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Ultrastructural Changes of Endoplasmic Reticulum on Hepatocytes by Cyclohexane Injection in Alcohol-pretreated Rats  

Kim, Byung-Ryul (Waterworks Headquaters Daegu Metropolitan City)
Yoon, Chong-Guk (Department of Public Health, Keimyung University)
Cho, Hyun-Gug (Department of Visual Optics, Kyungwoon University)
Publication Information
Applied Microscopy / v.36, no.4, 2006 , pp. 291-297 More about this Journal
Abstract
To evaluate the effects of ingestion of alcoholic drinks on the toxicities of industrial compounds, cyclohexane (CH) was intraperitoneally administrated to rats (1.56g/kg body weight), which had been ingested 15% ethanol for up to 6 weeks,4 times by once a day and every other day. Following the last treatment of ethanol or CH, blood and liver tissues were collected after 4 hours prior to sacrifice of animals. By the injection of CH, liver weight (% of body weight) and xanthine oxidase activity in serum were increased, and glucose-6-phasphatase (G6P) activity in liver was decreased compared to them of control group. The activities of CH metabolizing enzymes, such as cytochrome P450 dependent aniline hydroxylase (CYPdAH) and alcohol dehydrogenase (ADH), were significantly increased by injection of CH, and those activities were the highest in CH-injected group after pretreated with alcohol. Ultrastructurally. both of alcohol treatment and CH injection induced transforming into the smooth-endoplasmic reticulum from rough-endoplasmic reticulum, the those rate was the highest in case of CH-injection after pretreated with alcohol. From these results, it is suggested that alcohol intake on a level without alcoholic degeneration of hepatocytes could enhance the CH metabolism of liver.
Keywords
Alcohol; Cyclohexane; Endoplasmic reticulum;
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1 Hetu C, Dumont A, Joly JG: Effect of chronic ethanol administration in bromobenzene liver toxicity in the rat. Toxicol Appl Pharmaco 67(2) : 66-77, 1983
2 Leiber CS: Hepatic, metabolic and toxic effects of ethanol: 1991 update. Alcoholism Clin Exp Res 15 : 573-592, 1991   DOI   ScienceOn
3 Nordmann R, Ribiere C, Rouach H: Implication of free radical mechanisms in ethanol-induced cellular injury. Free Radical Biol Med 12 : 219-240, 1992   DOI   ScienceOn
4 Schaff Z, Lapis K: Injury by drugs and toxins. In: Electron Microscopy in Human Disease, Vol. 8. The Liver, edited by Johannessen JV, McGraw-Hill, New York, pp. 89-116, 1979
5 Stott WT, Quast JF, Watanabe PG: The pharmacokinetics and macromolecular interactions of trichloroethylene in mice and rats. Toxicol Appl Pharmacol 62 : 137-152, 1982   DOI   ScienceOn
6 Perbellini L, Brugnone F: Lung uptake and metabolism of cyclohexane in shoe factory workers. Int Arch Occup Environ Health 45 : 261-269, 1980   DOI
7 Reinke LA, Moyer MJ, Notley KA: Diminished rates of glucuronidation and sulation in perfused rat liver after chronic ethanol administration. Biochem Phamacol 35 : 439-447, 1986   DOI   ScienceOn
8 Rothschild MA, Oratz M, Schreiber SS: Influence of ethanol or albumin metabolism. In: Alcohol and the Liver, edited by Fisher MM and Rankin JB, Plenum Press, New York, pp. 79-100, 1977
9 Leiber CS: Ethanol metabolism, cirrhosis and alcoholism. Clinica Chemica Acta 257(1) : 59-84, 1997   DOI   ScienceOn
10 Smith AC, Freeman RW, Harbison RD: Ethanol enhancement of cocaine-induced hepatotoxicity. Biochem Pharmacol 30 : 453-458, 1980   DOI   ScienceOn
11 Hasumura Y, Tescke R, Lieber CS: Increased carbon tetrachloride hepatotoxicity and its metabolism after chronic ethanol consumption. Gastroenterol 66(3) : 415-422, 1974
12 Bergmeyer HU, Gawehn K, Grassle M: Methods of enzymatic analysis. Alcohol dehydrogenase. N.Y., Academic Press. Vol. 1, 1974
13 Bidlack WR, Lowly GL: Multiple drug metabolism: p-nitroanisole reversal of aceton enhanced aniline hydroxylation. Biochem Pharmacol 31(3) : 311-317, 1982   DOI   ScienceOn
14 Kim BR, Yoon CG: Effect of cyclohexane treatment on the liver cytochrome P-450 dependent aniline hydroxylase activity in alcohol-pretreated rats. Kor J Env Hlth 29 : 23-28, 2003. (Korean)
15 Bernard AM, de Russis R, Normand JC, Lauwerys RR: Evaluation of the subacute nephrotoxicity of cyclohexane and other industrial solvents in the female Sprague-Dawley rat. Toxixol Letters 45 : 271-280, 1989   DOI   ScienceOn
16 Szasz G: A kinetic photometric method for serum gammaglutamyl transpeptidase. Clinical Chemistry 15 : 124-136, 1969
17 Mutti A, Falzoi M, Lucertini S, Cavatorta A, Franchini I, Pedroni C: Absorption and alveolar excretion of cyclohexane in workers in a shoe factory. J Appl Toxicol 1(4) : 220-223, 1981   DOI
18 Reitman S, Frankel S: A colorimetric method for the determination of serum glutamic oxaloacetic and glutamic pyruvic transaminase. Am J Clin Pathol 28 : 58-63, 1957
19 Nielsen VG, Tan S, Weinbroum A, McCammon AT, Samuelson PN, Gelman S, Parks DA: Lung injury after hepatoenteric ischemia-reperfusion: role of xanthine oxidase. Am J Respir Crit Care Med 154 : 1364-1369, 1996   DOI   ScienceOn
20 Seedorf L, Olsen E: Exposure to organic solvents-I. A survey on the use of solvents. The Annuals of Occupational Hygiene 34(4) : 371-378, 1990   DOI   ScienceOn
21 Nakajima T, Wang RS, Elovaara E, Park SS, Gelboin HV, Vainio H: Cytochrome P450-related differences between rats and mice in the metabolism of benzene, toluene and trichloroethylene in liver microsomes. Biochem Pharmacol 45 : 1079-1085, 1993   DOI   ScienceOn
22 Sato A, Nakajima T, Koyama Y: Dose related effects of single dose of ethanol on the metabolism in rat liver of some aromatic and chlorinated hydrocarbons. Toxicol Appl Pharmacol 60 : 8-15, 1981   DOI   ScienceOn
23 Liira J, Riihimaki V, Engstrom K: Effects of ethanol on the kinetics of methyl ethyl ketone in man. British J Industrial Med 47(5) : 325-330, 1990
24 Yoon CG: A modified colormetric assay for xanthine oxidase in rat liver extracts. Keimyung Res J 2 : 295-308, 1984. (Korean)
25 Yasugi T, Kawai T, Mizunuma K, Kishi R, Harabuchi I, Yuasa J, Eguchi T, Sugimoto R, Seiji K, Ikeda M: Exposure monitoring and health effect studies of workers occupationally exposed to cyclohexane vapor. Int Arch Occup Environ Health 65 : 343-350, 1994   DOI