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http://dx.doi.org/10.5229/JKES.2016.19.4.134

Thrombin Detection with Tetrabromophenolphthalein Ethyl Ester Adsorbed on Aptamer-attached Conductive Polymer  

Chung, Saeromi (Department of Chemistry, Pusan National University)
Noh, Hui-Bog (Department of Chemistry, Pusan National University)
Shim, Yoon-Bo (Department of Chemistry, Pusan National University)
Publication Information
Journal of the Korean Electrochemical Society / v.19, no.4, 2016 , pp. 134-140 More about this Journal
Abstract
An aptamer-based biosensor using a new redox indicator has been examined for the electrochemical detection of thrombin. The aptamer modified primary aliphatic amine was covalently immobilized onto poly-(5,2':5',2"-terthiophene-3'-carboxylic acid) (polyTTCA) layer. Tetrabromophenolphthalein ethyl ester (KTBPE) was interacted to aptamer and used as an electrochemical indicator. Prior to the detection, the oxidation reaction of KTBPE onto aptamer modified layer was also investigated using differential pulse voltammetry. The characterization of the final sensor (KTBPE/aptamer -polyTTCA) was performed by voltammetry, QCM, and ESCA. After binding of thrombin onto KTBPE/aptamer based sensor, the peak signal of KTBPE was gradually decreased. The sensor exhibited a dynamic range between 10.0 and 100.0 nM with the detection limit of $1.0{\pm}0.2nM$.
Keywords
Conducting polymer; Aptamaer; Tetrabromophenolphthalein ethyl ester; Thrombin; Electrochemical sensor;
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