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http://dx.doi.org/10.5229/JKES.2011.14.1.044

Simple Electrochemical Immunosensor for the Detection of Hippuric Acid on the Screen-printed Carbon Electrode Modified Gold Nanoparticles  

Choi, Young-Bong (Department of chemistry, Dankook University)
Tae, Gun-Sik (Department of Life Sciences, Dankook University)
Publication Information
Journal of the Korean Electrochemical Society / v.14, no.1, 2011 , pp. 44-49 More about this Journal
Abstract
This paper describes an electrochemical immunosensor for simple, fast and quantitative detection of a urinary hippuric acid which is one of major biological indicator in toluene-exposed humans. The feature of this electrochemical system for immunoassay of hippuric acid is based on the direct conjugation of ferrocene to a hippuric acid. With the competition between the ferrocene-hippuric acid complex and hippuric acid for binding to the anti-hippuric acid monoclonal antibody coated onto gold nanoparticles, the electrical signals are turned out to be proportional to urinary hippuric acid in the range of 0.01-10 mg/mL, which is enough to be used for the point-of-care. The proposed electrochemical method could extend its applications to detect a wide range of different small molecules of antigens in the health care area.
Keywords
Immunosensor; Hippuric acid; Ferrocene; Point-of-care;
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Times Cited By KSCI : 1  (Citation Analysis)
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