Browse > Article

Effect of PLA2 Inhibitor Rutin on Endotoxin-Induced Acute Lung Injury  

Kim, Seong-Eun (Department of Biology, Yeungnam University)
Lee, Young-Man (Department of Physiology, Catholic University of Daegu)
Park, Won-Hark (Department of Biology, Yeungnam University)
Publication Information
Applied Microscopy / v.34, no.1, 2004 , pp. 31-42 More about this Journal
Abstract
Acute respiratory distress syndrome (ARDS) is a kind of acute lung injury characterized by inflammatory disruption of alveolar-capillary barrier and notorious for its high mortality. Neutrophils cause cell damage through the production of free radicals, inflammatory mediators, and proteases in ARDS. $PLA_2$ might serve a primary regulatory role in the activation of neutrophils. This present study was performed to elucidate the effect of rutin known as $PLA_2$ inhibitor on ARDS induced by endotoxin. Endotoxin had increased lung myeloperoxidase (MPO) activity, BAL (bronchoalveolar lavage) protein content, numbers of neutrophils in BALF (bronchoalveolar lavage fluid) compared with those of control rat (p<0.001). In addition, histological evidence of lung injury was correlated with neutrophil influx into alveolar space and cerrous perhydroxide granules were found in lining of endothelial cell, alveolar type I, II cells. In contrast, pretreated group of rutin had significantly decreased all of the parameters (p<0.001). These data suggest that inhibition of $PLA_2$ is one step approach that block the process of ARDS. Accordingly, we conclude that rutin can be used as the prophylactic agent for ARDS on the bases of these experimental results.
Keywords
Acute lung injury; ARDS; Endotoxin; Neutrophils; Rutin;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Brown RE, Jarvis KL, Hyland KJ: Protein measurement using bicinchoninic acid: elimination of interfering substance. Anal Biochem 180 : 136 139, 1989   DOI   ScienceOn
2 Chang Joseph, Musser JH, McGregor Herb: Phospholipase $A_2$ : Function and Pharmacological regulation. Biochem pharmacoI 36(15): 2429 2436, 1987   DOI   ScienceOn
3 Schoenberg MH, Beger HG: Reperfusion injury after intestinal ischemia. Crit Care Med 21(9) : 1376 86, 1993   DOI   ScienceOn
4 Villar J and Slutsky AS: The incidence of the adult respiratory distress syndrome. Am Rev Respir Dis 14 : 814 6, 1989
5 Ware LB, Metthay MA: The acute respiratory distress syndrome. N Eng J Med 342 : 1334 1349, 2000   DOI   ScienceOn
6 Weiland JE, Davis WB, Holter JF, Mohammed JR, Dorinsky PM, Gadek JE: Lung neutrophils in the adult respiratory distress syndrome. Clinical and pathophysiologic significance. Am Rev Respir Dis 133(2) : 218 25, 1986
7 Rinaldo JE, Rogers RM: Adult respiratory distress syndrome: changing concepts of lung injury and repair. N Engl J Med 306 (15) : 900 9, 1982   DOI   ScienceOn
8 Goldblum SE, Wu KM, Jay M: Lung myeloperoxidase as a measurement of Ieukostasis in rabbits. J Appl Physiol 59 : 1978 1985, 1985
9 Vadas P, Browning J, Edelson J, Pruzanski W: Extracellular phospholipase $A_2$ expression and inflammation: the relationship with associated disease states. Journal of Lipid Mediators8: 1 30, 1993
10 Glauser MP: Pathophysiologic basis of sepsis: Considerations for future strategies of intervention. Crit Care Med 28(9 Suppl.): S4 S8,2000   DOI   PUBMED   ScienceOn
11 Vadas P, Hay JB: Involvement of circulating phospholipase $A_2$ in the pathogenesis of the hemodynamic changes in endotoxin shock. Can J Physiol PharmacoI 61(6) : 561 6, 1983   DOI   ScienceOn
12 Connelly KG, Repine JE: Markers for predicting the development of acute respiratory distress syndrome. Annu Rev Med 48 : 429 45, 1997   DOI   ScienceOn
13 Ashbaugh DG, Bigelow DG: Acute respiratory distress in adults. Lancet 2 : 319 23, 1967
14 Wiener Kronish JP, Gropper MA, Matthay MA: The adult respiratory distress syndrome: definition and prognosis, pathogenesis and treatment. Br J Anaesth 65(1) : 107 29, 1990   DOI   ScienceOn
15 Aldridge AJ: Role of the neutrophil in septic shock and the adult respiratory distress syndrome. Eur J Surg 168(4) : 204 14, 2002   DOI   ScienceOn
16 Baur M, Schmid TO, Landauer B: Role of phospholipase $A_2$ in multiorgan failure with special reference to ARDS and acute renal failure (ARF). Klin Wochenschr, 67(3) : 196 202, 1989   DOI
17 Lee YM, Hybertson BM, Cho HG, Terada LS, Cho O, Repine AJ, Repine JE: Platelet activating factor contributes to acute lung leak in rats given interleukin 1 intratracheally. Am J Physiol Lung Cell Mol Physiol 279(1) : L75 80, 2000   DOI
18 Lee YM, Hybertson BM, Terada LS, Repine AJ, Cho HG, Repine JE: Mepacrine decreases lung leak in rats given interleukin 1 intratracheally. Am J Respir Crit Care Med 155(5) : 1624 8, 1997   DOI   ScienceOn
19 Terada LS, Dormish JJ, Shanley PF, Leff JA, Anderson BO, Repine JE: Circulating xanthine oxidase mediates lung neutrophil sequestration after intestinal ischemia reperfusion. Am J Physiol 263(3 Pt 1): L394 401,1992
20 Hobson J, Wright J, Churg A: Histochemical evidence for generation of active oxygen species on the apical surface of cigarette smoke exposed tracheal explants. Am J Pathol 139 : 573 580, 1993
21 Bomalaski JS, Baker D, Resurreccion NV, Clark MA: Rheumatoid arthritis synovial fluid phospholipase $A_2$ activating protein (PLAP) stimulates human neutrophil degranulation and superoxide ion production. Agents Actions 27(3 4) : 425 7, 1989   DOI   ScienceOn
22 Bannerman DD, Goldblum SE: Mechanisms of bacterial lipopolysaccharide induced endothelial apoptosis. Am J Physiol Lung Cell Mol Physiol 284(6) : L899 914, 2003
23 Downey GP: Regulation of neutrophil activation in acute lung injury. Chest 116(1 suppl) : 46S 54S, 1999   DOI   ScienceOn
24 Durham SK, Selig WM: Phospholipase $A_2$ induced pathophysiologic changes in the guinea pig lung. Am J Pathol 136(6) : 1283 91, 1990