Browse > Article

Use of Activated Soil to Bioaugment Degradation of Atrazine in Soils  

Kim, Sang-Jun (Dept. of Natural Sciences, Republic of Korea Naval Academy)
Publication Information
Journal of Soil and Groundwater Environment / v.11, no.6, 2006 , pp. 43-52 More about this Journal
Abstract
Effectiveness of activated soil containing directly enriched atrazine-degrading soil microorganisms as an inoculant to bioaugment degradation of atrazine in soils was investigated. A Wooster silt loam (Typic Fragiudalf) was spiked with atrazine at a rate of 4 mg/kg soil three successive times to create activated soil. Atrazine degradation was significantly enhanced (p < 0.05) after the first treatment. After the second treatment, there was an increase in the number, based on MPN, of microorganisms utilizing atrazine as a C- and N-source by 3 logs and 1 log of magnitude, respectively. Inoculation of typical agricultural soils collected from Ohio with activated soil at a rate as low as 0.5% reduced the extractable atrazine remaining in soils to the level below 2% of that initially recovered (initially added at a rate of 4 mg/kg soil) after 4 days. Inoculation at a higher rate was required to achieve the same result in soils with non-typical properties (pH of 4.5 or organic matter of 43% w/w). Activated soil was stable, in terms of atrazine degradation activity, at least up to 6 months when it was kept at low temperature (< $10^{\circ}C$) and moistened (water content above 15%). The results of this study indicate that microorganisms capable of degrading atrazine are relatively easily enriched in soil to create activated soil. Use of activated soil can be a practical option for bioremediation of contaminated soils.
Keywords
Atrazine; Bioaugmentation; Bioremediation; Activated soil; Pesticide;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Alexander, M., 1982, Most probable number method for microbial populations, In: Page, A.L., Miller, R.H., and Keeney, D.R. (eds.), Methods of Soil Analysis, 2nd ed., Part 2, American Society of Agronomy, Madison, WI, p. 815-820
2 Bouquard, C., Ouazzani, J., Prome, J.-C., Michel-Briand, Y., and Plesiat, P., 1997, Dechlorination of atrazine by a Rhizobium sp. Isolate, Appl. Environ. Microbiol, 63, 862-866
3 Donneley, P.K., Entry, J.A., and Crawford, D.L., 1993, Degradation of atrazine and 2,4-dichlorophenoxyacetic acid by mycorrhizal fungi at three nitrogen concentrations in vitro, Appl. Environ. Microbiol., 59, 2642-2647
4 Gannon, E., 1992, Site remediation: environmental clean-up of fertilizer and agri-chemical dealer sites, 28 Iowa case studies, Iowa Natural Heritage Foundation, Des Moines, Iowa
5 Goodrich, J.A., Lykins, Jr., B.W., and Clark, R.M., 1991, Drinking water from agriculturally contaminated groundwater, J. Environ. Qual., 20, 707-717   DOI
6 Koelliker, J.K., Steichen, J.M., and Grosh, D.L., 1986, Pollution of ground water in Kansas, In: Erickson, L.E. (ed.), Proceedings of the Conference on Hazardous Waste Research, Kansas State University, Manhattan, KS
7 Thurman, E.M., Goolsby, D.A., Meyer, M.T., Mills, M.S., Pomes, M.L., and Kolpin D.W., 1992, A reconnaissance study of herbicides and their metabolites in surface water of the Midwestern United States using immunoassay and gas chromatography/mass spectrometry, Environ. Sci. Technol., 26, 2440-2447   DOI
8 Struthers, J.K., Jayachandran, K., and Moorman, T.B., 1998, Biodegradation of atrazine by Agrobacterium radiobacter J14a and use of this strain in bioaugmentation of contaminated soil, Appl. Environ. Microbiol., 64, 3368-3375
9 Chung, K.M., Ro, K.S., and Roy, D., 1996, Fate and enhancement of atrazine biotransformation in anaerobic wetland sediment, Wat. Res., 30, 341-346   DOI   ScienceOn
10 Buoyoucos, G.J., 1962, Hydrometer method improved for making particle-size analysis of soils, Agron. J., 53, 464-465
11 Runes, H.B., Jenkins, J.J., and Bottomley, P.J., 2001, Atrazine degradation by bioaugmented sediment from constructed wetlands, Appl. Microbiol. Biotechnol., 57, 427-432   DOI
12 Schoen, S.R. and Winterlin, W.L., 1987, The effects of various factors and amendments on the degradation of pesticide mixtures, J. Environ. Sci. Health, Part B, 22, 347-377   DOI   ScienceOn
13 Newcombe, D.A. and Crowley, D.E., 1999, Bioremediation of atrazine-contaminated soil by repeated application of atrazine degrading bacteria, Appl. Microbiol. Biotechnol., 51, 877-882   DOI
14 Yanze Kontchou, C. and Gschwind, N., 1996, Mineralization of the herbicide atrazine in soil inoculated with a Pseudomonas strain, J. Agric. Food Chem., 43, 2291-2294   DOI   ScienceOn
15 Behki, R.M., Topp, E., Dick, W., and Germon, P., 1993, Metabolism of the herbicide atrazine by Rhodococcus strains, Appl. Environ. Microbiol, 59, 1955-1959
16 Torsvik, V., Goksoyr, J., and Daae, F.L., 1990, High diversity in DNA of soil bacteria, Appl. Environ. Microbiol., 56, 782-787
17 Rousseaux, S., Hartmann, A., Lagacherie, B., Piutti, S., Andreux, F., and Soulas, G., 2003, Inoculation of an atrazinedegrading strain, Chelatobacter heintzii Citl, in four different soils: effects of different inoculum densities. Chemosphere, 51, 569-576   DOI   ScienceOn
18 Mandelbaum, R.T., Wackett, L.P., and Allan, D.L., 1993, Mineralization of the s-triazine ring of atrazine by stable bacterial mixed cultures, Appl. Environ. Microbiol., 59, 1695-1701
19 Topp, E., 2001, A comparison of three atrazine-degrading bacteria for soil bioremediation, Biol. Fert. Soils., 33, 529-534   DOI
20 Grigg, B.C., Assaf, N.A., and Turco, R.F., 1997, Removal of atrazine contamination in soil and liquid systems using bioaugmentation, Pestic. Sci., 50, 211-220   DOI   ScienceOn
21 Gupta, G. and Baummer, III, J., 1996, Biodegradation of atrazine in soil using poultry litter, J. Hazard. Materials, 45, 185-192   DOI   ScienceOn
22 Assaf, N.A. and Turco, R.F., 1994, Accelerated biodegradation of atrazine by a microbial consortium is possible in culture and soil, Biodegradation, 5, 29-35
23 McKeague, J.A.(ed.), 1978, Manual on soil sampling and methods of analysis, Can. Soc. Soil Sci.
24 Behki, R.M. and Khan, S.U., 1986, Degradation of Atrazine by Pseudomonas: N-dealkylation and dehalogenation of atrazine and its metabolites, J. Agric. Food. Chem, 34, 746-749   DOI
25 Combs, S.M. and Nathan, M.V., 1998, Recommended Chemical Soil Test Procedures for the North Central Region, NCR Publication No. 221, Missouri Agricultural Experiment Station, Columbia, MO, p. 53-58
26 Mougin, C., Laugero, C., Asther, M., and Chaplain, V. 1997, Biotransformation of s-triazine herbicides and related degradation products in liquid cultures by the white rot fungus Phanerochaete chrysosporium, Pestic. Sci., 49, 169-177   DOI   ScienceOn
27 Gan, J., Becker, R.L., Koskinen, W.C., and Buhler, D.D., 1996, Degradation of atrazine in two soils as a function of concentration, J. Environ. Qual., 25, 1064-1072   DOI   ScienceOn
28 Barriuso, E. and Houot, S., 1996, Rapid mineralization of the striazine ring of atrazine in soils in relation to soil management, Soil. Biol. Biochem, 28, 1341-1348   DOI   ScienceOn