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Techniques for Cryo-electron Tomography in Biological Field  

Mun, Ji-Young (Laboratory of Cell Engineering & 3-D Structure, Graduate School of Life Sciences and Biotechnology, Korea University)
Lee, Kyung-Eun (Laboratory of Cell Engineering & 3-D Structure, Graduate School of Life Sciences and Biotechnology, Korea University)
Han, Sung-Sik (Laboratory of Cell Engineering & 3-D Structure, Graduate School of Life Sciences and Biotechnology, Korea University)
Publication Information
Applied Microscopy / v.38, no.2, 2008 , pp. 73-79 More about this Journal
Abstract
In Biology, Studies Using Electron Microscopy for making Cell Structure to 3D reconstruction very fast development. Recently, by using Cryo fixation, we can see cell 3D structure without structural change, instead of using chemical fixation which can change cell structure. Before using this technology, we could understand cell structures only in 2D images. But now, through cryo-ET, 3D reconstruction of cell structure without artificial structure changes can be possible and this technology will give us many advantages in Drug delivery and Nanothechnology.
Keywords
Electron tomography; CEMOVIS; 3D reconstruction;
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