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TEM Sample Preparation for Cultured Neurons on a Glass Coverslip  

Oh, Hyun-Woo (Korea Research Institute of Bioscience and Biotechnology)
Park, Ho-Yong (Korea Research Institute of Bioscience and Biotechnology)
Publication Information
Applied Microscopy / v.35, no.4, 2005 , pp. 10-15 More about this Journal
Abstract
Cultured neurons from Drosophila brain on a glass coverslip to understand the structural basis of synapse were prepared for TEM observations. Neurons on a coverslip were fixed, dehydrated and embedded in Epon without separating from coverslip. After polymerization, the block was placed in 49% hydrofluoric acid to remove the coverslip. The block was examined under a light microscope to select exact neurons, then trimmed and sectioned for TEM observation.
Keywords
Cell culture; Glass coverslip; Hydrofluoric acid; Synapse;
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1 Pierce JP, Lewin GR: An ultrastructural size principle. Neuroscience 58 : 441-446, 1994   DOI   ScienceOn
2 Redman S: Quantal analysis of synaptic potentials in neurons of the central nervous system. Physiol Rev 70 : 165-198, 1990
3 Pierce JP, Mendell LM: Quantitative ultrastructure of la boutons in the ventral horn: scaling and positional relationships. J Neurosci 13 : 4748-4763, 1993
4 Kartz B: The release of neural transmitter substances. Liverpool, England: Liverpool University, 1969
5 Betz WJ, Bewick GS: Optical analysis of synaptic vesicle recycling at the frog neuromuscular junction. Science 255 : 200-203, 1992   DOI   PUBMED
6 Schikorski T, Stevens CF: Quantitative ultrastructural analysis of hippocampal excitatory synapses. J Neurosci 17 : 5858-5867, 1997
7 Fukuda T, Kosaka T: Gap junctions linking the dendritic network of gabaergic interneurons in the hippocampus. J Neurosci 20 : 1519-1528, 2000
8 Luse SA: Electron microscope observations of the central nervous system. J Biophys Biochem Cytol 2 : 531-542, 1956   DOI
9 Korneliussen H: Elongated profiles of synaptic vesicles in motor endplates. Morphological effects of fixative variations. J Neurocytol 1 : 279-296, 1972   DOI   PUBMED
10 Fukuda T, Kosaka T: Ultrastructural study of gap junctions between dendrites of parvalbumin-containing gabaergic neurons in various neocortical areas of the adult rat. Neuroscience 120 : 5-20. 2003   DOI   ScienceOn
11 Nakajima Y: Fine structure of the synaptic endings on the mauthner cell of the gold fish. J Comp Neurol 156 : 379-402, 1974
12 Palay SL, Palade GE: The fine structure of neurons. J Biophys Biochem Cytol 1 : 69-88, 1955   DOI
13 Ryan TA, Ziv NE, Smith SJ: Pointiation of evoked vesicle turnover at individually resolved synaptic boutons. Neuron 17 : 125-134, 1996   DOI   ScienceOn
14 Walmsley B, Wieniawa-Narkiewicz E, Nicol MJ: The ultrastructural basis for synaptic transmission between primary muscle afferents and neurons in Clarke's column of the cat. J Neurosci 5:2095-2106, 1985
15 Harris KM, Sultan P: Variation in the number, location and size of synaptic vesicles provides an anatomical basis for the nonuniform probability of release at hippocampal CA1 synapses. Neuropharmacology 34 : 1387-1395, 1995   DOI   ScienceOn
16 Betz WJ, Mao F, Bewick GS: Activity-dependent fluorescent staining and destaining of living vertebrate motor nerve terminals. J Neurosci 12 : 363-375, 1992