Browse > Article
http://dx.doi.org/10.5229/JKES.2002.5.4.212

A SOD-Based Amperometric Biosensor for Superoxide Ion  

Tian, Yang (Department of Electronic Chemistry, Interdisciplinary Graduate School of Science and Engineering, Tokyo Institute of Technology)
Okajima, Takeyoshi (Department of Electronic Chemistry, Interdisciplinary Graduate School of Science and Engineering, Tokyo Institute of Technology)
Kitamura, Fusao (Department of Electronic Chemistry, Interdisciplinary Graduate School of Science and Engineering, Tokyo Institute of Technology)
Ohsaka, Takeo (Department of Electronic Chemistry, Interdisciplinary Graduate School of Science and Engineering, Tokyo Institute of Technology)
Publication Information
Journal of the Korean Electrochemical Society / v.5, no.4, 2002 , pp. 212-215 More about this Journal
Abstract
A superoxide dismutase (SOD)-based superoxide ion $(O_2^-)$ sensor was fabricated by immobilizing SOD on a self-assembled monolayer (SAM) of 3-mercaptopropionic acid (MPA) which was prepared on a gold electrode. The SAM of MPA was found to function as an effective promoter for the electrode reaction of SOD. The amperometric response to $(O_2^-)$ was monitored at 300mV and -100mV vs. Ag/ Agel in 5mM phosphate buffer solution containing $KO_2$. The sensor was proved to have a high sensitivity, selectivity and short response time (<5 s) and negligible interference.
Keywords
Superoxide ion; Biosensor; Superoxide dismutase; 3-mercaptopropionic acid; Self-assembled monolayer;
Citations & Related Records
연도 인용수 순위
  • Reference
1 /
[ M. Miele;M. J. Fillenz ] / Neurosci. Methods   DOI   ScienceOn
2 /
[ Y. Tian;T. Okajima;T. Ohsaka ] / manuscript in preparation
3 /
[ T. Zetterstrom;L. Vernet;U. Ungerstedt;T. B. Jonzon;B. B. Fredholm ] / Neurosci. Lett.   DOI   ScienceOn
4 /
[ K. V. Gobi;F. Mizutani ] / J. Electroanal. Chem.   DOI   ScienceOn
5 /
[ A. His;B. Liedberg ] / J. Colloid Interface Sci.   DOI   ScienceOn
6 /
[ A. Ulman ] / An Introduction to Ultrathin Organic Films-From Langmuir-Blodgett to Self-Assembly
7 /
[ T. Ohsaka;Y. Tian;M. Shioda;S. Kasahara;T. Okajima ] / Chem. Comm.
8 /
[ T. Ohsaka;Y. Tian;L. Mao;T. Okajima ] / Anal. Sci.   DOI   ScienceOn
9 /
[ B. Ge;F. Lisdat ] / Anal. Chim. Acta   DOI   ScienceOn
10 /
[ Y. Tian;L. Mao.T. Okajima;T. Ohsaka ] / Anal. Chem.   DOI   ScienceOn
11 /
[ M. L. Salin;W. W. Wilson ] / Mol. Cell. Biochem.   DOI
12 /
[ K. Kim;J. Kwak ] / J. Electroanal. Chem.   DOI   ScienceOn
13 /
[ S. Mesaros;Z. Vankova;S. Grunfeld;A. Mesarosova;T. Malinski ] / Anal. Chim. Acta   DOI   ScienceOn
14 /
[ J. M. McCord;I. Fridovich ] / J. Biol. Chem.
15 /
[ Y. Tian;M. Shioda;S. Kasahara;T. Okajima;L. Mao;T. Hisabori;T. Ohsaka ] / Biochim. Biophys. Acta   DOI   ScienceOn