, Kim BH, Kim DE, Kim JG, Ahn BN, Shin HJ, Seungyu Rah SY, Kim KH, Chae JS, Gweon DG, Kang DW, Kang SH, Min JY, Choi KS, Yoon SE, Kim EA, Namba Y, Yoon KH: Compact soft x-ray transmission microscopy with sub-50 nm spatial resolution. Phys Med Biol 51 : 99-107, 2006   DOI   ScienceOn
13 Scherfeld D, Schneider G, Guttmann P, Osborn M: Visualization of cytoskeletal elements in the transmission x-ray microscope. J Stru Biol 123 : 72-82, 1998   DOI   ScienceOn
14 Meyer-Ilse W, Hamamoto D, Nair A, Lelievre SA, Denbeaux G, Johnson L, Pearson AL, Yager D, Legros MA, Larabell CA: High resolution protein localization using soft x-ray microscopy. J Micros l201 : 395-403, 2001
15 Nakayama S, Haramura K, Zeng GM, Daido H, Nakatsuka M, Nakai S, Katakura N, Nagata H, Aritome H: Zone plate x-ray microscope using a laser plasma source. Jpn J Appl Phys. 33 : 1280-1282, 1994   DOI   ScienceOn
16 Abraham-Peskir J, Chantler E, McCann C, Medenwaldt R, Ernst E: Ultrastructure of human sperm using x-ray microscopy. Med Sci Res 26 : 663-667, 1998
17 Cai QY, Kim SH, Choi KS, Kim SY, Byun SJ, Kim KW, Park SH, Juhng SK, Yoon KH: Colloidal gold nanoparticles as a bloodpool contrast agent for x-ray computed tomography in mice. Invest Radiol 42 : 797-806, 2007   DOI   ScienceOn
18 Vogt U, Lindblom M, Jansson PAC, Tuohimaa TT, Holmberg A, Hertz HM: Single-otical-element soft-x-ray interferometry with a laser-plasma x-ray source. Opt Lett 30 : 2167-2169, 2005   DOI   ScienceOn
19 http://www-cxro.lbl.gov
20 Takman PAC, Stollberg H, Johansson GA, Holmberg A, Lindblom M, Hertz HM: High-resolution compact x-ray microscopy. J Micros 223 : 175-181, 2007