Browse > Article

Effect of Porous Membrane on Culture Properties of Blood-Brain Barrier Endothelial Cell  

Lee, Keum-Jeong (Department of Anatomy, College of Medicine, Chung-Ang University)
Cho, Hye-Jin (Department of Anatomy, College of Medicine, Chung-Ang University)
Choi, Hyung-Taek (Department of Anatomy, College of Medicine, Chung-Ang University)
Na, O-Soon (Department of Anatomy, College of Medicine, Chung-Ang University)
Kim, Kyung-Yong (Department of Anatomy, College of Medicine, Chung-Ang University)
Publication Information
Applied Microscopy / v.33, no.4, 2003 , pp. 261-266 More about this Journal
Abstract
The growth patterns of primary culture of bovine brain microvessel endothelial cells (BBMECs) were studied using electron microscopy when grown on $3.0{\mu}m$ and $0.4{\mu}m$ pore Transwell. The capillary fragments and isolated endothelial cells grew on collagen coated culture plate and Transwell membrane. The BBMECs grew only on the upper surface of membrane of $0.4{\mu}m$. But BBMECs on $3.0{\mu}m$ pore membrane migrated through the pore and grew on the opposite side of the membrane. In summary, BBMECs isolated by enzyme digestion could migrate through $3.0{\mu}m$ pore membrane but not through $0.4{\mu}m$ pore membrane. So $0.4{\mu}m$ pore membrane instead of $3{\mu}m$ pore membrane should be used for drug transport experiment or transendothelial electrical resistance measurement.
Keywords
BBB; Endothelial cell; Transwell;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Panula P, Joo F, Rechardt L: Evidence for the presence of viable endothelial cells in culture derived from dissociated rat brain, Experimentia 34: 95 97,1978   DOI   ScienceOn
2 Abbott NJ, Hughes CC, Revest PA, Greenwood J: Development and characterization of a rat brain capillary endothelial culture: towards an in vitro blood brain barrier. J Cell Sci 103 : 23 37, 1992
3 Bowman PD, Ennis SR, Rarey KE, Betz AL, Goldstein GW: Brain microvessel endothelial cells in tissue culture: a model for study of blood brain barrier permeability, Ann Neurol 14: 396 402, 1983   DOI   ScienceOn
4 Greenwood J: Astrocytes, cerebral endthelium and cell culture: the persuit of an in vitro blood brain barrier organ, Ann NY Acad Sci 633: 426 430,1991   DOI
5 Reese TS, Karnovsky MJ: Fine structural localization of a blood brain barrier to exogenous peroxidase, J Cell BioI 34: 207 217,1967   DOI   ScienceOn
6 De Bault LE, Kahn LE, Frommes SP, Cancilla PA: Cerebral microvessels and derived cells in tissue culture: isolation and preliminary characterization, In Vitro 15 : 473 487, 1979   DOI
7 Male OK, Pryce G, Rahman J: Comparison of the immuno-logical properties of rat cerebral and aortic endothelium, J neuroimmunol 30 : 290 295,1990
8 Grabb PA, Gilbert MR: Neoplastic and pharmacological influence on the penneability ofan in vitro blood brain barrier, J Neurosurg 82: 1053 1058,1995   DOI   ScienceOn
9 Audus KL, Borchardt RT: Bovine brain microvessel endothelial cell monolayers as a model system for blood brain barrier. Ann NY Acad Sci 507: 9 18,1987   DOI
10 Szabo CA, Deli MA, Ngo Thi Khue, Joo F: Production of pure primary rat endothelial cell culture; a comparison of different method, Neurobiol 5: 1 16,1997   DOI
11 Shi F, Audus KL: Biochemical characteristics of primary and passaged cultures of primate brain microvessel endothelial cells, Neurochem Res 19 :427 433, 1994   DOI   ScienceOn
12 Bowman PD, Betz AL, Ar D, Wolinsky JS, Penney JB, Shivers RR, Goldstein GW: Primary culture of capillary endothelium from rat brain, In Vitro 17: 353 362,1981   DOI   PUBMED
13 Audus KL, Bartel RL, Hidalgo IJ, Borchardt RT: The use of cultured epithelial and endothelial cells for drug transport and metabolic studies, Pharm Res 7: 435 451,1990   DOI   ScienceOn
14 Demeuse PH, Kerhofs A, Struys Ponsar C, Knoop B, Remade C, Aguilar PH: Compartmentalised co culture of rat brain endothe lial cells and astrocyte: a synergistic mo del to study the blood brain barrier, J Neurosci Methods 121 21 31,2002   DOI   ScienceOn
15 Abbruscato TJ, Davis TP: Combination ofhypoxiaJaglycemia compromises in vitro blood brain barrier integrity. J Pathol Exp Ther 289: 668 675, 1999
16 Eddy EP, Maleef BE, Hart TK, Smith PL: In vitro models to predict blood brain barrier penneability, Adv Drug Del Rev 23 : 185 198,1997   DOI   ScienceOn
17 Arthur FE, Shivers RR, Bowman PD: Astrocyte mediated induction of tight jumctions in brain capillary endothelium: an efficient in vitro model. Dev Brain Res 36 : 155 159, 1987   DOI   ScienceOn
18 Joo F: Endothelial cell of brain and other organ system: some similarities and differences, Progr Neurobiol 48 S35 S37,1993
19 Williams SK, Gillis JF, Matthews MA, Wagner RC, Bitensky MW: Isolation and characterization of brain endothelial cells: Morphology and enzyme activity, J Neurochem 35: 374 381,1980   DOI   PUBMED