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http://dx.doi.org/10.5229/JKES.2020.23.3.73

A Study on the Electrochemical Characteristics of Biosensor with HRP Enzyme Immobilized on SPCE  

Han, Kyoung Ho (Department of Chemical Engineering, Kwangwoon University)
Lee, Dae Hyun (GM Technical center Korea)
Yoon, Do Young (Department of Chemical Engineering, Kwangwoon University)
Choi, Sangil (Department of Environmental Engineering, Kwangwoon University)
Publication Information
Journal of the Korean Electrochemical Society / v.23, no.3, 2020 , pp. 73-80 More about this Journal
Abstract
Fenton oxidation method using hydrogen peroxide is an eco-friendly oxidation method used in water treatment and soil restoration. When removing pollutants by this method, it is quite important to properly regulate the concentration of hydrogen peroxide according to the concentration of the contaminants. In this study, electrochemical biosensors using HRP (horseradish peroxidase) enzymes were manufactured and studies were conducted on the activity of enzymes and the detection characteristics of hydrogen peroxide. HRP were electro deposited with chitosan and AuNP on the working electrode surface of the SPCE (Screen Printed Carbon Electrode). Then, the fixation of enzymes was confirmed using the cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The activity of HRP enzymes was also identified from chronoamperometry (CA) and UV spectroscopy. After immersing the biosensor in PBS solution the current generated from electrodes by titrating hydrogen peroxide was measured from CA analysis. The generated current increased linearly for the concentration of hydrogen peroxide, and a calibration curve was derived that could predict the concentration of hydrogen peroxide from the current.
Keywords
Biosensor; Hydrogen peroxide; Electrochemistry; Horseradish peroxidase;
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Times Cited By KSCI : 2  (Citation Analysis)
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