Browse > Article

The Role of Protein Kinase C in the Cardiac Injury Induced by Skin Burn  

Moon, Hye-Jung (Department of Biology, Yeungnam University)
Cho, Hyun-Gug (Department of Visual Optics, Kyungwoon University)
Park, Won-Hark (Department of Biology, Yeungnam University)
Publication Information
Applied Microscopy / v.33, no.4, 2003 , pp. 299-313 More about this Journal
Abstract
The aim of the present study was to assess the role of protein kinase C (PKC) in the development of cardiac injury following scald burn. Sprague-Dawley rats were induced a scald burn a 15% total body surface area. Phorbol 12-myristate 13-acetate (PMA, 2 mg/kg) and bisindolylmaleimide (BIS, 0.05 mg/kg) were immediately administered i.p. after burn injury. 5 h and 24 h later, heart was removed and examined biochemical assay, ultrastructural changes and stereological analysis. The activity of serum aspartate aminotransferase was significantly increased at 5h (p<0.01) and 5h+BIS (p<0.001) after burn compared with that of control. The activity of serum creatinine was significantly decreased in PMA-treated groups after burn compared with postburn 5 h. PMA caused a decrease in MPO activity and induced wavy fibers in cardiac myocytes at postburn 5 and 24h. BIS induced contraction band, separation of intercalated disk and abnormal mitochondria in cardiac myocytes at postburn 5 and 24h. In stereological analysis, treatment of rats with PMA increased volume density of myofibril and mitochondria compared with postburn 5 and 24h. Our data suggest that the activation of PKC in scald burned heart decreases inflammation and protects the myocardium.
Keywords
Burn injury; Inflammation; Protein kinase C;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Battaini F: Protein kinase C isoforms as therapeutic targets in nervous system disease states. Pharmacol Res 44(5) : 353 361,2001   DOI   ScienceOn
2 Cain BS, Meldrum DR, Harken AH: Protein kinase C in normal and pathologic myocardial states, J Surg Res 81 : 249 259,1999   DOI   ScienceOn
3 Cain BS, Meldrwn DR, Meng X, Shames BD, Banerjee A, Harken AH: Calcium preconditioning in hmuan myocardi-um, Ann Thorac Surg 65: 1065 1070,1998   DOI   ScienceOn
4 Chi L, Yang Z, Huan Y: Effects of abnonnal distribution of calcium on impairment of myocardial mechanics in the early stage of thennal injury, Zhonghua Zheng Xing Shao ShangWai KeZaZhi 14(1): 33 36,1998
5 Clerk A: Death by protein kinase C inhibitor: astressful event. J MoI Cell Cardiol 33:1773 1776, 2001   DOI   PUBMED   ScienceOn
6 Horton JW, Mileski WJ, White DJ, Lipsky P: Monoclonal antibody to intercellular adhesion molecule-1 reduces cardiac contractile dysfunction after bum injury in rabbits, J Surg Res 64 : 49 56, 1996   DOI   ScienceOn
7 Jeschke MG, Low JF, Spies M, Vita R, Hawkins HK, Hern-don DN, Barrow RE: Cell proliferation, apoptosis, NF kappa B expression, enzyme, protein and weight changes in livers of burned rats, Am J Physiol Gastrointest Liver PhysioI 280(6): G1314 1320, 2001   DOI
8 Lasley RD, Noble MA, Mentzer RM: Effects of protein kinase C inhibitors in in situ and isolated ischemic rabbit myocardium, J Mol Cell Cardiol 29 : 3345 3356,1997   DOI   ScienceOn
9 Loud AV, Anversa P, Giacomelli F, Wiener J: Absolute morphometric study of myocardial hypertrophy in experimental hypertension, I, Determination of myocyte size, Lab Invest 38 : 586 596,1978
10 Park WH, Chung HJ, Kim DB: Effect of chlorambucil on cardiac ultrastructure of mouse, Korean J Electron Microscopy 16(1): 47 62, 1989,(Korean)
11 Sciorra VA, Hammond SM, Morris AJ: Potent direct inhibition of mammalian phospholipase Disozymes by calphos-tin c, Biochem 40: 2640 2646,2001   DOI   ScienceOn
12 Venema RC, Kuo JF: Protein kinase C mediated phophorylation oftroponin I and C protein in isolate myocardial cells is associated with inhibition of myofibrillar actomyosin MgATPase, J Biol Chem 268 : 2705 2711, 1993
13 Johnson JA, Gray MO, Che Hong Chen, Mochly Rosen D: A protein kinase C translocation inhibitor as an isozyme selective antagonist of cardiac function, J Biol Chem 271 (40) : 24962 24966, 1996   DOI   PUBMED
14 Horton JW, White J, Maass D: PKC inhibition improves ventricular fWlction after thennal trauma, J Trauma 44(2): 254 264, 1998   DOI   ScienceOn
15 Yang J, Yang Z, Chen F: The measurement of cardiac myosin light chain I in diagnosis of postbum cardiac injury, Zhonghua Zheng Xing Shao Shang Wai Ke Za Zhi 13(1) : 34 36,1997
16 Sener G, Sehirli AO, Satiroglu H, Keyer Uysal M, Ydgen BC: Melatonin improves oxidative organ damage in a rat model ofthermal injury, Burns 28 :419 425,2002   DOI   ScienceOn
17 XiaZF, HortonJW, Tang HT, YangY: Metabolic disorder in myocardial intracellular free calcium after thermal injury, Bums 27 :453 457, 2001
18 Yin Z, Zhou J, Cai S, Wang Z: On line dynamic measurement of blood viscosity, hematocrit and change of blood volwne, Chin J Traumatol 15: 3(2): 102 106,2000
19 Kannazym M, Watson JE, Moffat MP: Mechanisms cardiac depression induced by phorbol myristate acetate in work-ing rat hearts, Br J Pharmacol 100 : 826 830,1990   DOI   ScienceOn
20 Fournier A, Murray AW: Application of phorbol ester to mouse skin causes a rapid and sustained loss of protein kinase C, Nature 330(6150): 767 769,1987   DOI   ScienceOn
21 Merritt JE, Sullivan JA, Tse J, Willcinson SE, Nixon JS: Different sensitivities of neutrophil responses to a selective protein kinase C inhibitor, Ro 31 8425; Redundancy in signal transduction, Cell Signal 9(1) : 53 57,1997   DOI   ScienceOn
22 YOWl YK, LaLonde C, Demling R: The role of mediators in the response to thermal injury, World J Surg 16(1): 30 36, 1992   DOI   ScienceOn
23 Faunce DE, Llanas IN, Patel PJ, Gregory MS, Duffner LA, Kovacs EJ: Neutrophil chemokine production in the skin following scald injury, Bums 25(5): 403 410,1999
24 Ganote CE: Contraction band necrosis and irreversible myocardial injury, J Mol Cell Cardiol 15 : 67 73,1983
25 Goldblum SE, Wu KE, Jay M: Lungg myeloperoxidase as a measurement of leukostasis in rabbits, J Appl Physiol pp, 1978 1985,1985
26 Forbes MS, Sperelakis N: Structures located at the level of the Z bands in mouse ventricular myocardial cells, Tissue Cell 12 :467 489,1980   DOI   ScienceOn
27 Sheeran PW, Maass DL, White DJ, Turbeville TD, Giroir BP, Horton JW: Aspiration pewnonia induced sepsis increases cardiac dysfunction after bum trauma, J Surg Res 76 : 192 199,1998   DOI   ScienceOn
28 Dries DJ, Lorenz K, Kovacs EJ: Differential neutrophil traffic in gut and lung after scald injury, J Bum Care Rehabil 22(3):203 209,2001   DOI   ScienceOn
29 Nishizuka Y: Intracellular signaling by hydrolysis of phospholipids and activation of protein kinase C, Science 258 : 607 613, 1992   DOI   PUBMED
30 Vogt AM, Htun P, Arras M, Podzuweit T, Schaper W: Intramyocardial infusion of tool drugs for the study of molecular mechanisms in ischemic preconditioning, Basic Res Cardiol 91(5): 389 400,1996
31 Pi YQ, Walker JW: Diacylglycerol and fatty acids synergistically increase cardiomyocyte contraction via activation of PKC, Am J Physiol, Heart Circ Physiol 279 : H26 34, 2000   DOI
32 Bogoyevitch MA, Parker Pl, Sugden PH: Characterization of protein kinase C isotype expression in adult rat heart. Protein kinase $C\:\xi$ is a major isotype present and it is activated by phorbol esters, epinephrine, and endothelin. cire Res 72: 757 767, 1993   DOI   ScienceOn
33 Horton JW, White DJ, Maass D, Sanders B, Thompson M, Giroir B: Calcium antagonists improve cardiac mechanical performance after thermal trauma, J Surg Res 87 : 39 50, 1999   DOI   ScienceOn
34 Mochly Rosen O, Gordon AS: Anchoring proteins for protein kinase C: a means for isozyme selectivity, FASEB 12 : 35 42, 1998   DOI
35 Chang J, Musser JH, McGregor H: Phospholipase A2: Function and pharmacological regulation, Biochemical Pharmacology 36(15) : 2429 2436,1987   DOI   ScienceOn
36 Ogita K, Miyamoto Slm Yamaguchi K, Koide H, Fujisawa N, Kikkawa U, Sahara S, Fukami Y, Nishizuka Y: Isolation and characterization of Ii subspecies of protein kinase C from rat brain, Proc Natl Acad Sci USA 89 : 1592 1596, 1992   DOI   ScienceOn
37 Spector: In: Handbook of Biological Data, Philadelphia, PA Sounders, p, 157, 1973, Sugden PH, Bogoyevitch MA: Intracellular signalling through prote in kinase s in the heart, Cardiovasc Res 30: 478 492, 1995   DOI
38 Bogoyevitch MA, Parker Pl, Sugden PH: Characterization of protein kinase C isotype expression in adult rat heart. Protein kinase C e is a major isotype present and it is activated by phorbol esters, epinephrine, and endothelin. cire Res 72: 757 767, 1993   DOI   ScienceOn
39 Cho H, Yown JB, Earm YE, Ho WK: Inhibition of acetylcholine activated $K^+$ current by chelerythrine and bisindolyhnaleimide I in atrial myocytes from mice, Eur J Phar-macol 424: 173 178, 2001   DOI   ScienceOn
40 Noguchi T, Chen Z, Bell SP, Nyland L, LeWinter MM: Activation of PKC decreases myocardial O2 consumption and increases contractile efficiency in rats, Am J Physiol Heart Circ Physiol 281(5) : H2191 2197,2001   DOI
41 Koide H, Ogita K, Kikkawa U, Nishizuka Y: Isolation and characterization of the e subspecies of protein kinase C from rat brain, Proc Natl Acad Sci USA 89 : 1149 1153, 1992   DOI   ScienceOn