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Effect of Porous Membrane on Culture Properties of Blood-Brain Barrier Endothelial Cell  

Lee, Keum-Jeong (Department of Anatomy, College of Medicine, Chung-Ang University)
Cho, Hye-Jin (Department of Anatomy, College of Medicine, Chung-Ang University)
Choi, Hyung-Taek (Department of Anatomy, College of Medicine, Chung-Ang University)
Na, O-Soon (Department of Anatomy, College of Medicine, Chung-Ang University)
Kim, Kyung-Yong (Department of Anatomy, College of Medicine, Chung-Ang University)
Publication Information
Applied Microscopy / v.33, no.4, 2003 , pp. 261-266 More about this Journal
Abstract
The growth patterns of primary culture of bovine brain microvessel endothelial cells (BBMECs) were studied using electron microscopy when grown on $3.0{\mu}m$ and $0.4{\mu}m$ pore Transwell. The capillary fragments and isolated endothelial cells grew on collagen coated culture plate and Transwell membrane. The BBMECs grew only on the upper surface of membrane of $0.4{\mu}m$. But BBMECs on $3.0{\mu}m$ pore membrane migrated through the pore and grew on the opposite side of the membrane. In summary, BBMECs isolated by enzyme digestion could migrate through $3.0{\mu}m$ pore membrane but not through $0.4{\mu}m$ pore membrane. So $0.4{\mu}m$ pore membrane instead of $3{\mu}m$ pore membrane should be used for drug transport experiment or transendothelial electrical resistance measurement.
Keywords
BBB; Endothelial cell; Transwell;
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