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Ultrastructural Localization of ZnT3 and Zinc Ions in the Mouse Choroid Plexus  

Kim, Sung-Joo (Department of Anatomy, Hallym University)
Kim, Yong-Kuk (Department of Anatomy, Hallym University)
Sun, Yuan-Jie (Department of Anatomy, Hallym University)
Kim, Soo-Jin (Department of Biology, Hallym University)
Jeong, Young-Gil (Department of Anatomy, Konyang University,)
Yu, Yun-Cho (Department of Oriental Medicine, Woosuk University)
Jo, Seung-Mook (Department of Anatomy, Hallym University)
Publication Information
Applied Microscopy / v.32, no.4, 2002 , pp. 377-383 More about this Journal
Abstract
We have detected the murine zinc transporter, ZnT3, and zinc ions in the mouse choroid plexus by immunocytochemistry (ICC) and zinc selenium autometallography ($ZnSe^{AMG}$), respectively. BALB/c mice served as experimental animals. Routine floating ABC immunocytochemical procedures were used for the ZnT3 immunocytochemistry, and the mice were injected intraperitoneally (i.p.) with sodium selenide (10 mg/kg) for the zinc selenium autometallography. The choroid plexus showed weak immunoreactivity (Ir) for ZnT3. At high magnification, ZnT3-Ir was seen to be located in the choroid epithelium and the connective tissue of the capillaries. At the EM level, a high electron density of ZnT3-immunoreactivity was restricted to vesicle membranes as well as microvilli in the apical membrane. In contrast, immunostaining of ZnT3 was completely absent in the basolateral plasma membrane and other cell organelles. After silver enhancement, fine $ZnSe^{AMG}$ grains were observed in both the epithelial and endothelial cells of the choroid plexus. Few $ZnSe^{AMG}$ grains present in the cell bodies of the choroid epithelial cells were located in multivesicular bodies. It is striking that very many $ZnSe^{AMG}$ grains were observed in the endothelial cells of the capillaries. These findings establish the choroid plexus as a non-neuronal pool of zinc ions in the brain, although the functional significance of this pool is not clear. The choroid epithelium, however, may play an important role in the transportation of zinc between the CSF and brain tissue.
Keywords
Autometallography; Choroid plexus; Immunocytochemistry; Mouse; Zinc ions; ZnT3;
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