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Fine Structural Approach of Granular Gland Regeneration after Skin Injury in Bombina orientalis  

Jeong, Moon-Jin (Cellular Immunology Section, Oral Infection & Immunity Branch, National Institute of Dental & Craniofacial Research (NIDCR), NIH)
Lim, Do-Seon (Department of Dental Hygiene, Seoul Health College)
Moon, Myung-Jin (Department of Biological Sciences, Dankook University)
Publication Information
Applied Microscopy / v.32, no.3, 2002 , pp. 275-284 More about this Journal
Abstract
Granular gland regeneration in the toad after dorsal skin wound histologically was examined using scanning and transmission electron microscopy. After cutaneous wounds were induced by excision, animals were maintained in special cages for up to 20 days. In transmission electron microscopy (TEM), newly formed granular gland, though poorly developed, was seen on 4 day after injury. Epithelial cells moved toward apical region of newly formed gland. The cells had smooth surface and were not connected to other cells by desmosomes. Mitochondria rich cell (MRC) possessing long cytoplasmic processes formed a gland cavity and hemidesmosomes were found under the cell processes. Basal cavity of newly formed gland consisted of MRC, pro-granular producing cells (pGPC), and granular producing cell (GPC). Moreover it was observed that xanthophores moved to the base of the epithelial tissue on 10 day after the injury. These cells contained numerous pterinosomes and carotenoid vesicles. Immature pterinosomes were large and carotenoid vesicles were moderately electron dense. On 13 day after the injury, xanthophores contained abundant carotinoid vesicles and lammelated pterinosomes. Iridophores were also observed adjacent the developing xanthophores on 16 day post-injury. These observations indicated that regeneration of granular gland from glandular precursor cells during wound healing and subsequent expansion of the glandular cells might be dependent on maturation and proliferation of these newly formed cells.
Keywords
Granular gland; Pigment cells; Regeneration; Skin injury;
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