Browse > Article
http://dx.doi.org/10.9720/kseg.2013.1.37

Characterization of Water Quality and the Aerobic Bacterial Population in Leachate Derived from Animal Carcass Disposal  

Choi, Nag-Choul (Department of Rural Systems Engineering/Research Institute for Agriculture and Life Sciences, Seoul National University)
Choi, Eun-Ju (Department of Energy and Resource Engineering, Chosun University)
Kim, Bong-Ju (Department of Energy and Resource Engineering, Chosun University)
Park, Jeong-Ann (Department of Rural Systems Engineering/Research Institute for Agriculture and Life Sciences, Seoul National University)
Kim, Song-Bae (Department of Rural Systems Engineering/Research Institute for Agriculture and Life Sciences, Seoul National University)
Park, Cheon-Young (Department of Energy and Resource Engineering, Chosun University)
Publication Information
The Journal of Engineering Geology / v.23, no.1, 2013 , pp. 37-46 More about this Journal
Abstract
Leakage of leachate from animal carcass disposal is a significant issue because disease can easily spread to humans and other livestock. In this study, we analyzed the physicochemical properties of leachate and tested for the presence of aerobic bacteria in leachate using molecular biology methods, for 16 animal carcass disposals in the first stage (after burial for 5 months). Leachate physicochemical analysis revealed higher total coliforms, TOC, $NH^{4+}$, and $NO^{3-}$ concentrations compared with previously published data. In most leachate samples, the concentrations of $NH^{4+}$ and $NO^{3-}$ exceeded the Korean guideline values for drinking water. In 16S rRNA sequence analysis of the distribution of leachate under aerobic conditions, Bacillus pumilus, Lysinibacillus sphaericus, and B. sphaericus were observed with high frequency, whereas no food-poisoning-related bacteria such as B. cereus or Salmonella were detected. The present findings improve our knowledge of the transport of leachate from animal carcass disposal sites through geologic media, and are useful in risk analysis and for subsequent studies.
Keywords
animal carcass disposal; leachate; aerobic bacteria; water quality; molecular biology method;
Citations & Related Records
Times Cited By KSCI : 7  (Citation Analysis)
연도 인용수 순위
1 Ahmed, I., Yokota, A., Yamazoe, A., and Fujiwara. T., 2007, Proposal of Lysinibacillus boronitolerans gen. nov. sp. nov., and transfer of Bacillus fusiformis to Lysinibacillus fusiformis comb. nov. and Bacillus sphaericus to Lysinibacillus sphaericus comb. nov, Int. J. Syst. Evol. Microbol, 57, 1117-1125.   DOI   ScienceOn
2 Davies, R. H. and Wray, C., 1996, Persistence of Salmonella enteritidis in poultry units and poultry food, British Poultry Science 37, 589-596.   DOI   ScienceOn
3 Elizabeth, W. D., Urbina, M., Payne, J., Mula, Mir. S., Darwazeh, H., Dulmage, H. J., and Correa, J. A., 1984, Fate of Bacillus sphaericus 1593 and 2362 Spores Used as Larvicides in the Aquatic Environment, Appl. Environ. Microbiol, 47, 125-129.
4 Hill, J. E., Baiano, J. C. F., and Barnes, A. C., 2009, Isolation of a novel strain of Bacillus pumilus from penaeid shrimp that is inhibitory against marine pathogens, Journal of Fish Diseases, 32(12), 1007-1016.   DOI   ScienceOn
5 Jacobson, K. H., Lee, S. H., McKenzie, D., Benson, C. H., and Pedersen, J. A., 2009, Transport of the Pathogenic Prion Protein through Landfill Materials, Environmental Science and Technology, 43(6), 2022-2028   DOI   ScienceOn
6 Joa, J. H., Lim, H. C., Han, S. G., Chun, S. J., and Suh, J. S., 2007, Characteristics of Bacillus sphaericus PSB-13 as Phosphate Solublizing Bacterium Isolated from Citrus Orchard Soil, Korean Journal of Soil Science and Fertilizer, 40(5), 405-411(In Korean with English abstract).   과학기술학회마을
7 Kang, M. A., Kim, M. S., Choi, B. W., and Sohn, H. Y., 2012, Organic Matter Analysis and Physicochemical Properties of Leachate from a Foot-and-Mouth Disease Landfill Site., Korean Journal of Microbiology and Biotechnology, 40(2), 128-134 (In Korean with English abstract).   과학기술학회마을   DOI   ScienceOn
8 Kim, E. S., Hong, S. W., and Chung, K.S., 2011, Comparative Analysis of Bacterial Diversity in the Intestinal Tract of Earthworm (Eisenia fetida) using DGGE and Pyrosequencing, Korean Journal of Microbiology and Biotechnology, 39(4), 374-381 (In Korean with English abstract).   과학기술학회마을
9 Kim, K. H., Kim, K. R., Kim, H. S., Lee, G. T., and Lee, K. H., 2010, Assessment of Soil and Groundwater Contamination at Two Animal Carcass Disposal Sites, Korean J. Soil Sci. Fert., 43(3), 384-389 (In Korean with English abstract).   과학기술학회마을
10 Kim, W. J., Choi, K. J., and Park, J. S., 2011, An Experimental Study on Water-Purification Properties in Cement Bricks Using Effective Micro-Organisms and Zeolite, Journal of the Korea Concrete Institute, 23(3), 331-338 (In Korean with English abstract).   과학기술학회마을   DOI   ScienceOn
11 MacArthur, A. J. and Milne, J. C., 2002. Leachate Characteristics and Management Requirements Arising from the Foot & Mouth Operations in Scotland. Proceedings Waste 2002 Integrated Waste Management and Pollution Control: Research, Policy and Practice 305-314.
12 Moon, Y. H., Lee, K. B., Kim, Y. J., and Koo, Y. M., 2011, Current Status of EM (Effective Microorganisms) Utilization, Korean Society for Biotechnology and Bioengineering Journal, 26, 365-373 (In Korean with English abstract).   과학기술학회마을   DOI   ScienceOn
13 Mikkola, R., Andersson, M. A., Grigoriev, P., Teplova, V. V., Saris, N. E., Rainey, F. A., and Salkinoja-Salonen, M. S., 2004, Bacillus amyloliquefaciens strains isolated from moisture-damaged buildings produced surfactin and a substance toxic to mammalian cells, Arch. Microbiol, 181, 314-323.   DOI
14 Mikkola, R., Kolari, M., Andersson, M. A., Helin, J., and Salkinoja-Salonen, M. S., 2000, Toxic lactonic lipopeptide from food poisoning isolates of Bacillus licheniformis, Eur. J. Biochem. 267, 4068-4074.   DOI   ScienceOn
15 Miller, L. K., Lingg, A. J., and Bulla L. A. Jr., 1983, Bacterial, Viral, and Fungal Insecticides, Science, 219(4585) 715-721.   DOI   ScienceOn
16 Myers. P. S. and Yousten, A. A., 1980, Localization of a Mosquito-Larval Toxin of Bacillus sphaericus 1593, Appl. Environ. Microbiol. 39, 1205-1212.
17 Park, J. H., 2011, Improvement Plan for the Prevention and Biosecurity of Animal Disease, Journal of Korean Society on Water Quality, 27(3), 371-376 (In Korean with English abstract).
18 Priest, F. G., 1993, Systematics and Ecology of Bacillus, In: Sonenshein AL, Hoch JA, Losick R, editors. Bacillus subtilis and Other Gram-Positive Bacteria: Biochemistry, Physiology, and Molecular Genetics. Washington, D.C.: ASM Press, 3-16.
19 Salkinoja-Salonen, M. S., Vuorio, R., Andersson, M. A., Kämpfer, P., Andersson, M. C., Honkanen-Buzalski, T., and Scoging, A. C., 1999, Toxigenic strains of Bacillus licheniformis related to food poisoning, Appl. Environ. Microbiol. 65, 4637-4645.
20 Stowell, C. and Sazama, K., 2007, Informed consent in blood transfusion and cellular therapies: patients, donors, and research subjects, Amercian Association of Blood Banks, 62, 3.
21 Woo, E. O., Kim, M. J., Son, H. S., Ryu, E. Y., Jeong, S. Y., Son, H. J., Lee, S. J., and Park, G. T., 2007, Production of Keratinolytic Protease by Bacillus pumilus RS7 and Feather Hydrolysate As a Source of Amino Acids, Journal of the Environmental Sciences, 16(10), 1203-1208(In Korean with English abstract).   과학기술학회마을   DOI   ScienceOn
22 Suominen, I., Andersson, M. A., Andersson, M. C., Hallaksela, A. M., Kampfer, P., Rainey, F. A., and Salkinoja- Salonen, M., 2001, Toxic Bacillus pumilus from indoor air, recycled paper pulp, Norway spruce, food poisoning outbreaks and clinical samples, Syst. Appl. Microbiol, 24, 267-276.   DOI   ScienceOn
23 Turnbull, P. C. B., 1999, Definitive identification of Bacillus anthracis-a review, Journal of Applied Microbiology, 87, 237-240.   DOI   ScienceOn
24 UK Department of Health. 2001, A rapid qualitative assessment of possible risks to public health from current foot & mouth disposal options-main report and annexes.
25 Yoon, D. H., Kang, D. W., and Nam, K. W., 2009, The Effect of Yeast(Saccharomyces exiguus SJPAF1) on Odor Emission and Contaminants Reduction in Piggery Slurry, Korean Journal of Environmental Agriculture, 28(1), 47-52 (In Korean with English abstract).   과학기술학회마을   DOI   ScienceOn