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Denitrification by a Heterotrophic Denitrifier with an Aid of Slowly Released Molasses  

Lee, Byung-Sun (Rural Research Institute, Korea Rural Community Corporation)
Lee, Kyu-Yeon (Dept. of Civil and Environmental Engineering, Seoul National University)
Shin, Do-Yun (Dept. of Civil and Environmental Engineering, Seoul National University)
Choi, Jong-Hak (Rural Research Institute, Korea Rural Community Corporation)
Kim, Young-Jin (Dept. of Civil and Environmental Engineering, Seoul National University)
Nam, Kyoung-Phile (Dept. of Civil and Environmental Engineering, Seoul National University)
Publication Information
Journal of Soil and Groundwater Environment / v.15, no.4, 2010 , pp. 30-38 More about this Journal
Abstract
This study was conducted to determine the potential applicability of slowly released molasses (SRM) to treat nitratecontaminated groundwater. SRM was made by dispersing molasses in hydroxy propyl methyl cellulose-silicamicrocrystalline cellulose matrix. Column test indicated that SRM could continuously release molasses with slowly decreasing release rates of $64.6mg-COD/L{\cdot}h$ up to 65 hrs, $12.1mg-COD/L{\cdot}h$ up to 215 hrs, and $4.4mg-COD/L{\cdot}h$up to 361 hrs. A batch test using an isolated indigenous heterotrophic denitrifier Pseudomonas sp. KY1 having nitrite reductase (nirK) and liquid molasses demonstrated that the bacterium decreased 100 mg-N/L of nitrate to less than 10 mg-N/L at the C/N ratio of 10/1 in 48 hours. In a Pseudomonas sp. KY1-attached Ottawa sand column which continuously received molasses from a SRM-containing reservoir, the bacterium successfully removed nitrate from 20 mg-N/L to 3 mg-N/L during the 361 hours of column operation. The results showed the possibility that SRM can be used as a reliable, longterm extra carbon source for indigenous heterotrophic denitrifiers.
Keywords
Slowly released molasses (SRM); Pseudomonas sp. KY1; Heterotrophic denitrification; Nitrate;
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