Browse > Article

Germanium Increases Alveolar Macrophage Engulfment of Apoptotic Neutrophils in Acute Lung Injury Induced by Endotoxin  

Cho, Hyun-Gug (Department of Visual Optics, Kyungwoon University)
Publication Information
Applied Microscopy / v.35, no.4, 2005 , pp. 84-90 More about this Journal
Abstract
Neutrophils that influx into the alveolar spaces from circulatory bloods play a important role in pathogenesis of acute lung injury. During the acute inflammatory phase, in order to investigate the acceleration of macrophage phagocytosis to the neutrophils is able to reduce the neutrophil-derived acute lung injury, endotoxemia was induced by insufflation of lipopolysaccharide intratracheally and organic germanium was injected intraperitoneally after endotoxin treatment. At 5 h after endotoxin treatment, lung weight and BAL protein concentration are significantly increased (p<0.001) compared to sham, and that was remarkedly decreased (p<0.001, p<0.01) by injection of germanium. In addition germanium treatment resulted to decreased the number of alveolar PMNs and to increase the percentage of engulfed neutrophils by alveolar macrophages. These observations indicate that organic germanium may have a role of reduction to neutrophil-derived acute lung injury in endotoxemia.
Keywords
Acute lung injury; Germanium; Macrophage engulfment;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Cox GJ, Crossley J, Xing Z: Macrophage engulfment of apoptotic neutrophils contributes to the resolution of acute pulmonary inflammation in vivo. Am J Respir Cell Mol Biol 12 : 232, 1995   DOI   ScienceOn
2 Delclaux C, Rezaiguia-Delclaux S, Delacourt C, Brun- Buisson C, Lafuma C, Harf A: Alveolar neutrophils in endotoxin-induced and bacteria induced acute lung injury in rat. Am J Physiol 273 : 104-112, 1997
3 DiMartino MJ: Antiarthritic and immunoregularory activity of apirogermanium. J Pharmacol Exp Ther 236(1) : 103-110, 1986
4 Lee YM, Hybertson BM, Terada LS, Repine AJ, Cho HG, Repine JE: Mepacrine decreases lung leak in rats given interleukin-1 intratracheally. Am J Respir Crit Care Med 155 : 1624-1628, 1997   DOI   ScienceOn
5 Matute-Bello G, Liles WC, Radella F, Steinberg KP, Ruzinski JT, Hudson LD, Martin TR: Modulation of neutrophil apoptosis by glanulocyte colony-stimulating factor and glanulocyte/macrophage colony-stimulating factor during the course of acute respiratory distress syndrome. Crit Care Med 28; 1, 2000   DOI   ScienceOn
6 Tsuyuki S, Bertrand C, Erard F, Trifilieff A, Tsuyuki J, Wesp M, Anderson GP, Coyle AJ: Activation of the Fas receptor on lung eosinophils leads to apoptosis and the resolution of eosinophilic inflammation of the airways. J Clin Invest 96 : 2924, 1995   DOI   PUBMED   ScienceOn
7 Kupfner JG, Arcaroli JJ, Yum HK, Nadler SG, Yang KY, Abraham E: Role of NF-${\kappa}B$ in endotoxemia-induced alteration of lung neutrophil apoptosis. J Immunol 167(12) : 7044-7051, 2001
8 Parsey MV, Kaneko D, Shenkar R, Abraham E: Neutrophil apoptosis in the lung after hemorrhage or endotoxemia: apoptosis and migration are independent of IL-$1{\beta}$. Clin Immunol 91 : 219, 1999   DOI   ScienceOn
9 Savill JS, Wyllie AH, Henson JE, Walport ME, Henson PM, Haslett C: Macrophage phagocytosis of aging neutrophils in inflammation: programmed cell death in the neutrophil leads to its recognition by macrophages. J Clin Invest 83 : 865, 1989   DOI   PUBMED   ScienceOn
10 Lee YM, Hybertson BM, Cho HG, Repine JE: Platelet activating- factor induces lung inflammation and leak in rats: hydrogen peroxide production along neutrophil-lung endothelial cell interfaces. J Lab Clin Med 140 : 312-319, 2002   DOI   ScienceOn
11 Sasaki K, Ishikawa M, Monma K, Takanayaki G: Effect of carboxyethylgermanium sesquioxide (Ge-132) on the acute inflammation and
12 Suzuki F, Brutkiewicz RR, Pollard RB: Cooperation of lymphokine (s) and macrophages in expression of antitumor activity of carboxyethylgermanium (Ge-132). Antitumor Res 62(2) : 177-182, 1986
13 Lee YM, Park Y, Kim T, Cho HG, Lee YJ, Repine JE: Effect of the inhibition of phospholipase A2 in generation of free radicals in intestinal ischemia/reperfusion induced acute lung injury. Kor J Physiol Pharmacol 3 : 263-273, 1999. (Korean)
14 Aso H, Suzuki F, Yamaguchi T, Hayashi Y, Ebina T, Ishida N: Induction of interferone and activation of NK cells and macrophages in mice by oral administration of Ge 132, and organic germanium compound. Microbiol Immunol 29(1) : 65-74, 1985
15 Morimoto K, Amano H, Sonoda F, Baba M, Senda M, Yoshimine H, Yamamoto H, Ii T, Nakatake T: Alveolar macrophages that phagocytose apoptotic neutrophils produce hepatocyte growth factor during bacterial pneumonia in mice. Am J Respir Cell Mol Biol 24(5) : 608-615, 2001   DOI   ScienceOn
16 Savill JS, Smith J, Ren Y, Sarraf C, Abbott F, Rees AJ: Glomerular mesengial cells and inflammatory macrophages ingest neutrophils undergoing apoptosis. Kidney Int 42 : 924, 1992   DOI   ScienceOn
17 Brown RE, Jarvis KL, Hyland KJ: Protein measurement using bicinconinic acid: elimination of interfering substance. Anal Biochem 102 : 1152-1160, 1989
18 Newman SL, Henson JE, Henson PM: Phagocytosis of senescent neutrophils by human monocyte-derived macrophages and rabbit inflammatory macrophages. J Exp Med 156 : 430, 1982   DOI   ScienceOn
19 Sugiyama S, Okada Y, Sukhova GK, Virmani R, Heinecke JW, Libby P: Macrophage myeloperoxidase regulation by glanulocyte macrophage colony-stimulating factor in human atherosclerosis and implications in acute coronary symdromes. Am J Pathol 158(3) : 879-891, 2001   DOI   ScienceOn
20 Dunican AL, Leuenroth SJ, Grutkoski P, Ayala A, Simms HH: TNFalpha-induced suppression of PMN apoptosis is mediated through interleukin-8 production. Shock 14(3) : 284-289, 2000   DOI   ScienceOn