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Optimization of Analytical Conditions for the Quantification of Explosive Compounds in Soil using HPLC  

Cho, Jung-Hyun (Department of Civil &Environmental Engineering, Kyungwon University)
Bae, Bum-Han (Department of Civil &Environmental Engineering, Kyungwon University)
Kim, Kye-Hoon (Department of Environmental Horticulture, University of Seoul)
Publication Information
Journal of Soil and Groundwater Environment / v.14, no.1, 2009 , pp. 51-60 More about this Journal
Abstract
A series of experiments was performed to develop an optimized analytical procedure for the analysis of explosives in soil by HPLC with soil samples collected at two live-fire military shooting ranges. The minimum amount of soil to be collected, Wmin, for the analysis of explosive compounds was 125g, based on the segregation and homogeneity constants that account for soil heterogeneity and non-homogeneous distribution of target explosive compounds. The optimization of extraction and HPLC analytical conditions were also studied based on analytes CV values. The most effective soil/ extractant ratio was estimated to be 10g-pretreated soil/20 mL acetonitrile as extractant. The optimized HPLC elution conditions for the separation of US EPA designated 14 explosive compounds, were column temperature 30${\circ}C$, eluents ratio of isopropanol: acetonitrile: water = 18 : 12: 70, and flow rate of 0.8 mUmin at 230 nm. However, UV wavelength 254 nm was better for the analysis of NB, 2,4-DNT, 2NT, 4NT, and 3NT.
Keywords
Detection limit; Explosives; Extraction; HPLC; Separation;
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1 한국수자원공사, 2002, 다략대 사격장내 토양오염 정밀조사를 통한 한탄강 수질예측 및 복원공법연구
2 한국수자원공사, 2005, 군남홍수조절지 건설사업 사격장 피탄지 토양오염 정밀조사 보고서
3 Borch, T. and Gerlach, R., 2004, Use of reversed-phase high-performance liquid chromatographydiode array detection for complete separation of 2,4,6-trinitrotoluene metabolites and EPA Method 8330 explosives: Influence of temperature and an ion-pair reagent, J. of Chromatography A, 1022(1-2), 83-94   DOI   ScienceOn
4 Jenkins, T.F., Grant, C.L., Brar, G.S., Thorne, P.G, Schumacher, P.W., and Ranney, T.A., 1996b, Sample representativeness: the missing element in explosives site characterization, In: Proceedings of the American Defence Prepardness Association's 22nd Environmental Symposium and Exhibition, Orlando, Florida, p. 18-21
5 US EPA, 1992, Preparation of soil sampling protocols: Sampling techniques and strategies, Office of Research and Development, EPA/6001R-921128
6 Walsh, M.E. and Jenkins, T.F., 1992, Indetification of TNT transformation products in soil, Technical Report CRREL-SR-92-16, Cold Regions Research and Engineering Laboratory, Hanover, NH, USA
7 U.S. EPA, 1998a, Health Advisory for hexahydor-l,3,5-trinitro-1,3,5-triazine (RDX), Criteria and Standard Division, Office of Drinking Water, Washington, D.C
8 U.S. EPA, 1998b, Health Advisory for 2,4,6-trinitrotoluene (TNT), Criteria and Standard Division, Office of Drinking Water, Washington, D.C
9 Cassada, D.A., Monson, S.J., Snow, D.D., and Spalding, R.F., 1999, Sensitive determination of RDX, nitroso-RDX metabolites, and other munitions in ground water by solid-phase extraction and isotope dilution liquid chromatography-atmospheric pressure chemical ionization mass spectrometry, J. of Chromatography A, 844, 87-95   DOI   ScienceOn
10 Jenkins, T.F., Walsh, M.E., Schumacher, P.W., Miyares, P.H., Bauer, C.F., and Grant, C.L., 1989, Liquid chromatographic method for determination of extractable nitroaromatic and nitramine residues in soil, J. of the Association of Official Analytical Chemists, 72, 890-899
11 US EPA, 2006, SW 846 method 8330b: Nitroaromatics, nitramines and nitrate esters by high performance liquid chromatography (HPLC)
12 Bouvrier, E.S.P. and Oehrle, S.A., 1995, Sample preparation perspectives: SPE method development and troubleshooting, LC . GC 13, 120-125   ScienceOn
13 Hilmi, A., Long, J.H.T., and Nguyen, A.L., 1999, Determination of explosives in soil and ground water by liquid chromatography-amperometric detection, Journal of Chromatography A, 844, 97-110   DOI   PUBMED   ScienceOn
14 Crockett, A.B., Craig, H.D., Jenkins , T.F., and Sisk, W.E., 1997, Field sampling and selecting on-site analytical methods for explosives, EPA/540/R-97/501
15 US EPA, 1994, SW 846 method 8330: Nitroaromatics and nitramines by high performance liquid chromatography (HPLC)
16 Jenkins, T.F., Grant, C.L., Brar, GS., Thome, P.G, Ranney, T.A., and Schumacher, P.W., 1996a, Assessment of sampling error associated with collection and analysis of soil samples at explosive contaminated sites, Special Report 96-15, US Army Corps of Engineering, Cold Regions Research and Engineering Laboratory