Browse > Article

Increased Protein of the Secretory Leukocyte Pretense Inhibitor (SLPI) and the Expression of Growth Factors in NIH3T3 Cells by LPS Stimulation  

Lee, Sang-Hwa (Department of Periodontology, College of Dentistry, Chosun University)
Choi, Baik-Dong (Department of Oral Histology, College of Dentistry, Chosun University)
Jeong, Soon-Jeong (Department of Oral Histology, College of Dentistry, Chosun University)
Jang, Hyun-Seon (Department of Periodontology, College of Dentistry, Chosun University)
Kim, Byung-Ock (Department of Periodontology, College of Dentistry, Chosun University)
Lim, Do-Seon (Department of Dental Hygiene, Seoul Health College)
Park, Joo-Cheol (Department of Oral Histology, College of Dentistry, Chosun University)
Wang, Guan-Lin (Department of Oral Histology, College of Dentistry, Chosun University)
Jeong, Moon-Jin (Department of Oral Histology, College of Dentistry, Chosun University)
Publication Information
Applied Microscopy / v.36, no.3, 2006 , pp. 165-172 More about this Journal
Abstract
Secretory leukocyte protease inhibitor (SLPI) involves tissue protection against the destructive action of neutrophil elastase at the site of inflammation. Several studies on new functions of SLPI have demonstrated that SLPI may play a primary role in innate immunity than protease inhibitor, To identify the function of SLPI by lipopolysaccharide (LPS) stimulation in the embryonic fibroblast (NIH3T3) cells. we studied the expression of SLPI compared to other growth factors involving the LPS treatment. To address this, we performed the reverse transcriptase polymerase chain reaction (RT-PCR) and Western blots for the detection of mRNA and protein expression of the SLPI and some growth factors such as VEGF. bFGF, and PDGF-BB after LPS stimulation. NIH3T3 cells were exposed 100 ng/mL Escherichia coli LPS for 30min, 60min, 90min, 24h, and 48h, respectively. The result of RT-PCR showed that SLPI and VEGF mRNA was expressed strongly in NIH3T3 without related to LPS stimulation. mRNA of bFGF was weakly expressed such as the expression of the control. PDGF mRNA expression gradually increased follows at time course. However, SLPI protein level was increased in lysates and culture medium by LPS stimulation. Phase contrast microscopic and scanning electron microscopic observation showed that the increased cell number and cytoplasmic enlargement of the NIH3T3 cells. Therefore, it suggests that the LPS upregulates SLPI expression in NIH3T3 cells. Moreover, secreted SLPI may stimulate cell proliferation and migration.
Keywords
Growth factors; Lipopolysaccharide; NIH3T3; Secretory leukocyte protease inhibitor (SLPI);
Citations & Related Records
연도 인용수 순위
  • Reference
1 Abe T, Kobayashi N, Yoshimura K, Trapnell BC, Kim H, Hubbard RC, Brewer MT, Thompson RC, Crystal RG: Expression of the secretory leukoprotease inhibitor gene in epithelial cells. J Clin Invest 87 : 2207-2215, 1991   DOI
2 Albert B, Johnson A, Lewis J, Martin R, Roberts K, Walter P : The molecular biology of The cell 4rd edn pp. 1300-1301, 2002
3 Fritz H: Human mucus proteinase inhibitor (human MPI). Human seminal inhibitor I (HUSI-I), antileukoprotease (ALP), secretory leukocyte protease inhibitor (SLPI). Biol Chem Hoppe-Seyler 369 : 79-82, 1988
4 Jin F, Carl N, Danuta R, Aihao D: Secretory leukocyte protease inhibitor: A macrophage product induced by and antagonistic to bacterial lipopolysaccharide. Cell 48 : 417-426, 1997   DOI   ScienceOn
5 Kaoru T, Teruo K, Masayasu N: Lipopolysaccharide (LPS)-induced IL-6 production by embryonic fibroblasts isolated and cloned from LPS-responsive and LPS-hyporesponsive mice. Molecular Immunology 34 : 16-17, 1997
6 Lavnikova N, Laskin DL: Unique patterns of regulation of nitric oxide production in fibroblasts. J Leukoc Biol 58 : 451-458, 1995   DOI
7 Love RM, Jenkinson HF: Invasion of dental tubules by oral bacteria. Crit Rev Oral Biol Med 13(2) : 171-183, 2002   DOI
8 Pinheiro ML, Feres-Filho EJ, Graves DT, Takiya CM, Elsas MI, Elsas PP, Luz RA: Quantification and localization of platelet-derived growth factor in gingiva of periodontitis patients. J Periodontol 74 : 323-328, 2003   DOI   ScienceOn
9 Laurie RG, Audrey LA, Diane CS: Construction, non-denaturing affinity purification, and characterization of baculovirally expressed human secretory leukocyte protease inhibitor. Protein Expression and Purification 26 : 179-186, 2002   DOI   ScienceOn
10 Zhang D, Simmen RC, Michel FJ, Zhao G, Vale-Cruz D, Simmen FA: Secretory leukocyte protease inhibitor mediates proliferation of human endometrial epithelial cells by positive and negative regulation of growth-associated genes. J Biol Chem 277 : 29999-30009, 2002   DOI   ScienceOn
11 Coin PG, Lindroos PM, Bird GS, Osornio-Vargas AR, Roggli VL, Bonner JC: Lipopolysaccharide up-regulates plateletderived growth factor (PDGF) alpha-receptor expression in rat lung myofibroblasts and enhances response to all PDGF isoforms. J Immunol 156 : 4797-4806, 1996
12 Prochnau D, Rodel J, Hartmann M, Straube E, Figulla HR: Growth factor production in human endothelial cells after Chlamydia pneumoniae infection. Int J Med Microbiol 294 : 53-57, 2004   DOI   ScienceOn
13 Nick D, Gholamreza HG, Jing Z, Lea B, Patrick de B, Hilde R: Secretory leukocyte protease inhibitor promotes the tumorigenic and metastatic potential of cancer cells. PNAS 100 : 5778-5782, 2003   DOI   ScienceOn
14 Ohlsson K, Bergenfeldt M, Bjork P: Functional studies of human secretory leukocyte protease inhibitor. Adv Exp Med Biol 240 : 123-131, 1988
15 Stetler G, Brewer MT, Thompson RC: Isolation and sequence of a human gene encoding a potent inhibitor of leukocyte proteases. Nucleic Acids Res 14 : 7883-7896, 1986   DOI
16 Thompson RC, Ohlsson K: Isolation, properties, and complete amino acid sequence of human secretory leukocyte protease inhibitor, a potent inhibitor of leukocyte elastase. Proc Natl Acad Sci U. S. A. 83 : 6692-6696, 1986
17 Slavin, J: Fibroblast growth factors; At the heart of angiogenesis. Cell Biol Int 19 : 431-444, 1995   DOI   ScienceOn
18 Botero TM, Mantellini MG, Song W, Hanks CT, Nor JE: Effect of lipopolysaccharides on vascular endothelial growth factor expression in mouse pulp cells and macrophage. Eur J Oral Sci 111 : 228-234, 2003   DOI   ScienceOn
19 Jeffrey RJ, Seed MP, Kircher CH, Willoughby DA, Winkler JD: The codependence of angiogenesis and chronic inflammation. FASEB J 11 : 457-465, 1997   DOI
20 Bartold PM, Narayanan AS, Page RC: Platelet-derived growth factor reduces the inhibitory effects of lipopolysaccharide on gingival fibroblast proliferation. J Periodontal Res 27 : 499-505, 1992   DOI
21 Ross HM, Romrell LJ, Kaye GI: Histology: a text and atlas, 3rd edn, pp. 107-110, 1995
22 Leung DW, Cachianes G., Kuang WJ, Goeddel DV, Ferrara N: Vascular endothelial growth factor is a secreted angiogenic mitogen. Science 246 : 1306-1309, 1989   DOI
23 Kumagai Y, Yagishita H, Yajima A, Okamoto T, Konishi K: Molecular mechanism for connective tissue destruction by dipeptidyl aminopeptidase IV produced by the periodontal pathogen Porphyromonas gingivalis. Infect Immun 73 : 2655-2664, 2005   DOI   ScienceOn
24 Sunderkotter C, Steinbrink K, Goebeler M, Bhardwaj R, Sorg C: Macrophages and angiogenesis. J Leuk Biol 55 : 410-422, 1994   DOI