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Development and Application of Acute Bioassay Method on Solid Samples using Bioluminescence Producing Bacteria  

Ko, Kyung-Seok (Groundwater & Geothermal Resources Division, Korea Institute of Geoscience & Mineral Resources)
Kong, In-Chul (School of Construction and Environmental Engineering, Yeungnam University)
Jung, Hong-Gyung (School of Construction and Environmental Engineering, Yeungnam University)
Ro, Yul (Faculty of Earth Systems and Environmental Sciences, Chonnam National University)
Publication Information
Journal of Soil and Groundwater Environment / v.12, no.6, 2007 , pp. 46-52 More about this Journal
Abstract
A toxicity method using bioluminescence producing bacteria, Escherichia coli DH5 RB1436, was developed and applied on solid environmental samples. In the assay, 1 g soil sample was mixed with 4 ml RB1436 strain. Sets amended with p-buffer were employed for control in soil test, showing approximately 108% of sets amended with combusted soils. Measurable differences were observed between relatively more polluted groups (HP) and less polluted groups (LP) of soil samples, showing average toxicity 43 and 26%, respectively, in direct soil toxicity test. $EC_{50}$'s for all soil groups appeared in the range of $1.8{\sim}4.6\;g$, but those of sediments from dam reservoir and refuses were below 0.22 g. This developed bioassay should prove useful as a screening test for toxicity in various types of environmental solid samples.
Keywords
Bioluminescence; Solid environmental samples; Toxicity; Bioassay;
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