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Effect of Biofilm Formation on Soil Sorbed Naphthalene Degradation  

Li, Guang-Chun (Dept. of Environmental Engineering, Chonnam National University)
Chung, Seon-Yong (Dept. of Environmental Engineering, Chonnam National University)
Park, Jeong-Hun (Dept. of Environmental Engineering, Chonnam National University)
Publication Information
Journal of Soil and Groundwater Environment / v.14, no.6, 2009 , pp. 45-52 More about this Journal
Abstract
Naphthalene-degrading bacteria Pseudomonas aeruginosa CZ6 isolated from contaminated soil can adhere to crystal naphthalene and produce extracellular polymeric substance. LB, YM and MSM medium were used as culture mediums to investigate the formation of biofilm. Biofilm was developed the most in LB medium by Pseudomonas aeruginosa CZ6. In the culture, strain CZ6 growth was rarely affected by naphthalene concentration. Optimal culture condition was $30^{\circ}C$ and pH 7 at 0.10% substrate and 150 rpm shaking. The effect of culture medium on naphthalene degradation in the two soil slurry system was evaluated. The initial degradation rate of naphthalene was highest in the MSM medium of soil slurry. However, the sorbed naphthalene was rapidly degraded at the LB medium when naphthalene availability in liquid was limited. The results of this study suggest that biofilm formation and extracellular polymeric substance production increased bioavailability of soil sorbed naphthalene.
Keywords
Naphthalene; Biodegradation; Pseudomonas aeruginosa CZ6; Biofilm;
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