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http://dx.doi.org/10.7857/JSGE.2014.19.3.015

Review of the Extraction Methods of Soil Extracts, Soil Elutriates, and Soil Suspensions for Ecotoxicity Assessments  

Nam, Sun-Hwa (Department of Environmental Health Science, Konkuk University)
An, Youn-Joo (Department of Environmental Health Science, Konkuk University)
Publication Information
Journal of Soil and Groundwater Environment / v.19, no.3, 2014 , pp. 15-24 More about this Journal
Abstract
Soil pollution has been recognized as a serious problem because it causes groundwater pollution through medium contacts. Although concentration of individual chemical could be more easily measured by physico-chemical analysis, it is not easy to consider the bioavailability of edaphic receptors living in soil or groundwater. To measure the toxicity of soil, the soil extracts (soil elutriates or soil suspensions in the other words) are often used due to the difficulties of extracting soil pore water. In this study, we reviewed 15 toxicity test methods found in literature to analyze the detail of each extraction method and to recommend the most frequently used extraction methods. The identified most commonly used extraction methods are as following: The 1 : 4 soil:water ratio, 24 hours shaking time, room temperature, dark, and separation of supernatant using a $0.45{\mu}m$ pore size filter.
Keywords
Soil elutriates; Soil extracts; Soil suspensions; Ecotoxicity assessment;
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1 Cook, S.V., Chu, A., and Goodman, R.H., 2002, Leachability and toxicity of hydrocarbons, metals and salt contamination from flare pit soil, Water Air and Soil Pollution, 133(1-4), 297-314.   DOI   ScienceOn
2 American Society for Testing and Materials (ASTM), 2009, D5660 - 96 Standard Test Method for Assessing the Microbial Detoxification of Chemically Contaminated Water and Soil Using a Toxicity Test with a Luminescent Marine Bacterium, ASTM International, United States, p. 1-9.
3 Baun, A., Justesen, K.B., and Nyholm, N., 2002, Algal test with soil suspensions and elutriates.: a comparative evaluation for PAH-contaminated soils, Chemosphere, 46(2), 251-258.   DOI   ScienceOn
4 Bowers, N., Pratt, J.R., Beeson, D., and Lewis, M., 1997, Comparative evaluation of soil toxicity using lettuce seeds and soil ciliates, Environ. Tox. Chem., 16(2), 207-213.   DOI
5 Hammel, W., Steubing, L., and Debus, R., 1998, Assessment of the Ecotoxic Potential of Soil Contaminants by Using a Soil- Algae Test, Ecotox. Environ. Saf., 40(1-2), 173-176.   DOI   ScienceOn
6 Loureiro, S., Ferreira, A.L., Soares, A.M., and Noqueira, A.J., 2005, Evaluation of the toxicity of two soils from Jales Mine (Portugal) using aquatic bioassays, Chemosphere, 61(2), 168-177.   DOI
7 Maisto, G., Manzo, S., De Nicola, F., Carotenuto, R., Rocco, A., and Alfani, A., 2011, Assessment of the effects of Cr, Cu, Ni and Pb soil comtamination by ecotoxicological tests, J. Environ. Monit., 13(11), 3049-3056.   DOI   ScienceOn
8 Marques, C.R., Pereira, R., and Gon alves, F., 2011, Toxicity evaluation of natural samples from the vicinity of rice fields using two trophic levels, Environ. Monit. Assess., 180(1-4), 521-536.   DOI
9 Anbalagan, C., Lafayett, I., Antoniou-Kourounioti, M., Haque, M., King, J., Johnsen, B., Baillie, D., Gutierrez, C., Rodriguez Martin, J.A., and De Pomerai, D., 2012, Transgenic nematodes as biosensors for metal stress in soil pore water samples, Ecotoxicology, 21(2), 439-455.   DOI
10 Anbalagan, C., Lafayett, I., Antoniou-Kourounioti, M., Gutierrez, C., Martin, J.R., Chowdhuri, D.K., and De Pomerai, D.I., 2013, Use of transgenic GFP reporter strains of the nematode Caenorhabditis elegans to investigate the patterns of stress responses induced by pesticides and by organic extracts from agricultural soils, Ecotoxicology, 22(1), 72-85.   DOI   ScienceOn
11 Arnold, R.E., Hodson, M.E., Black, S., and Davies, N.A., 2003, The influence of mineral solubility and soil solution concentration on the toxicity of copper to Eisenia fetida Savigny, Pedobiologia, 47(5-6), 622-632.
12 Antunes, S.C., Pereira, J.L., Cachada, A., Duarte, A.C., Gonalves, F., Sousa, J.P., and Pereira, R., 2010, Structural effects of the bioavailable fraction of pesticides in soil Suitability of elutriate testing, J. Hazard. Mater., 184(1-3), 215-225.   DOI   ScienceOn
13 Aruoja, V., Kurvet, I., Dubourguier, H.C., and Kahru, A., 2004, Toxicity testing of heavy-metal-polluted soils with algae Selenastrum a soil suspension assay, Environ. Toxicol., 19(4), 396-402.   DOI   ScienceOn
14 Robidoux, P.Y., Gong, P., Sarrazin, M., Bardai, G., Paquet, L., Hawari, J., Dubois, C., and Sunahara, G.I., 2004, Toxicity assessment of contaminated soils from an antitank firing range, Exotoxicol. Environ. Saf., 58(3), 300-313.   DOI   ScienceOn
15 Tiensing, T., Preston, S., Strachan, N., and Paten, G.I., 2001, Soil solution extraction techniques for microbial ecotoxicity testing: a comparative evaluation, J. Environ. Monit., 3(1), 91-96.   DOI
16 Organisation for Economic Co-operation and Development (OECD), 1998, OECD guidelines for the testing of chemicals, Daphnia sp., Acute immobilisation test.
17 Miranda, A.F.P., Rodrigues, J.M.L., Barata, C., Riva, C., Nugegoda, D., and Soares, A.M.V.M., 2011, The use of Daphnia magna immobilization tests and soil microcosms to evaluate the toxicity of dredged sediments, J. Soils Sediments, 11(2), 373-381.   DOI
18 Ore, S., Mertens, J., Brandt, K. K. and Smolders, E., 2010, Copper Toxicity to Bioluminescent Nitrosomonas europaea in Soil is explained by the free metal ion activity in pore water, Environ. Sci. Techol., 44(23), 9201-9206.   DOI
19 Power, R.S. and de Pomerai, D.I., 1999, Effect of single and paired metal inputs in soil on a stress-inducible transgenic nematode, Arch. Environ. Contam., 37(4), 503-511.   DOI   ScienceOn
20 Romkens, P.F., Bouwman, L.A., and Boon, G.T., 1999, Effect of plant growth on copper solubility and speciation in soil solution samples, Environ. Pollut., 106(3), 315-321.   DOI
21 Ronnpagel, K., Janssen, E., and Ahlf, W., 1998, Asking for the indicator function of bioassays evaluating soil contamination: are bioassay results reasonable surrogates of effects on soil microflora?, Chemosphere, 36(6), 1291-1304.   DOI
22 Thomas, M.W., Judy, B.M., Lower, W.R., Krause, G.F., and Sutton, W.W., 1990, Time-dependent toxicity assessment of herbicide contaminated soil using the green alga Selenastrum capricornutum, Plants for Toxicity Assessment, 235-254.
23 Maderova, L., Watson, M., and Paton, G.I., 2011, Bioavailability and toxicity of copper in soils: integrating chemical approaches with responses of microbial biosensors, Soil Biol. Biochem., 43(6), 1162-1168.   DOI
24 Shen, Q.R. and Shen, Z.G., 2001, Effects of pig manure and wheat straw on growth of mung bean seedlings grown in aluminium toxicity soil, Bioresour. Technol., 76(3), 235-240.   DOI