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Effects of Chitosanoligosaccharide on the Mouse Hepatotoxicity Induced by Cadmium  

Yoon, Jung-Sik (Department of Biological Sciences, College of Natural Sciences, Chosun University)
Roh, Young-Bok (Department of Biological Sciences, College of Natural Sciences, Chosun University)
Publication Information
Applied Microscopy / v.32, no.4, 2002 , pp. 361-376 More about this Journal
Abstract
This research was conducted to determine the effects of chitosanoligosaccharide on liver poisoning induced by cadmium (Cd). Three groups of mice were used in this research. The group was only injected with cadmium (5.0 mg/kg; i.p.) (group Cd) and the other group was injected with cadmium and chitosanoligosaccharide (0.5% solution) at the same time (group Cd+Chi). In order to investigate the inhibitory action of chitosanoligosaccharide on liver damage, cadmium concentration in liver tissues and metallothionein (MT) concentration were relatively measured. In addition, histological observations were made to determine the morphologic injury of liver tissues. Cadmium concentration in liver tissues was drastically lower in groups Cd+Chi than in group Cd. MT concentration in liver tissues was lower in group Cd than in groups Cd+Chi. As the result of electron microscopic observation, mitochondria in group Cd showed a severe swelling phenomenon, RER fragment and ribosome dropout. However, in groups Cd+Chi, mitochondria with high electron density were distributed and RER forming a typical lamellae with ribosome was observed. From these results, cadmium toxicity on rat liver tissues could be lessened by chitosanoligosaccharide.
Keywords
Cadmium chloride; Chitosanoligosaccharide; Liver;
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