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Rhabdomere Formation in Late Pupal Stage of Drosophila melanogaster; Observation Using High-Pressure Freezing and Freeze-Substitution, and High-Voltage Electron Microscopy  

Mun, Ji-Young (Laboratory of Cell Engineering & 3-D Structure, Graduate School of Life Sciences and Biotechnology, Korea University)
Arii, Tatsuo (Section of Brain Structure, Center for Brain Experiment, National Institute for Physiological Sciences, Okazaki National Research Institutes)
Hama, Kiyoshi (Section of Brain Structure, Center for Brain Experiment, National Institute for Physiological Sciences, Okazaki National Research Institutes)
Han, Sung-Sik (Laboratory of Cell Engineering & 3-D Structure, Graduate School of Life Sciences and Biotechnology, Korea University)
Publication Information
Applied Microscopy / v.37, no.1, 2007 , pp. 35-42 More about this Journal
Abstract
The late pupal stage of Drosophila melanogaster occurs immediately before the completion of retinal development, during which the rhabdomere rapidly forms. In this period, the photoreceptor cells were fixed and dehydrated using a high-pressure freezer (HPF) and freeze substitution (FS) technique, which is the most effective in preserving the cell structures, and observed using high-voltage electron microscopy (HVEM) at 1000 KV. The rhabdomere was classified structurally into three types of formation patterns using stereo-tiling image of thick sections. Initially, hexagonal arrays of rhabdomere existed in different angles. In addition, small pieces of rhabdomere could be observed in the cytoplasm of the photoreceptor rolls, which were visible during the profess of rhabdomere formation. In addition, multiple layers of rhabdomere strings were observed. We observed there are at least three types of vesicles related to rhabdomere formation in photoreceptor cells. In addition, it was found that these vesicles initiate the formation of the rhabdomeres during the pupal stage. Collectively, these data suggest that rhabdomeres were mainly formed through vesicles, and that parts of the rhabdomere formed first and then gathered and formed rhabdomeres in the late pupal stage.
Keywords
Drosophila; HVEM; HPF-FS; Rhabdomere;
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