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http://dx.doi.org/10.7857/JSGE.2017.22.2.041

Isolation and Identification of Bacteria Involved with Biomineralization at B Mine Sludge in Mexico  

Kim, Joon-Ha (Department of Environmental Engineering, The University of Seoul)
Yun, Seong-Yeol (Department of Environmental Engineering, The University of Seoul)
Park, Yoon Soo (Department of Environmental Engineering, The University of Seoul)
Lee, Jai-Young (Department of Environmental Engineering, The University of Seoul)
Publication Information
Journal of Soil and Groundwater Environment / v.22, no.2, 2017 , pp. 41-51 More about this Journal
Abstract
Microbial processes that bind heavy metals and form minerals are widespread, and they represent a basic aspect of biogeochemistry. Some microorganisms can crystallize minerals by secreting a specific enzyme. In particular, calcite ($CaCO_3$) precipitation is an important part of biomineralization, and has been studied extensively because of its wide application in civil engineering technology. This process provides an effective way to stabilize heavy metals within a relatively stable crystal phase. In this study, biomineralization of calcite by three urea-hydrolyzing indigenous bacterial strains was investigated by microbiological analyses. Three bacterial strains were isolated from the sludge of B mine in Mexico and each bacterial strain was identified by the cellular fatty acid composition and 16S rRNA partial sequencing analysis. The results of the identification analysis showed that these strains were closest to Sporosarcina pasteurii, Kurthia gibsonii, and Paenibacillus polymyxa. We found that the optimum conditions for growth of these indigenous bacteria were $30-40^{\circ}C$ and pH range of 7-8. Microbiological analyses showed the possibility that the bioaccumulated heavy metals ions were deposited around the cell as crystalline carbonate minerals under the optimum conditions. The findings of our study suggest that the indigenous bacterial strains play an important role in heavy metal immobilization.
Keywords
Heavy metals; Calcite; Biomineralization; Indigenous bacterial strains; Heavy metal immobilization;
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Times Cited By KSCI : 5  (Citation Analysis)
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1 Chung, J.H., Kang, P.S., Kim, C.Y., Lee, K.S., Hwang, T.Y., Kim, G.T., Park, J.S., Park, S.Y., Kim, D.S., Lim, O.T., and Sakong, J., 2005, Blood Pb, urine Cd and health assessment of residents in the vicinity of abandoned mines in Gyeongsangbukdo, Korean J. Occup. Environ. Med., 17(3), 225-237.
2 Ciurli, S., Marzadori, C., Benini, S., Deiana S., and Gessa, C., 1996, Urease from the Soil Bacterium Bacillus pasteurii : Immobilization on Ca-polygalacturonate, Soil Biol. Biochem., 28(6), 811-817.   DOI
3 Dhami, N.K., Reddy, M.S., and Mukherjee, A., 2014, Synergistic role of bacterial urease and carbonic anhydrase in carbonate mineralization, Appl. Biochem. Biotechnol., 172(5), 2552-2561.   DOI
4 Dejong, J.T., Mortensen, B.M., Martinez, B.C., and Nelson, D.C., 2010, Bio-mediated soil improvement, J. Ecol. Eng., 36(2), 197-210.   DOI
5 Jakubick, A., McKenna, G., and Robertson, A.M., 2003, Stabilisation of tailings deposits : International Experience, Proceedings of Mining and the Environment III, Sudbury, Ontario, Canada, p.1-9.
6 Kawatra, S.K. and Natarajan, K.A., 2001, Mineral biotechnology: microbial aspects of mineral beneficiation, metal extraction, and environmental control, Society for Mining, Metallugy and Exploration, inc. (SME).
7 Kim, D.H., Kim, H.C., and Park, K.H., 2010, Possibility of cementation of soft soil using Bacteria, Korean Geotech. Soc. Fall National Conference, Gyeonggi, Korea, p.379-391.
8 Kim, S.H., Cho, Y.M., Choi, S.H., Kim, H.J., and Choi, J.W., 2011, The effect of exposure factors on the concentration of heavy metals in residents near abandoned metal mines, J. Prev. Med. Public Health, 44(1), 41-47.   DOI
9 Kim, S.T., Lee, C.J., Kim, H.J., and Lee, H.C., 2012, The influence of calcite nano material on microorganism mixed mortar, J. Archit. Inst. Korea Struct. Constr., 28(5), 77-85.   DOI
10 Achal, V., Mukherjee, A., Basu, P.C., and Reddy, M.S., 2009, Strain improvement of Sporosarcina pasteurii for enhanced urease and calcite production, J. Ind. Microbiol. Biotechnol., 36(7), 981-988.   DOI
11 Gorospe, C.M., Han, S.H., Kim, S.G., Park, J.Y., Kang, C.H., Jeong, J.H., and So, J.S., 2013, Effects of different calcium salts on calcium carbonate crystal formation by Sporosarcina pasteurii KCTC 3558, Biotechnol. Bioprocess Eng., 18(5), 903-908.   DOI
12 Boquet, E., Boronat, A., and Ramos-Cormenzana, A., 1973, Production of calcite (calcium carbonate) crystals by soil bacteria is a general phenomenon, Nature, 246, 527-529.   DOI
13 Lee, J.D., Kim, T.D., Kim, S.G., and Kim, H.J., 2013, Study on the contamination characteristics of pollutants at various type of abandoned metal mines, J. Soil. Groundwater Environ., 18(3), 93-108.   DOI
14 Li, D., Nielsen, M.H., Lee, J.R.I., Frandsen, C., Banfield, J.F., and De Yoreo, J.J., 2012, Direction-specific interactions control crystal growth by oriented attachment, Science, 336(6084), 1014-1018.   DOI
15 Loewenthal, R.E. and Marais, G.V.R., 1978, Carbonate chemistry of aquatic systems: theory and application, 1, Ann Arbor Science, Ann Arbor.
16 MOE (Ministry of Environment), 2007, Residents health influence investigation of the abandoned metal mine.
17 MOE (Ministry of Environment), 2008, Health assessment of residents in the vicinity of abandoned mines.
18 MOE (Ministry of Environment), 2015, Development of hybrid immobilizing technology with indigenous bacteria and industrial waste.
19 Mitchell, J.K. and Santamarina, J.C., 2005, Biological considerations in geotechnical engineering, J. Geotech. Geoenviron. Eng., 131(10), 1222-1233.   DOI
20 Mitchell, A.C. and Ferris, F.G., 2005, The coprecipitation of Sr into calcite precipitates induced by bacterial ureolysis in artificial groundwater : temperature and kinetics dependence, Geochim. Cosmochim. Acta, 69(17), 4199-4210.   DOI
21 Mortensen, B.M., Haber, M.J., DeJong, J.T., Caslake, L.F., and Nelson, D.C., 2011, Effects of environmental factors on microbial induced calcium carbonate precipitation, J. Appl. Microbiol., 111(2), 338-349.   DOI
22 Okwadha, G.D.O. and Li, J., 2010, Optimum conditions for microbial carbonate precipitation, Chemosphere, 81(9), 1143-1148.   DOI
23 Park, K.H. and Kim, D.H., 2012, Verification of calcium carbonate by cementation of silt and sand using bacteria, J. Korean Geotech. Soc., 28(6), 53-61.   DOI
24 Park, M.J., 2014, Characterization of urease-producing bacteria and stabilization of heavy metal, J. Soil. Sci. Fertil., 47(6), 391-397.
25 Park, S.J., Park, S.H., and Ghim, S.Y., 2014, The effects of Paenibacillus polymyxa E681 on antifungal and crack remediation of cement paste, Curr. Microbiol., 69(4), 412-416.   DOI
26 Phae, C.G. and Oh, J.M., 2002, Soil contamination measurement analysis, Korea, Sinkwang-munhwasa.
27 Sarda, D., Choonia, H.S., Sarode, D.D., and Lele, S.S., 2009, Biocalcification by Bacillus pasteurii urease: a novel application, J. Ind. Microbiol. Biotechnol., 36(8), 1111-1115.   DOI
28 Seok, H.J. and Kim, C.G., 2013, Comparative assessment on indicating factor for biomineralization by Bacillus species, J. Korean Soc. Environ. Eng., 35(3), 179-191.   DOI
29 Stocks-Fischer, S., Galinat, J.K., and Bang, S.S., 1999, Microbiological precipitation of $CaCO_3$, Soil Biol. Biochem., 31(11), 1563-1571.   DOI
30 Singh, T.S. and Pant, K.K., 2006, Solidification/stabilization of arsenic containing solid wastes using Portland cement, fly ash and polymeric materials, J. Hazard. Mater., 131(1), 29-36.   DOI
31 Thompson, J.D., Higgins, D.G., and Gibson, T.J., 1994, CLUSTAL W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalies and weight matrix choice, Nucleic Acids Res., 22(22), 4673-4680.   DOI
32 United States Environmental Protection Agency (USEPA) Method 1311, 1988, Toxicity Characteristic Leaching Procedure, SW-846 : Test Methods for Evaluating Solid Waste Physical/Chemical Methods United States Environmental Protection Agency (USEPA).
33 United States Environmental Protection Agency (USEPA) Method 1312, 1994, Synthetic Precipitation Leaching Procedure, SW-846 : Test Methods for Evaluating Solid Waste Physical/ Chemical Methods United States Environmental Protection Agency (USEPA).
34 Zeynep, B.B., Mary, J.K., and Raissa, D.F., 2015, Biomineralized cement-based materials: Impact of inoculating vegetative bacterial cells on hydration and strength, Cem. Concr. Res., 67(9), 237-245.   DOI
35 Lee, S., Kim, T.H., and Lee, J.H., 2007, Soil test method, Korea, Goomibook.